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Environmental fate & pathways

Hydrolysis

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Reference
Endpoint:
hydrolysis
Type of information:
experimental study
Adequacy of study:
key study
Study period:
Not specified
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to
Guideline:
OECD Guideline 111 (Hydrolysis as a Function of pH)
Deviations:
no
GLP compliance:
yes
Radiolabelling:
not specified
Analytical monitoring:
yes
Transformation products:
not specified
Key result
Remarks on result:
not determinable
Remarks:
Due to the lack of a specific analytical method capable of quantifying the test material, the determination of the hydrolysis as a function of pH by OECD 111 was not possible.
Details on results:
Due to the lack of a specific analytical method capable of quantifying the test material, the determination of the hydrolysis as a function of pH by OECD 111 was not possible.
Validity criteria fulfilled:
not applicable
Conclusions:
Due to the lack of a specific analytical method capable of quantifying the test material, the determination of the hydrolysis as a function of pH by OECD 111 was not possible. For this reason, a waiver from OECD 111 testing is requested.
Executive summary:

The test material is a pale yellow solid. During preliminary evaluation of solvent solubility, the test material was found to be insoluble in water and most common organic solvents, with the exception of chloroform and tetrahydrofuran.

OECD 111 requires a specific analytical method for the quantification of the test material as well as potential hydrolysis products in aqueous solution. However, despite the test material having a maximum absorbance at 243 nm, an HPLC method could not be developed as the test material could not be detected using an HPLC-UV system under a variety of conditions.

A method based on LCMSMS was therefore attempted, however not all of the components of the test material could be detected. Additionally, the detected components of the test material separated during chromatography analysis. Due to the lack of precise concentrations of each component in the test material and without the ability to detect each component, an analytical method based on LCMSMS could not be developed.

Due to the lack of a specific analytical method capable of quantifying the test material, a waiver from OECD 111 testing is requested.

Description of key information

Due to the lack of a specific analytical method capable of quantifying the test material, the determination of the hydrolysis as a function of pH by OECD 111 was not possible.

Key value for chemical safety assessment

Additional information

The test material is a pale yellow solid. During preliminary evaluation of solvent solubility, the test material was found to be insoluble in water and most common organic solvents, with the exception of chloroform and tetrahydrofuran.

OECD 111 requires a specific analytical method for the quantification of the test material as well as potential hydrolysis products in aqueous solution. However, despite the test material having a maximum absorbance at 243 nm, an HPLC method could not be developed as the test material could not be detected using an HPLC-UV system under a variety of conditions.

A method based on LCMSMS was therefore attempted, however not all of the components of the test material could be detected. Additionally, the detected components of the test material separated during chromatography analysis. Due to the lack of precise concentrations of each component in the test material and without the ability to detect each component, an analytical method based on LCMSMS could not be developed.

Due to the lack of a specific analytical method capable of quantifying the test material, a waiver from OECD 111 testing is requested.