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EC number: - | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- Sep-Oct 2019
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test (Carbon and Ammonium Oxidation))
- Version / remarks:
- OECD 209 resp. EU C.11
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- The test item was stored in the test facility in a closed vessel dry at room temperature (20 ± 5 °C).
- Analytical monitoring:
- not required
- Vehicle:
- no
- Details on test solutions:
- A stock solution containing 10.0044 g/L in deionised water was prepared. The stock solution then was used to prepare the treatments.
- Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- Activated sludge from a biologic sewage treatment plant was used. The chosen plant treats mostly domestic sewage.
The sludge was taken from the activation basin of the ESN (Stadtentsorgung Neustadt) sewage treatment plant in D-67435 NW-Lachen-Speyerdorf, Im Altenschemel.
Upon arrival in the test facility, the sludge was filtrated, washed with tap water 3 times and re-suspended in tap water. The activated sludge was aerated until usage in the test and fed daily with 50 mL synthetic sewage feed /L. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Test temperature:
- 18.9 – 21.3 °C
- pH:
- 7.8-8
- Nominal and measured concentrations:
- Vessel No. Content Concentration in mg/L
(nominal)
1 Blank Control 0
2 Blank Control 0
3 Positive Control 5
4 Positive Control 10
5 Positive Control 20
6 Positive Control 40
7 Blank Control 0
8 Blank Control 0
9 Test Item 1000
10 Test Item 1000
11 Test Item 1000
12 Test Item 1000
13 Test Item 1000
14 Test Item 100
15 Test Item 10
16 Test Item 1
17 Blank Control 0
18 Blank Control 0 - Details on test conditions:
- Duration 3 hours
Replicates
Positive Control 1 replicate/concentration
Test item 1 replicate/concentration (lower concentrations) 5 replicates/concentration (highest concentration)
Blank Control 2 replicates before and 2 after measuring positive control and test item, respectively
Water tap water
Aeration purified air, using Pasteur pipettes,
Feeding nutrient solution, 16 mL/vessel
Temperature 18.9 – 21.3 °C - Reference substance (positive control):
- yes
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Key result
- Duration:
- 3 h
- Dose descriptor:
- NOEC
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Details on results:
- One experiment was performed.
The test item was tested using 4 concentrations ranging from 1000 to 1 mg/L nominal con-centration. For the treatment 1000 mg/L, 5 replicates were used; for the other treatments, 1 replicate each.
Because no significant inhibition was observed, no additional experiment had to be per-formed.
All validity criteria were met. For the estimation of the EC50 of the positive control, the fits showed good statistical correspondence of the data with the dose-response-equation. The positive control gave an EC50 of 9.6 mg/L (95 % confidence interval 5.0 – 14 mg/L) which lie within the recommended range of 2 – 25 mg/L. The coefficient of variation of oxygen uptake rate in control replicates was below 30% at the end of the test. The oxygen uptake rate of the blank controls was above 20 mg O2 per gram activated sludge in 1 hour.
No inconsistencies in the dose-response estimation could be observed. Therefore, no fur-ther experiment was performed in order to discern between inhibition of nitrificators and inhibition of total population.
No observations were made which might cause doubts concerning the validity of the study outcome.
The result of the test can be considered valid - Results with reference substance (positive control):
- The calculated values for the positive control 3,5-Dichlorophenol are presented in the fol-lowing table.
Table 8.1-a 3h-EC50 Positive Control
Date of Experiment 3h-EC50 95% Confidence Interval
08. Oct. 2019 9.6 5.0 – 14 mg/L
The value lies within the recommended range of 2 – 25 mg/L. - Validity criteria fulfilled:
- yes
- Conclusions:
- One valid experiment was performed.
The test item was tested using 4 concentrations ranging from 1000 to 1 mg/L nominal con-centration. Duration of the test was 3 hours. Activated sludge was used as inoculum. It was taken from a domestic sewage treatment plant and washed before usage. The dry matter was determined as 3.1 g suspended solids/L, giving a concentration of 1.55 g suspended solids/L in the test.
3,5-Dichlorophenol was used as positive control. Four concentrations were tested; an EC50 of 9.6 mg/L (95% confidence interval: 5.0 – 14 mg/L) was determined, which lies within the recommended range of 2 – 25 mg/L stated in the OECD guideline.
Because no significant inhibition was observed, no additional experiment had to be per-formed
The following results for the test item E17-194T were determined:
3h NOEC = 1000 mg/L
3h EC10 > 1000 mg/L
3h EC50 > 1000 mg/L - Executive summary:
The following results for the test item E17-194T were determined:
3h NOEC = 1000 mg/L
3h EC10 > 1000 mg/L
3h EC50 > 1000 mg/L
Reference
Description of key information
The test item was tested using 4 concentrations ranging from 1000 to 1 mg/L nominal con-centration. Duration of the test was 3 hours. Activated sludge was used as inoculum. It was taken from a domestic sewage treatment plant and washed before usage. The dry matter was determined as 3.1 g suspended solids/L, giving a concentration of 1.55 g suspended solids/L in the test.
3,5-Dichlorophenol was used as positive control.
Key value for chemical safety assessment
- EC50 for microorganisms:
- 1 000 mg/L
- EC10 or NOEC for microorganisms:
- 1 000 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.