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EC number: 210-894-6 | CAS number: 625-45-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
Data source
Reference
- Reference Type:
- publication
- Title:
- Mutagenicity of ethylene glycol ethers and of their metabolites in Salmonella typhimurium his-
- Author:
- Hoflack JC, Lambolez L, Elias Z, Vasseur P
- Year:
- 1 995
- Bibliographic source:
- Mutat Res., 341: 281-287.
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Deviations:
- not applicable
- GLP compliance:
- not specified
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- Methoxyacetic acid
- EC Number:
- 210-894-6
- EC Name:
- Methoxyacetic acid
- Cas Number:
- 625-45-6
- Molecular formula:
- C3H6O3
- IUPAC Name:
- 2-methoxyacetic acid
- Reference substance name:
- 2-methoxyacetic acid
- IUPAC Name:
- 2-methoxyacetic acid
- Details on test material:
- - Name of test material: Methoxyacetic acid (obtained from Merck, Darmstadt, Germany)
- Molecular weight: 90.08
- Analytical purity: > 99 %
- Physical state: liquid at ambient temperature
- Impurities: less than 0.005% peroxides and less than 0.001% aldehydes (data from manufacturer
- Purity: test compound was checked for purity by high definition GC/MS and NMR. Merck products contained less than
0.005% peroxides and less than 0.001% aldehydes (data from manufacturer).
- Expiration date: compounds were used for testing within 6 months after receipt or 1 month after organic synthesis.
- Storage condition: test material was stored in the dark at + 4°C in air-tight vials under nitrogen and in sterile conditions
Constituent 1
Constituent 2
Method
- Target gene:
- In the Salmonella typhimurium strains (TA 97a, TA 98, TA 100, TA102) the amino acid histidine locus is the target gene, in which induced back mutations will transform the histidine auxotrophy (his-) to histidine prototrophy (his+). The Salmonella typhimurium histidine (his) reversion system measures his- => his+ reversions. The Salmonella typhimurium strains are constructed to differentiate between several base pair (TA 100, TA 102) and frameshift (TA 97a, TA 98) mutations.
Species / strain
- Species / strain / cell type:
- S. typhimurium, other: TA 97a, TA 98, TA100, TA102
- Additional strain / cell type characteristics:
- other: All strains have a defecient excision repair system (uvrB), which results in greatly enhanced sensitivity to some mutagens and considerably reduced hydrophilic polysaccharide layer (rfa), which leads to an increase in permeability to lipophilic substances
- Metabolic activation:
- with and without
- Metabolic activation system:
- S9-mix (liver homogenate)
- Test concentrations with justification for top dose:
- Methoxyacetic acid was tested up to cytotoxic concentrations.
Up to 26 µmol/plate Methoxyacetic acid (2 mg/plate) were used. - Vehicle / solvent:
- - Vehicle(s)/solvent(s) used: ultrapure sterile water, DMSO
Controlsopen allclose all
- Untreated negative controls:
- yes
- Remarks:
- (= solvent controls)
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- not specified
- Positive controls:
- yes
- Remarks:
- , used in assay with S9-mix (+ S9-mix)
- Positive control substance:
- benzo(a)pyrene
- Remarks:
- Positive control substance was dissolved in DMSO.
Migrated to IUCLID6: , 1 µg/plate, strain: TA 97a, TA 98, TA 100
- Untreated negative controls:
- yes
- Remarks:
- (= solvent controls)
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- not specified
- Positive controls:
- yes
- Remarks:
- , used in assay with S9-mix (+ S9-mix)
- Positive control substance:
- other: DHAC, 50 µg/plate, strain: TA 102
- Remarks:
- 1,8-dihydroxyanthrachinon (DHAC) was dissolved in DMSO.
- Untreated negative controls:
- yes
- Remarks:
- (= solvent controls)
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- not specified
- Positive controls:
- yes
- Remarks:
- , used in assay with without S9-mix (- S9-mix)
- Positive control substance:
- 9-aminoacridine
- Remarks:
- Positive control substance was dissolved in DMSO.
Migrated to IUCLID6: , 40 µg/plate, strain: TA 97a
- Untreated negative controls:
- yes
- Remarks:
- (= solvent controls)
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- not specified
- Positive controls:
- yes
- Remarks:
- , used in assay with without S9-mix (- S9-mix)
- Positive control substance:
- 2-nitrofluorene
- Remarks:
- Positive control substance was dissolved in DMSO.
Migrated to IUCLID6: , 0.5 µg/plate, strain: TA 98
- Untreated negative controls:
- yes
- Remarks:
- (= solvent controls)
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- not specified
- Positive controls:
- yes
- Remarks:
- , used in assay with without S9-mix (- S9-mix)
- Positive control substance:
- sodium azide
- Remarks:
- Positive control substance was dissolved in ultrapure water.
Migrated to IUCLID6: , 2.5 µg/plate, strain: TA 100
- Untreated negative controls:
- yes
- Remarks:
- (= solvent controls)
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- not specified
- Positive controls:
- yes
- Remarks:
- , used in assay with without S9-mix (- S9-mix)
- Positive control substance:
- cumene hydroperoxide
- Remarks:
- Positive control substance was dissolved in DMSO.
Migrated to IUCLID6: , 75 µg/plate, strain: TA 102
- Details on test system and experimental conditions:
- METHOD OF APPLICATION: Overlay (soft) agar (plate incorporation method). The glucose concentration of base- and top-agars was 2%. Both agar types were used within 36 h after preparation to avoid dehydratation/increase of glucose concentration.
DURATION
- Preincubation period: No preincubation was performed. The dilutions of the compounds in ultrapure water were added to the top agar to an extent of 0.1 mL/plate, and the solutions of the chemicals diluted in DMSO to an extent of 0.2 mL/plate. After mixing of all components, soft agar was distributed directly on the plates.
- Exposure duration / Expression time / Selection time: Plates with microorganisms / test item situated in the topagar and controls were incubated at 37 °C and colonies were counted after 48 h incubation.
SELECTION AGENT (mutation assays): The selection is done by using a minimal agar that contains no histidine for the Salmonella typhimurium strains.
NUMBER OF REPLICATIONS: Three or four separate experiments were performed for each compound and each concentration was tested in triplicate plates. - Evaluation criteria:
- The test substance is considered positive in this assay if the following criteria are met:
- Doubling of the revertant colonies compared to solvent control
- Presence of a dose-effect relationship - Statistics:
- No data given.
Results and discussion
Test resultsopen allclose all
- Species / strain:
- S. typhimurium TA 97a
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- Methoxyacetic acid conc. > 2 mg/plate
- Vehicle controls validity:
- not specified
- Untreated negative controls validity:
- not specified
- Positive controls validity:
- not specified
- Species / strain:
- S. typhimurium TA 98
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- Methoxyacetic acid conc. > 2 mg/plate
- Vehicle controls validity:
- not specified
- Untreated negative controls validity:
- not specified
- Positive controls validity:
- not specified
- Species / strain:
- S. typhimurium TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- Methoxyacetic acid > 2 mg/plate
- Vehicle controls validity:
- not specified
- Untreated negative controls validity:
- not specified
- Positive controls validity:
- not specified
- Species / strain:
- S. typhimurium TA 102
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- Methoxyacetic acid concentration > 2 mg/plate
- Vehicle controls validity:
- not specified
- Untreated negative controls validity:
- not specified
- Positive controls validity:
- not specified
- Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Applicant's summary and conclusion
- Executive summary:
In a reverse gene mutation assay in bacteria (Hoflack et al., 1995), strains TA 97a, TA 98, TA 100 and TA 102 of S. typhimurium were exposed to Methoxyacetic acid (> 99 % a.i.) at concentrations up to cytotoxicity of 2 mg/plate (vehicle: sterile water) in the presence and absence of mammalian metabolic activation (S9-mix) in the standard plate test (plate co-incubation). Methoxyacetic acid gave negative responses with every strain used, either with or without metabolic activation.
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