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EC number: 255-490-0 | CAS number: 41672-81-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2013-06-09 to 2013-07-02
- Reliability:
- 1 (reliable without restriction)
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations:
- Sampling method: Water samples (duplicate per condition) were taken from the control and all surviving test groups at 0 (frexh media), 24 (olf media), 72 (fresh media) and 96 (old media) hours for quantitative analysis.
- Sample storage conditions before analysis: -20°C - Vehicle:
- no
- Details on test solutions:
- Amounts of test item (210, 378, 672, 1176 and 2100 mg) were each serarately added to the surface of 21 liters of dechlorinated tap water to give the 10, 18, 32, 56 and 100 mg/L loading rates respectively. After the addition of the test item, the dechlorinated tap water was stirred by magnetic stirrer using a stirring rate such that a vortex formed to give a dimple at the water surface. The stirring was stopped after 23 hours and the mixtures allowed to stand for 1 hour. After standing, the WAFs were removed by filtration through a 0.2 µm Sartorius Sartropore filter (initial 1 liter discarded) to give the 10, 18, 32, 56 and 100 mg/L loading rates respectively.
- Test organisms (species):
- Oncorhynchus mykiss (previous name: Salmo gairdneri)
- Details on test organisms:
- TEST ORGANISM
- Common name: rainbow trout
- Strain: Oncorhynchus mykiss
- Source: Brow Well Fisheries Limited, Hebden, near Skipton, Yorkshire, UK
- Age at study initiation (mean and range, SD):
- Length at study completion (length definition, mean, range and SD): 4.7 cm (sd=0.1)
- Weight at study completion (mean and range, SD): 1.37 cm (sd=0.11)
- Feeding during test
- Food type: commercial fish food
- Amount: on demand
- Frequency: regularly, the diet was discontinued 24 hours prior testing
ACCLIMATION
- Acclimation period: 12days
- Acclimation conditions (same as test or not): same
- Health during acclimation (any mortality observed): 0 mortality during 7 days prior to the start
QUARANTINE (wild caught)
Fish were maintained in a glass fiber tank with a "single pass" water renewal system. - Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Post exposure observation period:
- determined 3 and 6 hours after the start of exposure and then daily throughout the test until termination after 96 hours.
- Hardness:
- approximately 140 mg/L as CaCO3
- Test temperature:
- 14°C to 16°C
- pH:
- between 7.5 and 8.3
- Dissolved oxygen:
- between 9.1 and 10.2 mg O2/L
- Salinity:
- not availbale
- Nominal and measured concentrations:
- nominal concentrations : 10, 18, 32, 56 and 100 mg/L
Measured concentrations : < LOQ - Details on test conditions:
- no additional detail
- Reference substance (positive control):
- no
- Duration:
- 3 h
- Dose descriptor:
- LL50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 6 h
- Dose descriptor:
- LL50
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: Mortalities (7) were only observed at 56 mgL
- Duration:
- 24 h
- Dose descriptor:
- LL50
- Effect conc.:
- >= 18 - <= 32 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 48 h
- Dose descriptor:
- LL50
- Effect conc.:
- >= 18 - <= 32 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 72 h
- Dose descriptor:
- LL50
- Effect conc.:
- >= 18 - <= 32 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LL50
- Effect conc.:
- 24 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: with 95% confidence limits of 18 -32 mg/L loading rate WAF.
- Details on results:
- no additional detail
- Results with reference substance (positive control):
- not applicable
- Validity criteria fulfilled:
- yes
- Conclusions:
- The acute toxicity of the test item to the freshwater fish rainbow trout (Oncorhynchus mykiss) has been investigated and gave a 96-hour LL50 value of 24 mg/L loading rate WAF with 95% confidence limits of 18-32 mg/L loading rate WAF. The Lowest Observed Effect Loading rate was 32 mg/L loading rate WAF. The no Observed Effect Loading rate was 18 mg/L loading rate.
- Executive summary:
Introduction
A study was performed to assess the acute toxicity of the test item to rainbow trout (Oncorhynchus mykiss). The method followed was designed to be compatible with the OECD Guidelines for testing of Chemicals (1992) N° 203, "Fish, Acute Toxicity test" referenced as Method C.1 of Commission Regulation (EC) N° 440/2008.
Method
Due to the low aquous solubility and the complex mixture of the test item for the purposes of the test, the test item was prepared as a Water Accommodation Fraction (WAF).
Following a preliminary range-finding test, fish were exposed, in groups of seven, to Water Accomodation Fractions (WAFs) of the test item over a range of nominal loading rates of 10, 18, 32, 56 and 100 mg/L for a period of 96 hours at a temperature of 14°C to 16 °C under semi-static test conditions. The number of mortalities and any sub-lethal effects of exposure in each test and control vessel were determined 3 and 6 hours after the start of exposure and then daily throughout the test until termination after 96 hours.
Results
The 96 -hours LL50 based on nominal loading rates was 24 mg/L loading rate WAF with 95% confidence limits of 18 -32 mg/L loading rate WAF. The No Observed Effect Loading rate was 18 mg/L loading rate WAF.
Chemical analysis of the test preparations at 0, 24, 72 and 96 hours showed measured test concentrations of less than the limit of quantification (LOQ) of the analytical mathod employed were obtained which was determined to be 0.078mg/L. This does not infer that no test item was in solution, just that any dissolved test item was at a concentration of less than the LOQ.
Given that toxicity cannot de attributed to a single component or a mixture of components but to the test item as a whole, the resultst were based on nominal loading rates only.
Reference
Description of key information
LC50 = 24mg/L
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Dose descriptor:
- LC50
- Effect concentration:
- 24 mg/L
Additional information
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