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EC number: 204-472-0 | CAS number: 121-46-0
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- April 2018
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 018
- Report date:
- 2018
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- 8,9,10-trinorborna-2,5-diene
- EC Number:
- 204-472-0
- EC Name:
- 8,9,10-trinorborna-2,5-diene
- Cas Number:
- 121-46-0
- Molecular formula:
- C7H8
- IUPAC Name:
- bicyclo[2.2.1]hepta-2,5-diene
Constituent 1
- Specific details on test material used for the study:
- SOURCE OF TEST MATERIAL
- Source and batch number of test material: Air Liquide Electronics Materials. Batch 1590035136 / 1590035137
- Purity: 100% w/w
- Manufacturing date: August 25, 2017
- Deadline to use : August 16, 2018
STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: Room temperature (20±5°C)
- Stability and homogeneity of the test material and during storage: stable
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- Water treatment plant (STEP de Lille)
- Duration of test (contact time):
- 28 d
Initial test substance concentration
- Initial conc.:
- 100 mg/L
- Based on:
- test mat.
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- O2 consumption
Reference substance
- Reference substance:
- benzoic acid, sodium salt
Results and discussion
% Degradation
- Key result
- Parameter:
- % degradation (O2 consumption)
- Value:
- ca. 0.45
- Sampling time:
- 28 d
BOD5 / COD results
BOD5 / CODopen allclose all
- Key result
- Parameter:
- BOD5
- Value:
- ca. 0.01 g O2/g test mat.
- Key result
- Parameter:
- BOD5*100/ThOD
- Value:
- ca. 0.45
- Results with reference substance:
- The reference item "Sodium benzoate" was degraded up to 91.39% and 92.78% (mean value: 92.09%) after 14 days. Therefore, it confirms the suitability of the used aerobic sludge inoculum.
Any other information on results incl. tables
Time | Sodium Benzoate | Tox Control | Test item | ||||
Flask 16 | Flask 17 | Flask 4 | Flask 3 | Flask 5 | Flask 2 | Flask 6 | |
0 | 0,00 | 0,00 | 0,00 | 0,00 | 0,00 | 0,00 | 0,00 |
1 | 26,74 | 25,34 | 8,53 | 7,51 | 0,00 | 0,00 | 0,00 |
2 | 56,27 | 56,27 | 14,68 | 15,02 | 0,00 | 0,00 | 0,00 |
3 | 60,46 | 61,86 | 16,37 | 16,04 | 0,00 | 0,00 | 0,00 |
4 | 67,44 | 68,84 | 18,43 | 18,09 | 0,00 | 0,00 | 0,00 |
5 | 74,53 | 75,92 | 19,79 | 19,79 | 0,00 | 0,00 | 0,00 |
6 | 78,25 | 79,65 | 20,02 | 20,02 | 0,00 | 0,00 | 0,00 |
7 | 83,84 | 82,44 | 20,72 | 20,72 | 0,00 | 0,00 | 0,00 |
8 | 86,73 | 85,33 | 21,40 | 21,40 | 0,00 | 0,00 | 0,00 |
9 | 87,20 | 87,20 | 24,51 | 21,51 | 0,00 | 0,00 | 0,00 |
10 | 89,99 | 88,59 | 24,51 | 21,51 | 0,00 | 0,00 | 0,00 |
11 | 90,92 | 89,52 | 21,74 | 21,74 | 0,00 | 0,00 | 0,00 |
12 | 90,46 | 90,46 | 21,62 | 21,62 | 0,00 | 0,00 | 0,00 |
13 | 91,85 | 91,85 | 21,62 | 21,96 | 0,00 | 0,00 | 0,00 |
14 | 92,78 | 91,39 | 21,85 | 21,85 | 0,00 | 0,00 | 0,00 |
15 | 92,78 | 92,78 | 21,51 | 21,85 | 0,00 | 0,00 | 0,00 |
16 | 95,58 | 94,18 | 22,19 | 22,53 | 0,45 | 0,00 | 0,30 |
17 | 95,58 | 95,58 | 22,19 | 22,87 | 0,45 | 0,45 | 0,45 |
18 | 96,51 | 95,11 | 22,41 | 22,75 | 0,30 | 0,30 | 0,30 |
19 | 96,51 | 95,11 | 22,41 | 22,75 | 0,75 | 0,30 | 0,45 |
20 | 97,44 | 96,04 | 22,30 | 22,64 | 0,60 | 0,15 | 0,30 |
21 | 97,44 | 96,04 | 22,30 | 22,64 | 0,60 | 0,15 | 0,30 |
22 | 97,44 | 96,04 | 22,30 | 22,98 | 0,60 | 0,15 | 0,30 |
23 | 98,44 | 96,04 | 22,64 | 22,98 | 1,04 | 0,15 | 0,45 |
24 | 98,44 | 96,04 | 22,64 | 22,98 | 1,04 | 0,15 | 0,45 |
25 | 97,51 | 96,51 | 22,41 | 22,75 | 0,75 | 0,30 | 0,45 |
26 | 99,04 | 96,04 | 22,30 | 22,64 | 0,60 | 0,15 | 0,30 |
27 | 99,50 | 96,51 | 22,41 | 23,09 | 0,75 | 0,30 | 0,45 |
28 | 99,50 | 96,51 | 22,41 | 23,09 | 0,75 | 0,30 | 0,45 |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- not readily biodegradable
- Conclusions:
- All the validity criteria were successful except the toxicity control, furthermore, the variations between the test item replicates are not applicable due to low values.
According to he OECD guideline 301F, the test item (BCHD) is not considered to be biodegradable. - Executive summary:
This study was carried out in September 21, 2017. There was no deviation and no amendment to the study plan during this study.
The aim of this study was to determine the effects of the test item "BCHD” according to the OECD 301 F guideline, ready biodegradability within an experimental period of 28 days by respirometric method.
The amount of oxygen taken up by the microbial population during the biodegradation of the test item (corrected by blank inoculum, run in parallel) was determined from the change in pressure in apparatus and will be expressed as percentage of theoretical Oxygen Demand (ThOD).
Ready biodegradability of tie test item "BCHD” was performed according to the OECD 301 F guideline.
The degradation rate of the test item "BCHD” reached 0.45%, following ThODNH4, after 28 days of incubation. The 10-Day window failed.
According to the OECD guideline 301F, the test item is not considered to be biodegradable.
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