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EC number: 220-621-2 | CAS number: 2835-99-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
Description of key information
In Fish Embryo Acute Toxicity test, the 96 hour LC50 of 4-Amino-m-cresol to Danio rerio (zebrafish) was 0.42 mg/L based on time-weighted mean measured concentration.
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 0.42 mg/L
Additional information
The Fish Embryo Acute Toxicity test of 4-Amino-m-cresol to Danio rerio (zebrafish) was determined under semi-static conditions, for 96h, following the OECD guideline 236 and the GLP.
The dilution water used for conducting the tests was treated mains water that had been passed through activated carbon filters. The test vessels were 24-well polystyrene microtiter plates. A single plate was used for the control, each test concentration and the positive control. Twenty wells on each plate were filled with 2 mL of the appropriate control or test media. The remaining four wells in each plate were filled with treated mains water to act as internal controls.
The tested substance concentration was analysed by LC-MS/MS on new and old media. The nominal tested concentrations were 0 (control), 0.19, 0.41, 0.91, 2, 7.5 and 10 mg/L. The tested concentrations decrease quickly before the media renewal. Analysis of the tested solutions at 0 and 48 hours (new media) showed measured concentrations to range from 68% to 117% of nominal. Analysis of the tested solutions at 24 and 72 hours (old media) showed measured concentrations to range from 2% to 66% of nominal. The concentrations were expressed as time-weighted mean measured concentrations : 0.12; 0.23; 0.39; 0.71; 1.1 and 2.5 mg/L and this range of concentrations induces 75%, 95%, 50%; 0%, 0%, 0% of eggs survival respectively, and 90% in the control. The mortality is based on the observation of at least one of the following criteria : coagulation of the egg, absence of somite formation, non-detachment of the tail and lack of heart beat.
The 96 hour LC50 of 4-Amino-m-cresol to Danio rerio (zebrafish) was 0.42 mg/L based on time-weighted mean measured concentration..
All the validity criteria were fulfilled except the temperature which was not maintained at 26 ± 1°C during the test . However this deviation was considered as not having any impact on the results since the control embryo overall survival and hatching met the validity criteria.
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