8,9,10-trinorborna-2,5-diene

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Study period:

February - April 2018

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2018

Report date:

2018

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 201 (Freshwater Alga and Cyanobacteria, Growth Inhibition Test)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

8,9,10-trinorborna-2,5-diene

EC Number:

204-472-0

EC Name:

8,9,10-trinorborna-2,5-diene

Cas Number:

121-46-0

Molecular formula:

C7H8

IUPAC Name:

bicyclo[2.2.1]hepta-2,5-diene

Specific details on test material used for the study:

SOURCE OF TEST MATERIAL
- Source and batch number of test material: Air Liquide Electronics Materials. Batch 1590035136 / 1590035137
- Purity: 100% w/w
- Manufacturing date: August 25, 2017
- Deadline to use : August 16, 2018

STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: Room temperature (20±5°C)
- Stability and homogeneity of the test material and during storage: stable

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

Sampling at the beginning of the test (T = 0h) and at the end of the test (T = 72h).
All the solutions were kept in refrigerator or freeze in order to be analyzed if necessary or relevant.

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)

Study design

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

72 h

Test conditions

Test temperature:

22.5-23.0°C

pH:

7.6-8.1 at the start.
7.9-9.9 after 72h

Nominal and measured concentrations:

Nominal loading rate : 10.0 - 32.0 - 102.30 - 328.0 - 1050.0 mg/L (3 replicates of each)

Results and discussion

Effect concentrations

Effect concentrations 1

Key result

Duration:

72 h

Dose descriptor:

EL50

Effect conc.:

548.9 mg/L

Nominal / measured:

meas. (initial)

Conc. based on:

test mat.

Basis for effect:

growth rate

Effect concentrations 2

Key result

Duration:

72 h

Dose descriptor:

EL50

Effect conc.:

190.7 mg/L

Nominal / measured:

meas. (initial)

Conc. based on:

test mat.

Basis for effect:

other: Yield

Any other information on results incl. tables

Inhibitions of the average specific growth rate in comparison to the Control are presented in the following table:

Nominal test item loading rate		Algae * 10^4 /mL at 0 hour	Specific growth rate µ0-72h	Inhibition rate (% Ir)	Average inhibition of growth rate (%)	Coefficient of variation	Significance
WAF Control	a	1	1.461	Not applicable		1.8	-
	b		1.403
	c		1 406
	d		1 400
	e		1,43
	f		/
WAF 10.0	a	1	1,384	2,6	2,7	Not required	ns
	b		1,339	5,8
	c		1,419	0,1
WAF 32.0	a	1	1,352	4,8	4,3		ns
	b		1,404	1,2
	c		1,318	7,3
WAF 102.0	a	1	1,338	5,9	4,2		ns
	b		1,395	1,8
	c		1,347	5,2
WAF 328.0	a	1	0.842	40,7	36,4		**
	b		1,005	29,3
	c		0,834	41,3
WAF 1050.0	a	1	0,135	90,5	92.7		**
	b		0,135	90,5
	c		0,032	97,8

*see deviation in the report

- : not relevant

ns : not significantly different compared to the Conw Dunnet test a =0.05

**significantly different compared to the Contol, Dunnett test, a=0.05

 

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The two value of BCHD toxicity (EL50) on algae are :
- 548.9 mg/L for the inhibiation of growth rate
- 190.7 mg/L for the yield inhibition

Executive summary:

The aim of this study was to determine the effects of the test item "BCHD (BICYCLO[2.2.1]HEPTA-2,5 DIENE)" on the growth of the unicellular green alga Pseudokirchneriella subcapitata, according to the OECD 201 guideline (March 2006, corrected July 2011). The algae were exposed to Water Accommodated Fractions (WAFs) of the test item, following the "Guidance document on aquatic toxicity testing of difficult substances and mixtures" (OECD No.23).

In order to check the stability of the WAFs in the test solutions, concentration of BCHD in the control and the WAFs was checked by HPLC/DAD analysis at start (t=0h) and at the end of the test (t=72h).For this study, the concentration are expressed in nominal test item "BCHD (BICYCLO[2.2.1]HEPTA-2,5 DIENE)" loading rate.

 

The results of this study are summarized below:

Test item: BCHD

Test system: Pseudokirchneriella subcapitata

Exposure duration: 72 hours

Test concentrations: Control - 10.0 - 32.0 -102.0 - 328.0 and 1050.0 mg/L

Incubator: Temperature 22.5°C — 23.0°C ; shaking at 120 rpm; light intensity: 6308 Lux

Measurement of cell growth: Beckman Coulter Z2 and Malassez counfing cell

 

Validity criteria:

In the control. the increase of the biomass during 72 hours was determined to be higher than factor of 16. The coefficients of variation of daily section-by-section specific growth rate and of average specific growth rate were determined respectively to be less than 35% and 7% in the control.

The validity criteria were successfully fulfilled.

 

Results:

The results obtained for the test " BCHD" on the toxicological endpoint "inhibition of growth rate" indicate an ErL50-(0-72h) = 548.9 mg/L [504.8 — 598.0], based on nominal test item loading rate.

The results obtained for the test item " BCHD" on the toxicological endpoint "Yield inhibition" indicate an EyL50-(0-72h) = 190.7 mg/L [158.7 — 247.9], based on nominal test item loading rate.