[2-(methacryloyloxy)ethyl]trimethylammonium methyl sulphate

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1994

Reliability:

1 (reliable without restriction)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1994

Report date:

1994

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

[2-(methacryloyloxy)ethyl]trimethylammonium methyl sulphate

EC Number:

229-995-1

EC Name:

[2-(methacryloyloxy)ethyl]trimethylammonium methyl sulphate

Cas Number:

6891-44-7

Molecular formula:

C9H18NO2.CH3O4S

IUPAC Name:

[2-(methacryloyloxy)ethyl]trimethylammonium methyl sulphate

Constituent 2

Reference substance name:

Ethanaminium, N,N,N-trimethyl-2-[(2-methyl-1-oxo-2-propenyl)oxy]-, methyl sulfate

IUPAC Name:

Ethanaminium, N,N,N-trimethyl-2-[(2-methyl-1-oxo-2-propenyl)oxy]-, methyl sulfate

Details on test material:

- Name of test material (as cited in study report): MADAMDMS
- Physical state: Slightly viscous and colourless liquid
- Analytical purity: no data
- Composition of test material, percentage of components: 80% solution in water
- Lot/batch No.: no data
- Storage condition of test material: In darkness and at room temperature.

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

yes

Details on test solutions:

A stock solution (200 mg/l) was prepared approximately one hour before the start of the study, dissolving test substance into dilution water
This stock solution was submitted to manual stirring,

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

Bred in laboratory by acyclic parthenogenesis
control group : 20 Daphnia. , treated groups (3) : 60 Daphnia

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Hardness:

Between 180 and 270 mg of CaC03 per litre .

Test temperature:

21.0°C

pH:

7.62

Dissolved oxygen:

8.3 mg/L

Salinity:

none

Nominal and measured concentrations:

0 (control), 1, 10 and 100 mg

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Type (delete if not applicable): open
- Material, size, headspace, fill volume:
- Renewal rate of test solution (frequency/flow rate):no
- No. of organisms per vessel:0
- No. of vessels per concentration (replicates):1
- No. of vessels per vehicle control (replicates): 1



TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: in mg/L
Ca(N03)2 4 H20= 8, KN03 = 20, MgS04• 7 H20 = 6, K2HP04= 8, CuS04• 5 H20 = 0.003, (NH4)6 M07 O24, 4 H2O = 0.006, ZnS04.7 H20 = 0.006
CoCl2 . 6 H20 = 0.006, Mn(N03)2 .4 H20 = 0.006, C6H807 . H20 = 0.006, H3B03 = 0.006,
Ammonium iron III citrate (Fe203 content: 29 - 32%) = 0.1625, FeS04. 7 H20 =0.0625, FeCl3 .6 H20 = 0.0625,
+ Ca(N03)2 . 4 H20 = 415.26, MgCl2 .6H20 = 56.65
1 litre of dilution water contained 200 ml distilled water by the mineral stock solution, and 800 ml natural water (Volvic)

OTHER TEST CONDITIONS
- Adjustment of pH: no


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : immobilisation

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 2

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

ca. 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

No immobilization was observed in the control and test groups
No abnormality was observed in the test media during the study

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

Effective Concentration 48 hours for Daphnia (Daphnia magna) : EC (I) 50 • 48 hours (nominal concentration) = Higher than 100 mg/l.
EC (I) 0 - 48 hours (nominal concentration) = 100 mg/I.

Executive summary:

MADAMDMS was tested to evaluate acute toxicity (48 hours) in Daphnia (Daphnia magna) according toO.E.C.D. Guideline No. 202

The Daphnia were exposed to the test substance at theoretical concentrations of: 1, 10 and 100 mg/l. A control group(i.e.without exposure to any substance) was tested under the same conditions.

Static method, without renewal of medium during the exposure period.

After 24 and 48 hours of exposure, evaluation of the number of Daphnia immobilized at each concentration used and in the control group.

Result:

No immobilization was observed in the control ans tests groups No abnormality was observed in the test media during the study

Effective Concentration 48 hours for Daphnia (Daphnia magna) : EC (I) 50 • 48 hours (nominal concentration) = Higher than 100 mg/l. EC (I) 0 - 48 hours (nominal concentration) = 100 mg/I.