2-Propenoic acid, heptadecyl ester, branched

Toxicity to soil macroorganisms except arthropods

Link to relevant study record(s)

Reference

Endpoint:

toxicity to soil macroorganisms except arthropods: short-term

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 207 (Earthworm, Acute Toxicity Tests)

GLP compliance:

yes

Analytical monitoring:

no

Vehicle:

yes

Details on preparation and application of test substrate:

- Method of mixing into soil (if used): According to the results of preliminary test, the test substance was not soluble in water, but was soluble in a acetone. The test substance was added into the test system by the test substance solvent stock solution.
An aliquot solvent solution was separately added to about 10g silica sand and the solvent allowed to evaporate off prior to incorporation into formulated artificial soil with deionized reverse osmosis water. These were mixed by a mixer to give the blank control, solvent control, 10, 100 and 1000 mg/kg respectively with a nominal moisture content of 40-60% by mass of the total water-holding capacity with the deionized water. The test medium preparation process of the blank control group was same as the other groups, but without the solvent and the test substance. The test medium preparation process of the solvent control group was same as the other groups, but without the test substance.

Test organisms (species):

Eisenia fetida

Animal group:

annelids

Details on test organisms:

TEST ORGANISM
- Common name: erthworms
- Source: Jiang Su province Jurong Stock Farm. (ID: EF-MKT-170905)
- Age at test initiation (mean and range, SD): adult earthworms
- Weight at test initiation (mean and range, SD): 300 - 600 mg

ACCLIMATION
- Acclimation period: 24 hours
- Acclimation conditions (same as test or not): same

Study type:

laboratory study

Substrate type:

artificial soil

Limit test:

yes

Total exposure duration:

14 d

Test temperature:

19.8°C to 20.2°C

pH:

6.0±0.5

Moisture:

40-60% water content

Details on test conditions:

TEST SYSTEM
- Test container (material, size): 1L breathable glass bottle
- Amount of soil or substrate: 750 g wt (artificial soil)
- No. of organisms per container (treatment): 10
- No. of replicates per treatment group: 4
- No. of replicates per control: 4

SOURCE AND PROPERTIES OF SUBSTRATE (if soil)
- Soil texture (if natural soil)
- 10% w/w Sphagnum peat
- 20% w/w Kaolinite clay (Sigma-Aldrich)
- 70% w/w Silica sand (screened with 0.2 mm sieve)

OTHER TEST CONDITIONS
- Photoperiod: continuous illumination
- Light intensity: 607 - 621 lux

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Mortality after 7 and 14 days

Nominal and measured concentrations:

10, 100 and 1000 mg/kg (range finding)
1000 mg/kg main test

Reference substance (positive control):

yes

Remarks:

chloroacetamide

Duration:

7 d

Dose descriptor:

LC50

Effect conc.:

> 1 000 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Duration:

14 d

Dose descriptor:

LC50

Effect conc.:

> 1 000 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Details on results:

- The mortalities in the blank control group and the solvent control group were both 7.5%. Thus, the test fullfills the validity criterion that control mortality should not exceed 10%.
- No adverse behavioural effects were observed at either 7 or 14 days exposure in any of the treatment groups.

Validity criteria fulfilled:

yes

Conclusions:

Expressed as nominal concentration, the median lethal concentration of C 17 Acrylate on Eisenia fetida are as follows:
The 7 day LC50 value was higher than 1000 mg/kg dry soil weight and the 14 day LC50 value was higher than 1000 mg/kg dry soil weight.

Description of key information

The 7-Day LC₅₀and the 14-Day LC₅₀for the test substance to earthworms (Eisenia fetida) based on the nominal test concentrations were both higher than 1000mg/kg dry soil weight.

Additional information

The acute toxicity of the test substance C 17 Acrylate to Eisenia fetida was determined in a 14 days artificial soil test (OECD Guideline, 207 Earthworm, Acute Toxicity Test). Based on the results of range-finding test, one blank control group, one solvent control group and one treatment group which had four replicates of 10 worms per replicate were included in the limit test. The nominal concentration of the treatment group was1000 mg/kg dry artificial soil weight. The number of mortalities were determined after 7 and 14 days exposure. The mortality of the earthworm in the blank control group was 7.5%, in the solvent control group was 7.5%, which were not more than 10 per cent, so the test was valid. Under the tested conditions, the 7-Day LC₅₀ and the 14-Day LC₅₀ for the test substance to earthworms (Eisenia fetida) based on the nominal test concentrations were both higher than 1000 mg/kg dry soil weight.