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EC number: 219-524-8 | CAS number: 2454-35-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1999-11-16 to 2000-01-10
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 000
- Report date:
- 2000
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Version / remarks:
- (21 July, 1997)
- Deviations:
- yes
- Remarks:
- E.coli WP2 or S.thyphimurium TA102 were not tested; no historical control data are reported; no cytotoxicity or solubility criteria discussed for the chosen maximum test concentration
- Qualifier:
- according to guideline
- Guideline:
- EPA OPPTS 870.5265 (The Salmonella typhimurium Bacterial Reverse Mutation Test)
- Version / remarks:
- (June 1996)
- Qualifier:
- according to guideline
- Guideline:
- other: EU Method B.14
- Version / remarks:
- (29 December, 1992)
- GLP compliance:
- yes (incl. QA statement)
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- m-acetylphenyl acetate
- EC Number:
- 219-524-8
- EC Name:
- m-acetylphenyl acetate
- Cas Number:
- 2454-35-5
- Molecular formula:
- C10H10O3
- IUPAC Name:
- 3-acetylphenyl acetate
- Test material form:
- solid: particulate/powder
- Remarks:
- migrated information: powder
- Details on test material:
- - Name of test material (as cited in study report): 3-Acetoxyacetophenon ( synonymous to 3-Acetoxyacetophenon lose Ware )
- Physical state: solid
- Analytical purity: 99.0 %
- Purity test date: October 19 , 1999
- Lot/batch No.: 489
- Storage condition of test material: room temperature , light protection
Constituent 1
Method
Species / strainopen allclose all
- Species / strain / cell type:
- S. typhimurium TA 98
- Details on mammalian cell type (if applicable):
- his D 3052; rfa-; uvrB-; R-factor frame shift mutations
- Species / strain / cell type:
- S. typhimurium TA 100
- Details on mammalian cell type (if applicable):
- his G46; rfa-; uvrB-; R-factor base paired subst.
- Metabolic activation:
- with and without
- Metabolic activation system:
- Mammalian microsomal fraction S9 mix
- Test concentrations with justification for top dose:
- 31.6, 100.0, 316.2, 1000.0, 2500.0 and 5000.0 µg/plate
Controlsopen allclose all
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- yes
- Positive controls:
- yes
- Positive control substance:
- sodium azide
- other: 2-aminoanthracene
- Remarks:
- TA 100
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- yes
- Positive controls:
- yes
- Positive control substance:
- other: 4-nitro-o-phenylene-diamine, 2-aminoanthracene
- Remarks:
- TA 98
- Details on test system and experimental conditions:
- METHOD OF APPLICATION: in agar (plate incorporation); preincubation
- Cell density at seeding (if applicable): approx. 10E9 cells/mL
DURATION
- Preincubation period: 60 min at 37°C
- Exposure duration: 48 h
NUMBER OF REPLICATIONS: 3 - Evaluation criteria:
- A test item is considered as mutagenic if:
- a dose-related increase in the number of revertant occurs and/or
- a reproducible biologically relevant positive response for at least one of the test points occurs
in at least one strain with or without metabolic activation.
A biologically relevant increase is described as follows :
- if in strain TA 100 the numbers of reversions is at least twice as high
- if in strain TA 98 the number of reversions is at least three times higher
as compared to the spontaneous rate.
Results and discussion
Test resultsopen allclose all
- Species / strain:
- S. typhimurium TA 98
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity nor precipitates, but tested up to recommended limit concentrations
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity nor precipitates, but tested up to recommended limit concentrations
- Positive controls validity:
- valid
Applicant's summary and conclusion
- Conclusions:
- In conclusion, it can be stated that during the described mutagenicity test under the experimental conditions reported, the test item did not cause
gene mutations by base pair changes or frameshifts in the genome of the tester strains used. - Executive summary:
In order to investigate the potential of 3-Acetoxyacetophenon for its ability to induce gene mutations the plate incorporation test and the pre-incubation test were performed with the Salmonella typhimurium strains TA 98 and TA 100 .
No substantial increase in revertant colony numbers of any of the two tester strains were detected at any dose level of the test item either with or without metabolic activation in both independently performed experiments.
The reported data of this mutagenicity test show that the test item did not induce gene mutations by base pair changes or frameshifts in the genome of the strains used.
Therefore, 3-Acetoxyacetophenon is considered to be non-mutagenic in this bacterial reverse mutation assay.
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