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EC number: 239-405-4 | CAS number: 15373-31-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2015-06-19 to 2015-06-26
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: The study was conducted according to OECD Guideline and GLP.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 015
- Report date:
- 2015
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Version / remarks:
- 1997
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method B.13/14 (Mutagenicity - Reverse Mutation Test Using Bacteria)
- Version / remarks:
- 2008
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- 2,2,3-trimethylcyclopent-3-enylacetonitrile
- EC Number:
- 239-405-4
- EC Name:
- 2,2,3-trimethylcyclopent-3-enylacetonitrile
- Cas Number:
- 15373-31-6
- Molecular formula:
- C10H15N
- IUPAC Name:
- 2-(2,2,3-trimethylcyclopent-3-en-1-yl)acetonitrile
Constituent 1
Method
- Target gene:
- Salmonella typhimurium: histidine locus
Escherichia coli: tryptophan locus
Species / strain
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98, TA 100 and E. coli WP2
- Metabolic activation:
- with and without
- Metabolic activation system:
- Phenobarbital/β-naphthoflavone induced rat liver S9 mix
- Test concentrations with justification for top dose:
- 3; 10; 33; 100; 333; 1000; 2500; and 5000 μg/plate
- Vehicle / solvent:
- - Solvent used: DMSO
- Justification for choice of solvent: The solvent was chosen because of its solubility properties and its relative nontoxicity to the bacteria.
Controlsopen allclose all
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- sodium azide
- Remarks:
- (NaN3), 10 μg/plate, without metabolic activation TA 1535, TA 100
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- other: 4-nitro-o-phenylene-diamine
- Remarks:
- (4-NOPD), without metabolic activation, 10 μg/plate in strain TA 98, 50 μg/plate in strain TA 1537
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- methylmethanesulfonate
- Remarks:
- (MMS), 2.0 μL/plate, without metabolic activation, WP2 uvrA
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- other: 2-aminoanthracene
- Remarks:
- (2-AA), with metabolic activation, TA 1535, TA 1537, TA 98, TA 100, WP2 uvrA
- Details on test system and experimental conditions:
- METHOD OF APPLICATION: in agar (plate incorporation); preincubation
DURATION
- Preincubation period: 60 min
- Exposure duration: 48 h
NUMBER OF REPLICATIONS: 3
DETERMINATION OF CYTOTOXICITY
- Method: reduction in the number of spontaneous revertants or a clearing of the bacterial background lawn - Evaluation criteria:
- A test item was considered as a mutagen if a biologically relevant increase in the number of revertants exceeding the threshold of twice (strains TA 98, TA 100, and WP2 uvrA) or thrice (strains TA 1535 and TA 1537) the colony count of the corresponding solvent control was observed.
A dose dependent increase was considered biologically relevant if the threshold was exceeded at more than one concentration.
An increase exceeding the threshold at only one concentration was judged as biologically relevant if reproduced in an independent second experiment.
A dose dependent increase in the number of revertant colonies below the threshold was regarded as an indication of a mutagenic potential if reproduced in an independent second experiment. However, whenever the colony counts remain within the historical range of negative and solvent controls such an increase was not considered biologically relevant. - Statistics:
- According to the OECD guideline 471, a statistical analysis of the data was not mandatory. Therefore no analysis was performed.
Results and discussion
Test results
- Species / strain:
- S. typhimurium TA 1535, TA 1537, TA 98, TA 100 and E. coli WP2
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Additional information on results:
- TEST-SPECIFIC CONFOUNDING FACTORS
- Precipitation: precipitated in the overlay agar in the test tubes from 2500 to 5000 μg/plate in the presence of S9 mix
ADDITIONAL INFORMATION ON CYTOTOXICITY:
experiment I: without S9: TA1537 (5000 μg/plate), TA100 (2500-5000 μg/plate), WP2uvrA (5000 μg/plate); with S9 mix: TA1535 and TA100 (1000-5000 μg/plate), TA1537 and TA98 and WP2uvrA (2500-5000 μg/plate)
experiment II: seen for all strains with and without S9 mix: 1000-5000 μg/plate - Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Any other information on results incl. tables
Plate incorporation test (experiment 1):
Dose (µg/plate) | Mean number of revertant colonies/3 replicate plates (± S.D.) with different strains of Salmonella typhimurium and E. coli | ||||
TA1535 | TA1537 | TA98 | TA100 | WP2 uvrA | |
Results without S9 | |||||
DMSO | 9 ± 1 | 9 ± 4 | 19 ± 6 | 147 ± 8 | 46 ± 8 |
Untreated | 8 ± 2 | 9 ± 4 | 25 ± 2 | 152 ± 17 | 53 ± 1 |
3 | 10 ± 3 | 9 ± 2 | 21 ± 7 | 166 ± 21 | 49 ± 9 |
10 | 9 ± 4 | 8 ± 2 | 24 ± 7 | 154 ± 7 | 42 ± 6 |
33 | 14 ± 2 | 7 ± 2 | 25 ± 4 | 151 ± 17 | 47 ± 4 |
100 | 8 ± 2 | 7 ± 1 | 26 ± 5 | 150 ± 19 | 47 ± 3 |
333 | 13 ± 4 | 5 ± 0 | 23 ± 5 | 90 ± 19 | 43 ± 6 |
1000 | 15 ± 6 | 7 ± 1 | 17 ± 5 | 67 ± 3 | 33 ± 5 |
2500 | 11 ± 3 | 7 ± 1 | 17 ± 6 | 58 ± 1 | 23 ± 2 |
5000 | 11 ± 5 | 2 ± 2 | 14 ± 2 | 63 ± 10 | 16 ± 3 |
NaN3 (10) | 1205 ± 87 | 2027 ± 45 | |||
4-NOPD (10) | 327 ± 17 | ||||
4-NOPD (50) | 72 ± 12 | ||||
MMS (2.0 µL) | 985 ± 46 | ||||
Results with S9 | |||||
DMSO | 13 ± 1 | 8 ± 0 | 27 ± 5 | 135 ± 1 | 58 ± 8 |
Untreated | 13 ± 4 | 13 ± 3 | 33 ± 2 | 149 ± 4 | 63 ± 12 |
3 | 11 ± 1 | 9 ± 4 | 30 ± 5 | 144 ± 30 | 56 ± 5 |
10 | 11 ± 3 | 11 ± 3 | 27 ± 7 | 134 ± 16 | 60 ± 9 |
33 | 15 ± 2 | 12 ± 2 | 35 ± 3 | 150 ± 20 | 56 ± 6 |
100 | 12 ± 0 | 13 ± 1 | 27 ± 6 | 150 ± 28 | 66 ± 14 |
333 | 8 ± 3 | 15 ± 2 | 35 ± 2 | 110 ± 5 | 61 ± 17 |
1000 | 6 ± 2 | 4 ± 2 | 23 ± 1 | 57 ± 13 | 29 ± 9 |
2500 | 6 ± 2 | 1 ± 1 | 4 ± 1 | 19 ± 3 | 9 ± 3 |
5000 | 1 ± 1 | 0 ± 0 | 0 ± 0 | 0 ± 1 | 1 ± 1 |
2-AA (2.5) | 446 ± 22 | 120 ± 9 | 3437 ± 519 | 3362 ± 163 | |
2-AA (10.0) | 423 ± 41 |
Pre-incubation test (experiment 2:
Dose (µg/plate) | Mean number of revertant colonies/3 replicate plates (± S.D.) with different strains of Salmonella typhimurium and E. coli | ||||
TA1535 | TA1537 | TA98 | TA100 | WP2 uvrA | |
Results without S9 | |||||
DMSO | 15 ± 1 | 10 ± 4 | 20 ± 4 | 118 ± 7 | 43 ± 6 |
Untreated | 11 ± 4 | 10 ± 2 | 21 ± 4 | 148 ± 20 | 37 ± 1 |
3 | 8 ± 3 | 8 ± 2 | 21 ± 2 | 131 ± 10 | 37 ± 4 |
10 | 7 ± 1 | 8 ± 2 | 27 ± 1 | 129 ± 9 | 35 ± 8 |
33 | 11 ± 2 | 8 ± 4 | 18 ± 2 | 124 ± 12 | 41 ± 9 |
100 | 9 ± 2 | 9 ± 2 | 24 ± 3 | 106 ± 29 | 48 ± 8 |
333 | 9 ± 4 | 7 ± 2 | 21 ± 5 | 56 ± 5 | 38 ± 6 |
1000 | 4 ± 2 | 1 ± 1 | 0 ± 1 | 26 ± 23 | 13 ± 7 |
2500 | 2 ± 2 | 0 ± 0 | 0 ± 0 | 11 ± 11 | 11 ± 8 |
5000 | 0 ± 0 | 0 ± 0 | 0 ± 0 | 5 ± 8 | 0 ± 0 |
NaN3 (10) | 1201 ± 21 | 2191 ± 176 | |||
4-NOPD (10) | 338 ± 16 | ||||
4-NOPD (50) | 92 ± 4 | ||||
MMS (2.0 µL) | 608 ± 17 | ||||
Results with S9 | |||||
DMSO | 11 ± 2 | 13 ± 1 | 37 ± 7 | 105 ± 14 | 48 ± 3 |
Untreated | 9 ± 3 | 9 ± 1 | 39 ± 9 | 146 ± 8 | 42 ± 9 |
3 | 8 ± 1 | 11 ± 2 | 32 ± 3 | 101 ± 11 | 43 ± 8 |
10 | 7 ± 2 | 12 ± 5 | 33 ± 1 | 114 ± 5 | 51 ± 5 |
33 | 10 ± 4 | 15 ± 5 | 32 ± 3 | 108 ± 10 | 57 ± 6 |
100 | 13 ± 1 | 17 ± 3 | 36 ± 2 | 108 ± 5 | 54 ± 14 |
333 | 9 ± 4 | 13 ± 3 | 33 ± 4 | 63 ± 8 | 48 ± 1 |
1000 | 0 ± 1 | 1 ± 1 | 1 ± 1 | 2 ± 3 | 21 ± 5 |
2500 | 0 ± 1 | 0 ± 0 | 0 ± 0 | 0 ± 0 | 0 ± 1 |
5000 | 0 ± 0 | 0 ± 0 | 0 ± 0 | 0 ± 0 | 0 ± 0 |
2-AA (2.5) | 420 ± 26 | 134 ± 18 | 4232 ± 148 | 3123 ± 114 | |
2-AA (10.0) | 410 ± 17 |
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
negative
An in vitro reverse mutation assay study (Ames) was conducted in Salmonella typhimurium and Escherichia coli strains. It was concluded that the test substance did not induce gene mutations and was considered to be non-mutagenic. - Executive summary:
An in vitro reverse mutation assay study (Ames) was conducted according to OECD Guideline 471 in Salmonella typhimurium and Escherichia coli strains. Experiment I was performed as a plate incorporation assay. Since a negative result was obtained in this experiment, experiment II was performed as a pre-incubation assay. All tests were conducted in triplicate and concentrations between 3 μg/plate and 5000 μg/plate were tested. Strains were exposed to the test substance with and without metabolic activation with rat liver S9 mix. Cytotoxicity was observed in the plate incorporation test without S9 in three strains (TA1537 (5000 μg/plate), TA100 (2500-5000 μg/plate), WP2uvrA (5000 μg/plate)) and with S9 mix in all strains (TA1535 and TA100 (1000-5000 μg/plate), TA1537 and TA98 and WP2uvrA (2500-5000 μg/plate)). In the pre-incubation test cytotoxicity was observed in all test strains independent of metabolic activation in the range from 1000 to 5000 μg/plate. No substantial increase in revertant colony numbers of any of the five tester strains was detected following treatment with the test substance at any dose level, neither in the presence nor absence of metabolic activation (S9 mix). There was also no tendency of higher mutation rates with increasing concentrations in the range below the generally acknowledged border of biological relevance. Therefore it was concluded that the test substance did not induce gene mutations and it was considered to be non-mutagenic.
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