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EC number: 203-459-7 | CAS number: 107-07-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- no data
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: The study was performed according to JETOC protocols similar to OECD471. Data from the JETOC is generally well trusted.
Data source
Reference
- Reference Type:
- publication
- Title:
- Mutagenicity Test Data of Existing Chemical Substances
- Author:
- JETOC
- Year:
- 1 996
- Bibliographic source:
- JETOC
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Principles of method if other than guideline:
- The study was performed according to the guidelines of Ministery of Labour, (Japan 1979, 1985 and 1988) and Ames et al. 1975, Maron and Ames (1983) and Matsushima et al. (1980)
- duplicate plates were used instead of triplicate plates but the positive response was confirmed by a repeat experiment. - GLP compliance:
- not specified
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- 2-chloroethanol
- EC Number:
- 203-459-7
- EC Name:
- 2-chloroethanol
- Cas Number:
- 107-07-3
- Molecular formula:
- C2H5ClO
- IUPAC Name:
- 2-chloroethan-1-ol
- Details on test material:
- 2-chloroethanol
Purity: 99.2 %, Wako Pure Chem. Japan
Lot number: AWH5040
Constituent 1
Method
- Target gene:
- histidine operon
Species / strain
- Species / strain / cell type:
- other: S. typhimurium TA 100, TA 1535, TA 98, TA 1537 and E.coli. WP2uvrA
- Metabolic activation:
- with and without
- Metabolic activation system:
- S9 mix
- Test concentrations with justification for top dose:
- 50, 100, 200, 500, 1000, 2000, 5000 µg/plate
- Vehicle / solvent:
- Dimethyl sulfoxide
Controls
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- yes
- Remarks:
- dmso
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- other: see table 1.
- Details on test system and experimental conditions:
- METHOD OF APPLICATION: preincubation
DURATION
- Preincubation period: 20 minutes
- Exposure duration: 48 hours
selection agent: histidine
NUMBER OF REPLICATIONS: duplicate
NUMBER OF CELLS EVALUATED: all colonies are counted
DETERMINATION OF CYTOTOXICITY
- Method: background lawn - Evaluation criteria:
- Chemicals are considered mutagenic when a dose-related increase in revertant colony count is observed and the numer of revertant colonies per plate with the test substance is more than twice that of the negative control (solvent control) and when a reproducibility of the test result is observed.
- Statistics:
- None
Results and discussion
Test resultsopen allclose all
- Species / strain:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not examined
- Positive controls validity:
- valid
- Species / strain:
- E. coli WP2 uvr A
- Metabolic activation:
- with
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not examined
- Positive controls validity:
- valid
- Species / strain:
- E. coli WP2 uvr A
- Metabolic activation:
- without
- Genotoxicity:
- positive
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not examined
- Positive controls validity:
- valid
- Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Any other information on results incl. tables
WP2uvrA positive reponse (average of plates):
µg/plate |
- S9 mix |
|
|
Experiment 1 |
Experiment 2 |
DMSO |
43 |
50 |
50 |
44 |
- |
100 |
33 |
- |
200 |
45 |
- |
500 |
40 |
45 |
1000 |
39 |
50 |
2000 |
65 |
60 |
3000 |
- |
90 |
4000 |
- |
114 |
5000 |
186 |
113 |
AF2 |
553 |
413 |
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
positive
2-chloroathanl was positive in the Ames test under the conditions of this study. WP2uvrA gave a positive response without metabolic activation. This response was confirmed by an independent repeat. All other strains were negative with and without metabolic activation. - Executive summary:
A study was performed according to JETOC protocols similar to OECD471. The study was performed according to the guidelines of Ministery of Labour, (Japan 1979, 1985 and 1988) and Ames et al. 1975, Maron and Ames (1983) and Matsushima et al. (1980). Deviation from th current OECD guideline is that duplicate plates were used instead of triplicate plates (but the positive response was confirmed by a repeat experiment). 50 -5000 µg/plate 2 -chloroethanol, solvent control and positive controls were applied with and without metabolic activation via the preincubation method to TA 100, TA 1535, TA 98, TA 1537 and WP2uvrA.
WP2uvrA gave a positive response without metabolic activation. This response was confirmed by an independent repeat. All other strains were negative with and without metabolic activation.
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