Adenosine 5'-(trihydrogen diphosphate), 2'-(dihydrogen phosphate), 5'→5'-ester with 3-(aminocarbonyl)-1-β-d-ribofuranosylpyridinium hydroxide, inner salt, disodium salt

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Study period:

Juni-11 until Nov-11

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: study conducted under GLP conditions and in acc. with OECD guideline

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2011

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 201 (Alga, Growth Inhibition Test)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Adenosine 5'-(trihydrogen diphosphate), 2'-(dihydrogen phosphate), 5'→5'-ester with 3-(aminocarbonyl)-1-β-d-ribofuranosylpyridinium hydroxide, inner salt, disodium salt

EC Number:

246-129-8

EC Name:

Adenosine 5'-(trihydrogen diphosphate), 2'-(dihydrogen phosphate), 5'→5'-ester with 3-(aminocarbonyl)-1-β-d-ribofuranosylpyridinium hydroxide, inner salt, disodium salt

Cas Number:

24292-60-2

Molecular formula:

C21H28N7O17P3.2Na

IUPAC Name:

Adenosine 5'-(trihydrogen diphosphate), 2'-(dihydrogen phosphate), 5'→5'-ester with 3-(aminocarbonyl)-1-β-d-ribofuranosylpyridinium hydroxide, inner salt, disodium salt

Constituent 2

Reference substance name:

b-NADP, Disodium salt

IUPAC Name:

b-NADP, Disodium salt

Details on test material:

Molecular formula C21H26N7O17P3Na2
Molecular weight 787.4
Description White powder
Expiry date 31 December 2012

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

72 h

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Basis for effect:

growth rate

Effect concentrations 2

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Basis for effect:

biomass

Effect concentrations 3

Duration:

72 h

Dose descriptor:

NOEC

Effect conc.:

100 mg/L

Basis for effect:

growth rate

Effect concentrations 4

Duration:

72 h

Dose descriptor:

NOEC

Effect conc.:

100 mg/L

Basis for effect:

biomass

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Executive summary:

Pseudokirchneriella subcapitata, Fresh Water Algal Growth Inhibitionwith b-NADP, Disodium salt.

The study procedures described in this report were based on the OECD guideline No. 201, 2006. In addition, the procedures were designed to meet the test methods of theCommissionRegulation (EC) No 440/2008, Part C.3, 2008; Amended by EC No. 761/2009and the ISO International Standard 8692, 2004.

The batch of b-NADP, Disodium salt tested was a white powder with a purity of 98% and completely soluble in test medium at the concentrations tested.

A combined limit/range-finding test was performed. Preparation of test solutions started with a concentration of 100 mg/l. No other treatment than vigorous shaking was needed to completely dissolve the test substance. The lower test concentrations were prepared by subsequent dilutions of the highest concentration in test medium. The final test solutions were all clear and colourless.

Six replicates of exponentially growing algal cultures per group were exposed to a control and the concentration of 100 mg/l in the limit test. In addition three replicates per group were exposed to 0.1, 1.0 and 10 mg/l in a range-finding test. The initial cell density was 10⁴cells/ml. The total exposure period was 72 hours and samples for analytical confirmation of the exposure concentrations were taken at the beginning, 24 hours after test initiation and at the end of the test.

The measured concentration in the sample taken from the nominal concentration of 100 mg/l was in agreement with nominal and remained stable during the test.

Based on these results, the effect parameters were expressed in terms of analytically confirmed nominal concentrations.

The study met the acceptability criteria prescribed by the protocol and was considered valid.

No reduction of growth rate or inhibition of yield was recorded at any of the concentrations of b-NADP, Disodium salt tested.

The EC₅₀for growth rate reduction (E_RC₅₀: 0-72h) and the EC₅₀for yield inhibition (E_YC₅₀: 0-72h) were beyond the range tested,

i.e. exceeded an analytically confirmed nominal concentration of 100 mg/l.

The NOEC for growth rate reduction and yield inhibition was 100 mg/l based on analytically confirmed nominal concentrations.