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EC number: 213-156-1 | CAS number: 927-62-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 6 October 1988 to 8 December 1988
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.1 (Acute Toxicity for Fish)
- Version / remarks:
- Cited as Directive 84/449/EEC, C.1 ("Acute toxicity for fish")
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Deviations:
- yes
- Remarks:
- test substance not measured
- GLP compliance:
- yes
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: the test substance was added to an aliquot of test water and dissolved using an ultra-turrax and ultrasonic bath. The stock solution was then dissolved in the test vessel. This resulted in pH-values in the range 9.8 to 10.6. The pH was adjusted with 0.64 to 2.6 mL of 1 n HCL, the exact volume depending on the respective dose level.
- Evidence of undissolved material: yes. Precipitate at 250 and 500 mg/L. - Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- TEST ORGANISM
- Common name: zebra fish
- Strain: brachidanio rerio (Hamilton-Buchanan)
- Source: West Aquarium, Bad Lauterberg, Germany)
- Age at study initiation: approx. 15 weeks
- Length at study initiation (length definition, mean, range and SD): 2.8 cm (range 2.4 - 3.1 cm), SD 0.17 cm
ACCLIMATION
- Acclimation period: min 14 days
- Acclimation conditions: same as during testing. Temperature 22 ± 1 °C; light/dark 12/12 hours; oxygen saturation min 80 %
- Type and amount of food: Tetra Min, standard diet ad libitum
- Feeding frequency: twice daily - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Post exposure observation period:
- n.a.
- Hardness:
- reconstituted water according to ISO/DIS 7346/1 was used
- Test temperature:
- 22 ± 1 °C
- pH:
- 7.5 to 8.1 (after neutralisation)
- Dissolved oxygen:
- 7.1 to 9.0 mg/L
- Nominal and measured concentrations:
- 0, 100, 125, 180, 250, and 500 mg/L
- Details on test conditions:
- TEST SYSTEM
- Type (open / closed): no data
- Material, size, headspace, fill volume: glass, fill volume 10 litres
- Aeration: static, no aeration during the test
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 4
- Biomass loading rate: 1 fish/L
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: reconstituted water according to ISO/DIS 7346/1
- Culture medium different from test medium: no
- Intervals of water quality measurement: at 0, 2-4, 24, 48, 72, and 96 hours after initiation.
OTHER TEST CONDITIONS
- Adjustment of pH: yes; the pH was adjusted with hydrochloric acid. The results refer therefore to the nominal concentrations of the neutralised test substance.
- Photoperiod: 12 hours/day
- Light intensity: 700 lux
EFFECT PARAMETERS MEASURED
- behavior and mortality was determined at 2-4, 24, 48, 72, and 96 hours after initiation.
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 1.41 - Reference substance (positive control):
- no
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 212 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Details on results:
- see below
- Results with reference substance (positive control):
- n. a.
- Reported statistics and error estimates:
- The LC50 was 212 mg/L (95 % CI: 189 - 239 mg/L).
- Sublethal observations / clinical signs:
Mortality
Dose level (mg/L)
Number of dead animals
48 h
96 h
0
0/40
0/40
100
0/10
0/10
125
0/10
0/10
180
0/10
1/10
250
5/10
9/10
500
10/10
10/10
The LC50 was 212 mg/L (95 % CI: 189 - 239 mg/L)
CLINICAL SYMPTOMS
No observation of high-dosed groups was possible due to rapid death of the animals.
The 125-mg/L group showed normal behaviour and appearance.
The animals of the 180- and 250-mg/L groups, but also at 100 mg/L, showed hypoactivity, increased respiration rate, extended gill covers, swimming near the surface, dark discolouration.- Validity criteria fulfilled:
- yes
- Conclusions:
- The 96-hr LC50 was 212 mg/L in a GLP guideline study using zebra fish (neutralised test substance)
- Executive summary:
The acute toxicity to fish was examined in a GLP and guideline-compliant study (OECD 203, EU C3) using Zebra fish (10/dose). The dose levels were 0, 100, 125, 180, 250, and 500 mg/L. Precipitation and high pH-values were observed after the addition of stock solutions to the vessels. Consequently, the pH was adjusted with hydrochloric acid, but no analytical monitoring of the test substance was performed. The results refer therefore to the nominal concentrations of the neutralised test substance.
Symptoms were seen at 100, 180 and 250 mg/L. 10 % mortality was seen at 180 mg/L, and 90 % at 250 mg/L, i.e. there was a steep dose-mortality curve. The LC50 was determined to be 212 mg/L (Markert and Jung, 1988).
The study is considered to be valid for the assessment of the neutralised test substance.
Reference
Description of key information
The LC50 was determined to be 212 mg/L.
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 212 mg/L
Additional information
The acute toxicity to fish was examined in a GLP and guideline-compliant study (OECD 203, EU C3) using Zebra fish (10/dose). The dose levels were 0, 100, 125, 180, 250, and 500 mg/L. Precipitation and high pH-values were observed after the addition of stock solutions to the vessels. Consequently, the pH was adjusted with hydrochloric acid, but no analytical monitoring of the test substance was performed. The results refer therefore to the nominal concentrations of the neutralised test substance.
Symptoms were seen at 100, 180 and 250 mg/L. 10 % mortality was seen at 180 mg/L, and 90 % at 250 mg/L, i.e. there was a steep dose-mortality curve. The LC50 was determined to be 212 mg/L (Markert and Jung, 1988).
The study is considered to be valid for the assessment of the neutralised test substance.
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