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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2022

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 201 (Freshwater Alga and Cyanobacteria, Growth Inhibition Test)
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Chemical structure
Reference substance name:
2-fluoro-4-methyl-1,3,2-dioxaphospholane
Cas Number:
16415-09-1
Molecular formula:
C3H6FO2P
IUPAC Name:
2-fluoro-4-methyl-1,3,2-dioxaphospholane

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
yes
Details on test solutions:
The required test concentrations were prepared by adding appropriate amount of stock solutions and made up to required volume using OECD alga medium.

Test organisms

Test organisms (species):
Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)

Study design

Test type:
not specified
Water media type:
not specified
Limit test:
yes
Total exposure duration:
72 h

Test conditions

Test temperature:
Temperature of Surrogate(°C):
- Dose Range Finding Study 22.3 to 22.9
- Limit test 22.3 to 22.8
Nominal and measured concentrations:
Nominal concentration: 100.0 mg/L
Details on test conditions:
Light Intensity(Lux):
- Dose Range Finding Study 6001 to 6841
- Limit test 5831 to 6154
Reference substance (positive control):
yes
Remarks:
3, 5 - Dichlorophenol 97%

Results and discussion

Effect concentrationsopen allclose all
Duration:
72 h
Dose descriptor:
EC10
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Duration:
72 h
Dose descriptor:
EC20
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Key result
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Results with reference substance (positive control):
The percent growth rate inhibition as compared with the control is found to be 0.10, 0.28, 0.55, 0.47, 1.10 and 3.08 at the tested concentrations of 0.01, 0.1, 1.0, 10.0, 50.0 and 100.0 mg/L respectively.

The percent growth rate inhibition as compared with the control is found to be 4.66
at the tested concentration of 100.0 mg/L.

The percent inhibition in yield as compared with the control is found to be 0.49, 1.38, 2.76, 2.37, 5.43 and 14.41 at the tested concentrations of 0.01, 0.1, 1.0, 10.0, 50.0 and 100.0 mg/L respectively.

The percent inhibition in yield as compared with the control is found to be 21.54 at the tested concentration of 100.0 mg/L.

Applicant's summary and conclusion

Conclusions:
The toxicity of test item OA-1300 was tested on alga Raphidocelis subcapitata.
The 72 hours EC10, EC20, EC50 values, NOEC and LOEC based on the growth rate and yield was calculated in terms of nominal test item concentration are reported in the following table:

Growth Rate & Yield
Endpoint
(mg/L)
EC10 >100 EC20 >100 EC50 >100
Lower limit >100 Lower limit >100 Lower limit >100
Upper limit >100 Upper limit >100 Upper limit >100
NOEC <100
LOEC <100