Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 221-717-7 | CAS number: 3209-22-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Bioaccumulation: aquatic / sediment
Administrative data
Link to relevant study record(s)
- Endpoint:
- bioaccumulation in aquatic species: fish
- Type of information:
- experimental study
- Adequacy of study:
- other information
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
- Principles of method if other than guideline:
- The bioconcentration factor of a series of nitrobenzenes derivatives was determined for the guppy.
- GLP compliance:
- not specified
- Radiolabelling:
- no
- Details on sampling:
- - Sampling intervals/frequency for test organisms: After 1 and 2 days of exposure two guppies were taken for analysis; at the end of day three all remaining fish were taken.
- Test organisms (species):
- Poecilia reticulata
- Details on test organisms:
- TEST ORGANISM
- Common name: guppy
- female guppies were used
- Age at study initiation: 5 to 8 month old
- Weight at study initiation: wet weight varied from 60 to 450 mg at the end of the experiment; mean fat content was 8 +/-2 %
- Food type: commercial fishfood
- Frequency: daily
ACCLIMATION
- Acclimation period: at least 12 days - Route of exposure:
- aqueous
- Test type:
- semi-static
- Water / sediment media type:
- natural water: freshwater
- Total exposure / uptake duration:
- 3 d
- Hardness:
- 25 mg/l CaCO3
- Details on test conditions:
- TEST MEDIUM / WATER PARAMETERS
- water for all experiments: standard water (SW) prepared according to Alabaster and Abram (1964), with corresponds to very soft tap water (hardness: 25 mg/l as CaCO3)
TEST SYSTEM
- Test vessel: glas jars of 1.5 l containing 1 l of solution
- Renewal rate of test solution: daily
- No. of organisms per vessel: 9 female guppies
TEST MEDIUM / WATER PARAMETERS
- water for all experiments: standard water (SW) prepared according to Alabaster and Abram (1964), with corresponds to very soft tap water (hardness: 25 mg/l as CaCO3) - Reference substance (positive control):
- not specified
- Key result
- Type:
- BCF
- Value:
- 1 027 dimensionless
- Basis:
- whole body w.w.
- Remarks on result:
- other: Conc.in environment / dose:840 µg/l
- Type:
- BCF
- Value:
- 82 dimensionless
- Basis:
- total lipid content
- Remarks on result:
- other: average lipid content: 8 +/- 2 %
- Remarks:
- Conc.in environment / dose:840 mg/l
- Conclusions:
- The bioaccumulation factor (BCF) was determined in an experimental study. Guppies (poecilia reticulata) were exposed to 1,2-dichloro-3-nitrobenzene for 3 days under semi-static conditions in freshwater. A BCF of 1027 +/-95 (N=7); whole body w.w. was determined.
- Executive summary:
Deneer, 1987
The bioaccumulation factor (BCF) was determined in an experimental study. Guppies (poecilia reticulata) were exposed to 1,2-dichloro-3-nitrobenzene for 3 days under semi-static conditions in freshwater.
BCF: 1027 +/-95 (N=7); whole body w.w.
log BCF: 3.01 +/- 0.04; whole body w.w.
BCF: 82 +/- 20; total lipid content
In same preliminary experiments it was established that the uptake of the chemicals proceeded very fast, thus enabling the use of this short-term assay.
Reference
results:
BCF: 1027 +/-95 (N=7); whole body w.w.
log BCF: 3.01 +/- 0.04; whole body w.w.
BCF: 82 +/- 20; total lipid content
In same preliminary experiments it was established that the uptake of the chemicals proceeded very fast, thus enabling the use of this short-term assay.
Description of key information
Deneer, 1987
The bioaccumulation factor (BCF) was determined in an experimental study. Guppies (poecilia reticulata) were exposed to 1,2-dichloro-3-nitrobenzene for 3 days under semi-static conditions in freshwater. The following experimental values were obtained:
BCF: 1027 +/-95 (N=7); whole body w.w.
log BCF: 3.01 +/- 0.04; whole body w.w.
BCF: 82 +/- 20; total lipid content
In same preliminary experiments it was established that the uptake of the chemicals proceeded very fast, thus enabling the use of this short-term assay.
The BCF value obtained from this study (1027, whole body w.w.) is assumed as most conservative value.
Key value for chemical safety assessment
- BCF (aquatic species):
- 1 027
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.