Absolute of Nicotiana tabacum (Solanaceae) obtained from leaves by organic solvent treatment and subsequent ethanol extraction

Boiling point

Link to relevant study record(s)

Reference

Endpoint:

boiling point

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2019-2020

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 103 (Boiling Point)

Version / remarks:

1995

Deviations:

no

Qualifier:

according to guideline

Guideline:

EU Method A.2 (Boiling Temperature)

Version / remarks:

2008

Deviations:

no

Principles of method if other than guideline:

Not applicable.

GLP compliance:

yes (incl. QA statement)

Remarks:

Inspected on 15-16/11/2017, Signed on 15/05/2018

Type of method:

dynamic method

Key result

Boiling pt.:

> 150 - < 160 °C

Atm. press.:

101 kPa

Discussion and Results

The main determination of the boiling point of the test item was determined with the method following Cottrell.

Two experiments were performed using the Dynamic method using Cottrell apparatus to determine the initial boiling point of the item.

No constant pumping of the Cottrell pump was observed until a temperature maximum of 218 °C (first determination) and 230 °C (second determination) whereas the thermometer in the Cottrell pump showed a much lower temperature than the temperature in the sample 128.6 °C (first determination) and 117.7 °C (second determination).

 

A first rising up in theCottrell pumpcould be observed at 150 °C (first determination) and 160 °C (second determination) whereas the thermometer in the Cottrell pump showed a temperature of 56.9 °C (first determination) and 65.3 °C (second determination).

 

By comparison of all methods results:

Results Boiling Point

Test	Method	Result
Pre-test for boiling point	Siwolloboff	190 °C
Pre-test DSC	DSC Method	weak flat wide behaviour in a range of 166 – 273 °C
Main Tests	Dynamic Method	First rising up into the Cottrel Pump: Temperature Sample: 150- 160 °C Temperature Cottrel Pump: 56.9 – 65.3 °C   Temperature Maximum: Temperature Sample: 218 - 230 °C Temperature Cottrel Pump: 117.7 – 128.6 °C

 

 

The initial boiling point can be supposed to be between 150 and 160 °C, where the first rising up in the Cottrell Pump was observed.

 

Due the UVCB Character and the behavioir of the test item no clear boiling point could be determined under these circumstances.

 

No observations were made which might cause doubts on the validity of the study outcome. Therefore, the result of the study is considered valid.

Conclusions:

The initial boiling point can be supposed to be between 150 and 160 °C.
 

Executive summary:

An experimental study, conducted in accordance with conditions recommended in OECD guideline and under GLP, was performed. The initial boiling point can be supposed to be between 150 and 160 °C, where the first rising up in the Cottrell Pump was observed.

Due the UVCB Character and the behavior of the test item, no clear boiling point could be determined under these circumstances.

No observations were made which might cause doubts on the validity of the study outcome. Therefore, the result of the study is considered valid.

Description of key information

The initial boiling point can be supposed to be between 150 and 160 °C (dynamic method)

Additional information

An experimental study, conducted in accordance with conditions recommended in OECD guideline and under GLP, was performed.The initial boiling point can be supposed to be between 150 and 160 °C, where the first rising up in the Cottrell Pump was observed.

Due the UVCB Character and the behavior of the test item, no clear boiling point could be determined under these circumstances.

No observations were made which might cause doubts on the validity of the study outcome. Therefore, the result of the study is considered valid.