Absolute of Nicotiana tabacum (Solanaceae) obtained from leaves by organic solvent treatment and subsequent ethanol extraction

Density

Link to relevant study record(s)

Reference

Endpoint:

relative density

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2019-2020

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 109 (Density of Liquids and Solids)

Version / remarks:

2012

Deviations:

no

Qualifier:

according to guideline

Guideline:

EU Method A.3 (Relative Density)

Version / remarks:

2008

Deviations:

no

Principles of method if other than guideline:

Not applicable.

GLP compliance:

yes (incl. QA statement)

Remarks:

Inspected on 15-16/11/2017, Signed on 15/05/2018

Type of method:

pycnometer method

Dynamic viscosity of test material:

The test item was flowable and was injected through a one way syringe.

Key result

Type:

relative density

Density:

0.992 other: no dimension

Temp.:

20 °C

Findings

Pre-test for viscosity

The test item was flowable and it was possible to inject the test item with a one way syringe.

As one of these criteria has been fulfilled the density could have been determined.

Measurement

 

All measured values and the corresponding calculation results are given in the following table:

Measured Values

First Determination
Weighing #	m1	m2	m3
Item	Pycn. empty	Pycn. +H2O	Pycn. + test item
Mass in g	30.9030	40.8285	40.7559
Temperature in^°C	20.2	20.2	20.2
Second Determination
Weighing #	m1	m2	m3
Item	Pycn. empty	Pycn. +H2O	Pycn. + test item
Mass in g	30.9006	40.8229	40.7748
Temperature in^°C	20.2	20.2	20.4

Pycn. = Pycnometer

 

Calculated Values

First Determination			Error rel.	Error abs.
Temperature	20.2	°C		0.1
Density of water	0.9981652	g/cm^3	2.00%	0.0002
Volume of Pycnometer	9.9437	cm^3
Density of Test Item	0.9909	g/cm3	0.0121%	0.0001
Second Determination			Error rel.	Error abs.
Temperature	20.2	°C		0.1
Density of water	0.9981652	g/cm^3	2.00%	0.0002
Volume of Pycnometer	9.9405	cm^3
Density of Test Item	0.9933	g/cm^3	0.0121%	0.0001

rel. =relative; abs. = absolute

Presentation of Results

Results of the single determinations with the respective error as well as the mean value and its standard deviation are presented in the following tables:

Results of Single Determinations

Determination	Density in g/cm^3	Error of Determ. in g/cm^3
1	0.9909	0.0001
2	0.9933	0.0001

 

Results – Mean Values

Parameter	Value	Unit	Standard Deviation	Relative Standard Deviation
Volume of Pycnometer	9.94	cm^3	0.0023	0.02 %
Density of Test Item	0.9921	g/cm^3	0.0017	0.18 %
Density of Test Item	992.1	kg/m^3	1.7	0.18 %
Relative Density of Test Item	0.9921		0.0017	-

Note: All calculations are performed with unrounded values. Therefore, re-calculation with rounded values maylead to slightly different results.

Conclusions:

Using the pycnometer method, a density of 0.9921 ± 0.0001 g/cm3 at 20.0 ± 0.4 °C was determined.

Executive summary:

An experimental study, conducted in accordance with conditions recommended in OECD guideline and under GLP, is performed. The density of the test itemwas calculated as 0.9921 ± 0.0001 g/cm³at 20.0 ± 0.4°C, which is the mean of two values. Precision of the value is given as maximal error of determination.

Standard deviation shows very good correlation of the two determinations.

Therefore, the study is considered valid.

Description of key information

Using the pycnometer method, a density of 0.9921 ± 0.0001 g/cm3 at 20.0 ± 0.4 °C was determined.

Key value for chemical safety assessment

Relative density at 20C:

0.992

Additional information

An experimental study, conducted in accordance with conditions recommended in OECD guideline and under GLP, is perfomed. It should be considered as a key study. The result should be retained as key data.