Methyl 3-aminocrotonate

Genetic toxicity: in vitro

Administrative data

Endpoint:

in vitro gene mutation study in bacteria

Remarks:

Type of genotoxicity: gene mutation

Type of information:

experimental study

Adequacy of study:

other information

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Comparable to guideline study with acceptable restrictions

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1986

Report date:

1986

Materials and methods

Principles of method if other than guideline:

The point-mutagenic effects were investigated in doses up to 12000 µg/plate on four histidine-auxotropic Salmonella typhimurium LT2 strains, for each substance, dose level and strain 4 plates were used. After an incubation period of 48 h the number of revertant colonies per plate were counted.

GLP compliance:

no

Type of assay:

bacterial reverse mutation assay

Test material

Test material information

Constituent 1

Reference substance name:

Methyl 3-aminocrotonate

EC Number:

238-056-5

EC Name:

Methyl 3-aminocrotonate

Cas Number:

14205-39-1

Molecular formula:

C5 H9 O2 N

IUPAC Name:

methyl 3-aminocrotonate

Details on test material:

3-Aminocrotonsäuremethylester, white powder, content and identity not certified

Method

Target gene:

histidin gene locus

Species / strain

Species / strain / cell type:

S. typhimurium, other: TA 1535, TA 100, TA 1537, TA 98

Metabolic activation:

with and without

Metabolic activation system:

rat liver homogenate (S-9 mix)

Test concentrations with justification for top dose:

3-Aminocrotonsäuremethylester: 375-12000 µg/plate
Sodium azide: 10 µg/plate
Nitrofurantoin: 0.2 µg/plate
4-Nitro-o-phenylendiamin: 10 µg/plate (TA 1537) and 0.5 µg/plate (TA 98)
2-Aminoanthracene: 3 µg/plate

Controls

Untreated negative controls:

yes

Negative solvent / vehicle controls:

yes

Remarks:

Ethanol

True negative controls:

no

Positive controls:

yes

Positive control substance:

other: Sodium azide (TA 1535, without S9-mix), Nitrofurantoin (TA 100, without S9-mix), 4-Nitro-o-phenylendiamin (TA 1537 and 98, without S9-mix), 2-Aminoanthracene (with S9-mix)

Results and discussion

Test results

Species / strain:

S. typhimurium, other: TA 1535, TA 100, TA 1537, TA 98

Metabolic activation:

with and without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

cytotoxicity

Remarks:

at doses of 1500 µg/plate and higher bacteriotoxic effects, but these were only weak strainspecific effects, so that this range could nevertheless be used for evaluation purposes

Vehicle controls validity:

valid

Untreated negative controls validity:

valid

Positive controls validity:

valid

Remarks on result:

other: all strains/cell types tested

Remarks:

Migrated from field 'Test system'.

Applicant's summary and conclusion

Executive summary:

Methyl 3 -aminocrotonate was tested in the Salmonella/microsome test for point-mutagenic effects in doses up to 12000 µg/plate on the four histidine-auxotrophic Salmonella typhimurium LT2 strains TA 1535, TA 100, TA 1537 and TA 98.

Doses up to and including 750 µg/plate did not cause any bacteriotoxic effects and at higher doses the substance had only a weak strainspecific bacteriotoxic effect, so that this range could nevertheless be used for evaluation purposes.

No evidence for a mutagenic activity of methyl 3 -aminocrotonate was found.