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Diss Factsheets
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EC number: 200-842-0 | CAS number: 75-12-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1989, June 12-16
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Comparable to guideline study with acceptable restrictions (exposure concentrations in the test and the stability of the test item were not confirmed by chemical analysis)
- Qualifier:
- according to guideline
- Guideline:
- other: German Industrial Standard DIN 38412, Part 15
- Deviations:
- yes
- Remarks:
- Test duration 96 h instead of 48 h; compared to nowadays standards the number of test concentrations is low (4 incl. control), but this has no impact on the validity of the study
- GLP compliance:
- no
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: the product was added to the test water without any pretreatment; subsequently the fish were placed into the aquaria - Test organisms (species):
- Leuciscus idus
- Details on test organisms:
- TEST ORGANISM
- Common name: golden orfe
- Strain: golden variety
- Source: Fischzucht Paul Eggers, D/2354 Hohenweststedt
- Length at study initiation (length definition, mean, range and SD): 6.9 cm, range: 6.3-7.5 cm
- Weight at study initiation (mean and range, SD): 2.7 g, range: 2.0-3.6 g
- corpulence factor of the batch: 0.8
- other: During the 3-months housing period, fish were treated twice with 0.05 mg/L malachite green chloride and once with 10 mg/L tetracycline
hydrochloride
ACCLIMATION
- Acclimation period: 3 days
- Feeding frequency: withdrawal of food 1 day before and during exposure
- Health during acclimation (any mortality observed): No
- Test type:
- static
- Water media type:
- freshwater
- Total exposure duration:
- 96 h
- Hardness:
- 2.5 mmol/L
- Test temperature:
- 20°C
- pH:
- 8.0
- Dissolved oxygen:
- > 60%
- Nominal and measured concentrations:
- 0.0 (Control), 2150, 4640, 9300 mg/L (nominal)
- Details on test conditions:
- TEST SYSTEM
- Test vessel: All glass aquaria (30x22x24 cm)
- fill volume: 10 L
- Aeration: Slight with oil free air
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates):
- No. of vessels per control (replicates):
- Biomass loading rate: 2.7 g fish/L test water
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Reconstituted freshwater was prepared from fully demineralized tap water according to DIN 38 412, Part 11
(1982). The water was resalted by the addition of 294.0 mg/L CaCl2*2H2O, 123.3 mg/L MgSO4*7H2O, 63 mg/L NaHCO3 and 5.5 mg/L KCl.
- Ca/mg ratio: 4:1
- acid capacity: 0.8 mmol/L
- Na/K ions = 10:1
- Conductivity:
- Culture medium different from test medium: No
- Intervals of water quality measurement:
OTHER TEST CONDITIONS
- Photoperiod: 16 h light and 8 h darkness
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Determination of mortality and symptoms after 1, 4, 24, 48, 72, 96 h
- Reference substance (positive control):
- yes
- Remarks:
- Chloroacetamide, LC 50 after 48 hours approx. 25 mg/L
- Key result
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 4 640 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- behaviour
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LOEC
- Effect conc.:
- 9 300 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- behaviour
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC0
- Effect conc.:
- 4 640 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- mortality (fish)
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 6 569 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: Geometric mean of LC0 and LC100
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC100
- Effect conc.:
- 9 300 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- mortality (fish)
- Details on results:
- - Other biological observations: Narcotic like state after 24 h, 48 h and 72 h at 9300 mg/L
- Mortality of control: No
- Other adverse effects control: No
- Abnormal responses: tumbling after 48 h at 9300 mg/L - Sublethal observations / clinical signs:
Mortality:
Nominal conc. [mg/L]
Number of dead fish
24 h
48 h
72 h
96 h
Control
0
0
0
0
2150
0
0
0
0
4640
0
0
0
0
9300
0
5
8
10
Physico-chemical parameters:
Nominal concentration
mg/LpH (1 h)
pH (96 h)
Control
7.7
7.5
2150
7.7
7.6
4640
7.6
7.6
9300
7.5
7.8
Oxygen content:
Nominal concentration
mg/LOxygen (1h)
mg/LOxygen (96 h)
mg/LControl
7.9
7.0
2150
8,6
8.0
4640
8.4
8.7
9300
7.8
8.8
- Validity criteria fulfilled:
- not applicable
- Conclusions:
- Since the substance is hydrolytically stable and has no potential for evaporation, the study is acceptable for assessment although the substance concentrations were not verified by analytical monitoring. The LC50 at 96 hours was determined to be 6569 mg/L.
Reference
Description of key information
The LC50/Danio rerio/96 hours was 6562 mg/L and the NOEC was 4640 mg/L in a guideline study demonstrating that the substance is not acutely harmful to fish.
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Dose descriptor:
- LC50
- Effect concentration:
- 6 569 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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