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EC number: 217-682-2 | CAS number: 1929-82-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to soil macroorganisms except arthropods
Administrative data
Link to relevant study record(s)
Description of key information
15-day LC50 = 209 mg/kg dw (earthworm); EPA OPPTS 850.6200, Coulson et al. (1985)
56-day NOEC = 5.75 mg/kg soil dw (earthworm); OECD 222, ISO 11268-2, Ganßmann (2013)
Key value for chemical safety assessment
- Short-term EC50 or LC50 for soil macroorganisms:
- 209 mg/kg soil dw
- Long-term EC10, LC10 or NOEC for soil macroorganisms:
- 5.75 mg/kg soil dw
Additional information
Short term
The toxicity of the test material to earthworms was investigated in a study which was conducted under GLP conditions and in accordance with the standardised guideline EPA OPPTS 850.6200. The study was assigned a reliability score of 1 in line with the criteria of Klimisch et al. (1997).
During the study, Eisenia fetida were exposed to a series of concentrations of test material evenly incorporated into an artificial soil substrate. Worms were assessed for mortality and behavioural effects after 14 days in the initial test and after 7 and 15 days in the definitive test. The soil was tipped onto a sheet of 'Benchkote' and worms were recorded as dead if there was no response to a mechanical stimulus of their front end. Each batch of ten earthworms was weighed at the end of the test to determine any treatment related effect.
The highest concentration with no mortality was 100 mg/kg. The lowest concentration giving 100 % mortality was 320 mg/kg.
Control mortality was zero. No significant behavioural or bodyweight changes were observed at or below 180 mg/kg level.
Under the conditions of the study the 15-day LC50 value for the test material, to earthworms, was determined to be 209 mg/kg.
Long term
The long term toxicity of the test material to earthworms was investigated in a study which was conducted under GLP conditions and in accordance with the standardised guidelines OECD 222 and ISO 11268 -2.
During the study adult Eisenia fetida andrei were exposed to artificial soil which had been spiked with test material solution or vehicle control. There were 8 replicates per control and 4 replicates per test material treatment group; each replicate contained 10 earthworms. All worms were washed with tap water, dried with dry paper towels, weighed individually and randomly assigned to batches of 10 worms. The different batches were sorted into four classes on the basis of the total weight and one batch of each weight class was assigned to each treatment group (two batches for the control) to ensure weights were homogeneous. The earthworms were placed on the surface of the artificial soil after application.
After 4 weeks, the artificial soil was transferred to a tray and adult worms were counted, removed and weighed per replicate after they had been washed under tap water and dried on a dry paper towel. Missing earthworms and earthworms that failed to respond to gentle stimulation were considered to be dead. The remaining soil (without the adult worms) was then returned to the respective test containers.The offspring were then exposed for an additional 4 weeks after which time the juveniles were removed by placing the test units in a water bath at 50 to 60°C and counting all emerging worms. In addition the soil of each container was transferred to a tray and checked visually for any remaining young worms.
Under the conditions of the study no mortality was observed in any test material group or the control. The body weight of the earthworms exposed to test material were not statistically significantly different compared to the control up to and including the highest test concentration of 5.75 mg test item/kg soil. The reproduction rates of the worms were not statistically significantly different at any concentration tested when compared to the control.
The LC50 was therefore estimated to be greater than 5.75 mg test material/kg soil. The No Observed Effect Concentration (NOEC) for mortality of the earthworm Eisenia fetida was determined to be 5.75 mg test material/kg soil. The No Observed Effect Concentration (NOEC) for weight changes was determined to be the concentration of 5.75 mg test material/kg soil. The No Observed Effect Concentration (NOEC) for reproduction was determined to be 5.75 mg test material/kg soil.
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