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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Link to relevant study record(s)

Description of key information

Fe(Na)EDDHA (CAS 84539-55-9), Daphnia magna, OECD 202: EC50(48h) > 120 mg/L (nominal), EC50(48h) > 122 mg/L (measured) 
Fe(3K)EDDHSA (EC 462-490-6), Daphnia magna, OECD 202: EC50(48h) > 100 mg/L

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates
Effect concentration:
120 mg/L

Additional information

Ecotoxicological effects towards aquatic invertebrates of Fe(3Na)EDDHSA (CAS 84539-54-8) were not investigated experimentally. Read across from the structurally related substances FE(3K)EDDHSA (EC 462 -490 -6) and Fe(Na)EDDHA (CAS 84539-55-9) was performed to fulfil this relevant endpoint under REACH (for further details, please refer to the separate read-across statement).

The acute toxicity of the structurally nearest neighbour Fe(3K)EDDHSA (EC 462-490-6) was investigated concerning the immobilisation ofDaphnia magnaaccording to the principles of OECD Guideline 202 and EU Method C.2 (L´Haridon, 2000). Juvenile daphnids (younger than 24 hour old) were exposed to one concentration of the test substance and compared to a control. Immobilisation of daphnia is determined after an exposure period of 48 hours. The test concentration of the definitive test was 100 mg/L. No immobilisation was observed at this concentration and the measured concentration of the test substance was within +/- 20 % of the nominal value at the beginning and the end of the test. Therefore, the 48-hour EC50 value is considered to be greater than 100 mg/L based on the nominal concentration.

An acute toxicity study investigated the effect of Fe(Na)EDDHA on the immobilisation of Daphnia magna according to the principles of OECD Guideline 202 and EU Method C.2 (Salinas, 2010). Juvenile daphnids (younger than 24 hour old) were exposed to one concentration of the test substance and compared to a control. Immobilisation of daphnia is determined after 24 and 48 hours of exposure. The test substance was tested at 120 mg/L. The 48 hour EC50 value for Fe(Na)EDDHA with Daphnia magna was greater than 120 mg/L based on the nominal concentration of the test substance and greater than 122 mg/L based on the mean measured concentrations. Since the analytically determined concentrations of the test substance in the test solutions were within +/- 20% of the nominal concentrations, the effect concentration is expressed relative to the nominal concentration for the evaluation of the test substance.