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Diss Factsheets

Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2021

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Chemical structure
Reference substance name:
2-fluoro-4-methyl-1,3,2-dioxaphospholane
Cas Number:
16415-09-1
Molecular formula:
C3H6FO2P
IUPAC Name:
2-fluoro-4-methyl-1,3,2-dioxaphospholane

Sampling and analysis

Analytical monitoring:
yes
Details on sampling:
The solubility test in water solubility test, preliminary stability test, definitive test and limited
test was as follows.
- Test solution of water solubility test (Nominal concentration : 100 mg/L)
- Test solution of stability test (Nominal concentration : 100 mg/L)
- Test solution of definitive test {Nominal concentration : (10, 17, 31, 56, 100) mg/L}
- Test solution of limited test {Nominal concentration : 100 (adjust pH) mg/L}
150 mL (water solubility test, stability test) or 440 mL (definitive test, limited test)
was sampled from the middle layer of each test solution, aliquoted from 10 mL,
mixed with 10 mL methanol, and analyzed (three replicates for each step, four
replicates for definitive test). If additional dilution was required, it was diluted with
methanol/water (50/50, v/v %) and analyzed.

Test solutions

Vehicle:
not specified

Test organisms

Test organisms (species):
Daphnia magna

Study design

Test type:
semi-static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
48 h

Test conditions

Hardness:
(140 – 250) mg CaCO3/L
Test temperature:
(18 – 22) ℃, change of water temperature within ±1 ℃
pH:
6.0 – 9.0
Dissolved oxygen:
≧3 mg/L
Nominal and measured concentrations:
In the treatment group without pH adjustment, definitive test was performed at the nominal concentration (10, 17, 31, 56, 100) mg/L {average measured concentration: (2.1, 3.1, 4.1, 5.7, 7.7) mg/L}.
In addition, a limit test was performed in the pH-adjusted treatment group {nominal concentration: 100 mg/L (average measured concentration: 7.4 mg/L)}.

Results and discussion

Effect concentrationsopen allclose all
Key result
Duration:
24 h
Dose descriptor:
EC50
Remarks:
Limited test (after pH adjustment)
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
not specified
Key result
Duration:
48 h
Dose descriptor:
EC50
Remarks:
Limited test (after pH adjustment)
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
not specified
Key result
Duration:
24 h
Dose descriptor:
NOEC
Remarks:
Limited test (after pH adjustment)
Effect conc.:
ca. 100 mg/L
Nominal / measured:
nominal
Conc. based on:
not specified
Key result
Duration:
48 h
Dose descriptor:
NOEC
Remarks:
Limited test (after pH adjustment)
Effect conc.:
ca. 100 mg/L
Nominal / measured:
nominal
Conc. based on:
not specified
Duration:
48 h
Dose descriptor:
NOEC
Remarks:
Definitive test (before pH adjustment)
Effect conc.:
31 mg/L
Nominal / measured:
nominal
Conc. based on:
not specified
Basis for effect:
mobility
Duration:
24 h
Dose descriptor:
NOEC
Remarks:
Definitive test (before pH adjustment)
Effect conc.:
31 mg/L
Nominal / measured:
nominal
Conc. based on:
not specified
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC50
Remarks:
Definitive test (before pH adjustment)
Effect conc.:
55.84 mg/L
95% CI:
>= 48.99 - <= 63.65
Nominal / measured:
nominal
Conc. based on:
not specified
Basis for effect:
mobility
Duration:
24 h
Dose descriptor:
EC50
Remarks:
Definitive test (before pH adjustment)
Effect conc.:
70.58 mg/L
95% CI:
>= 65.24 - <= 76.35
Nominal / measured:
nominal
Conc. based on:
not specified
Basis for effect:
mobility

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The study was conducted to assess the acute toxicity of FCCA-04 under semi-static
system . Determination of EC50 and NOEC were based on nominal concentration and
the measured concentration (Table 6).
9.1 Definitive test (before pH adjustment)
9.1.1 Nominal concentration
- 24 h-EC50 : 70.58 mg/L, [95 % Confidence interval (65.24 – 76.35) mg/L]
- 48 h-EC50 : 55.84 mg/L, [95 % Confidence interval (48.99 – 63.65) mg/L]
- 24 h-NOEC : 31 mg/L
- 48 h-NOEC : 31 mg/L
9.1.2 Measured concentration
- 24 h-EC50 : 6.419 mg/L, [95 % Confidence interval (6.154 – 6.697) mg/L]
- 48 h-EC50 : 5.659 mg/L, [95 % Confidence interval (5.274 – 6.072) mg/L]
- 24 h-NOEC : 4.1 mg/L
- 48 h-NOEC : 4.1 mg/L
9.2 Limited test (after pH adjustment)
9.2.1 Nominal concentration
- 24, 48 h-EC50 : ＞100 mg/L
- 24, 48-NOEC : 100 mg/L
9.2.2 Measured concentration
- 24, 48 h-EC50 : ＞7.4 mg/L
- 24, 48-NOEC : 7.4 mg/L