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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
comparable to guideline study
Reason / purpose:
reference to same study
Qualifier:
according to
Guideline:
other: Annex V to EU Directive 79/831/EEC (4) , C2 method
Qualifier:
equivalent or similar to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
GLP compliance:
no
Analytical monitoring:
no
Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The test medium of the highest test concentration of nominal 500 mg/l was prepared. Subsequently the stock solution was diluted serially with test water to obtain the desired test concentrations.
Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Source: originally obtained from the Institute National de Recherche Chimique Appliquee, France and cultured in the laboratories of BASF since 1978.
- Age at study initiation (mean and range, SD): 2-24 hours

ACCLIMATION
- Type and amount of food: Green alga culture once daily
- Feeding frequency: daily
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Hardness:
2 .70 ± 0 .5 mmol/I
Test temperature:
19 ± 1 °C
pH:
6.31 - 8.15
Dissolved oxygen:
8.61 - 9.25 mg/l
Nominal and measured concentrations:
Nominal test substance concentrations:
0, 31.25, 62.5, 125, 250, 500 mg/l
Details on test conditions:
TEST SYSTEM
- Test vessel:flat bottom glasses
- Fill volume: 10 ml
- No. of organisms per vessel:5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water:

- Ca/mg ratio: 4:1
- Conductivity: 600-700 µSiemens/cm


OTHER TEST CONDITIONS
- Photoperiod: 16:8 h day-night regime
- Light intensity: About 5 microEinstein (m²s) in the wavelength range
of 400-750 nm

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Mobility after 3, 6, 24 and 48 hours
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
410 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Details on results:
48 h NOEC 250 mg/L (nominal) based on test material
48 h EC0 250 mg/L (nominal) based on test material
48 h EC100 >=500 mg/L (nominal) based on test material
Conclusions:
The EC50 of the short term toxicity on the mobility of Daphnids was determined to be 410 mg/L.

Description of key information

With high probability acutely not harmful to aquatic invertebrates.

Key value for chemical safety assessment

EC50/LC50 for freshwater invertebrates:
410 mg/L

Additional information

The acute toxicity of the test substance to Daphnia magna was examined in a static test conducted according to EU Directive 79/831/EEC. Test organisms of the species Daphnia magna were exposed to the substance for 48 h. At test termination, an EC50 of 410 mg/L (nominal) was determined.