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EC number: 220-585-8 | CAS number: 2825-82-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Endpoint summary
Administrative data
Description of key information
- Acute oral toxicity: LD50 (rats, hamsters) > 20 mL/kg bw, ca. 18.8 g/kg bw ; LD50(mice) = 3.9 mL/kg bw, ca. 2.8 g/kg bw (No guideline; rel.2, K)
- Acute inhalation toxicity: LC50 (Male rats) = 1221 (1174 -1259) ppm, ca. 6.803 (6.542 - 7.015) mg/L ; LC50 (Female rats) = 1194 (1107 -1287) ppm, ca. 6.653 (6.168 - 7.171) mg/L (No guideline; rel.2, K).
Key value for chemical safety assessment
Acute toxicity: via oral route
Link to relevant study records
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1979
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- data from handbook or collection of data
- Remarks:
- Data on a single study reported in different reports or publications.
- Reason / purpose for cross-reference:
- reference to other study
- Guideline:
- other:
- Principles of method if other than guideline:
- Method not detailed. Five male and five female Fischer 344 rats, five female CS7BL/6 mice and five male Golden syrian hamsters were treated with undiluted test substance at a maximum dose level of 20 mL/kg body weight and observed for 14 days.
- GLP compliance:
- no
- Remarks:
- Pre-GLP but the experiments were conducted according to the "Guide for the Care and Use of Laboratory Animals," Institute of Laboratory Animal Resources, National Research Council.
- Test type:
- standard acute method
- Limit test:
- yes
- Species:
- other: rats, mice and hamsters
- Strain:
- other: rats: Fischer 344; mice: C57BL/6; hamsters: Golden Syrian
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- Not specified
- Route of administration:
- oral: unspecified
- Vehicle:
- unchanged (no vehicle)
- Doses:
- 20 mL/kg bw
- No. of animals per sex per dose:
- rats: 5/sex/dose; mice: 5 females/dose; hamsters: 5 males/dose
- Control animals:
- no
- Details on study design:
- Five male and five female Fischer 344 rats, five female CS7BL/6 mice, five male Golden syrian hamsters; maximum dose of 20 mL/kg body weight; observation for 14 days.
- Key result
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- > 20 mL/kg bw
- Based on:
- test mat.
- Remarks on result:
- other: Rats
- Remarks:
- ca. LD50 > 18.8 g/kg bw
- Sex:
- male
- Dose descriptor:
- LD50
- Effect level:
- > 20 mL/kg bw
- Based on:
- test mat.
- Remarks on result:
- other: Hamsters
- Remarks:
- ca. LD50 > 18.8 g/kg bw
- Sex:
- female
- Dose descriptor:
- LD50
- Effect level:
- ca. 3.9 mL/kg bw
- Based on:
- test mat.
- 95% CL:
- >= 2.2 - <= 6.9
- Remarks on result:
- other: Mice
- Remarks:
- LD50 calculated using the moving average method of Weil. ca. LD50 = 2.8 g/kg bw
- Mortality:
- Partial mortality in rats and hamsters.
In mice, all deaths occurred within 48 hours of dosing. - Clinical signs:
- other: Convulsions were observed immediately preceding death.
- Gross pathology:
- Necropsy of dead revealed congested lungs and GI tract.
- Interpretation of results:
- Category 5 based on GHS criteria
- Conclusions:
- Under the test conditions, the substance is not classified according to the Annex I of the Regulation (EC) No. 1272/2008 (CLP) and classified in Category 5 according to the GHS as the worst-case estimated oral LD50 is between 2000 and 5000 mg/kg bw.
- Executive summary:
In an acute oral toxicity study performed in 1979, five male and five female Fischer 344 rats, five female C57BL/6 mice and five male Golden syrian hamsters were treated with undiluted test material at a dose level of 20 mL/kg bw. Animals were then observed for 14 days.
Deaths observed in rats and hamsters but not enough to determine a LD50 value. In mice, deaths occurred within 48 hours of dosing with convulsions were observed immediately preceding death.
Necropsy of dead revealed congested lungs and GI tract.
In rats and hamsters, oral LD50 > 20 mL/kg bw, which is equivalent to 18.8 g/kg bw.
In female mice, oral LD50 = 3.9 mL/kg bw, which is equivalent to 2.8 g/kg bw.
Under the test conditions, the substance is not classified according to the Annex I of the Regulation (EC) No. 1272/2008 (CLP) and classified in Category 5 according to the GHS as the worst-case estimated oral LD50 is between 2000 and 5000 mg/kg bw.
This study is considered as acceptable and satisfies the requirement for acute oral toxicity endpoint.
Reference
Endpoint conclusion
- Endpoint conclusion:
- no adverse effect observed
- Dose descriptor:
- LD50
- Value:
- 2 800 mg/kg bw
- Quality of whole database:
- The Key study was initially published in 1979 and used in several technical reports and reviews. Compiling the data more or less detailed in different sources, this study was considered sufficiently robust to cover this endpoint.
Acute toxicity: via inhalation route
Link to relevant study records
- Endpoint:
- acute toxicity: inhalation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1979
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- data from handbook or collection of data
- Reason / purpose for cross-reference:
- reference to other study
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 403 (Acute Inhalation Toxicity)
- Principles of method if other than guideline:
- Method not detailed. Six male and six female Fischer 344 rats, and female C57BL/6 mice were exposed to varying concentrations of test material vapor for calculating LC50.
- GLP compliance:
- no
- Remarks:
- Prior to GLP but conducted according to the "Guide for the Care and Use of Laboratory Animals," Institute of Laboratory Animal Resources, National Research Council.
- Test type:
- acute toxic class method
- Limit test:
- no
- Species:
- other: rats and mice (and hamsters)
- Strain:
- other: Fischer 344 rats ; C57BL/6 mice ; Goden Syrian hamsters
- Sex:
- male/female
- Route of administration:
- inhalation: vapour
- Type of inhalation exposure:
- whole body
- Vehicle:
- not specified
- Details on inhalation exposure:
- GENERATION OF TEST ATMOSPHERE / CHAMBER DESCRIPTION
- Exposure apparatus: glass chamber
- Exposure chamber volume: 9 L - Duration of exposure:
- ca. 4 h
- Remarks on duration:
- 6h for hamsters
- Concentrations:
- Rats and hamsters : Not reported ("varying concentrations")
Mice: varying concentrations including 1000 ppm - No. of animals per sex per dose:
- 6 rats/sex. 6 female mice. Not specidied for hamsters.
- Control animals:
- not specified
- Details on study design:
- - Duration of observation period following administration: 14 days
- Frequency of observations and weighing: not reported
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs - Key result
- Sex:
- male
- Dose descriptor:
- LC50
- Effect level:
- ca. 1 221 ppm
- Based on:
- test mat.
- 95% CL:
- ca. 1 174 - ca. 1 259
- Exp. duration:
- 4 h
- Remarks on result:
- other: rats
- Key result
- Sex:
- female
- Dose descriptor:
- LC50
- Effect level:
- ca. 1 194 ppm
- Based on:
- test mat.
- 95% CL:
- ca. 1 107 - ca. 1 287
- Exp. duration:
- 4 h
- Remarks on result:
- other: rats
- Sex:
- female
- Dose descriptor:
- LC50
- Effect level:
- ca. 930 ppm
- Based on:
- test mat.
- Exp. duration:
- 4 h
- Remarks on result:
- other: mice
- Remarks:
- An exact LC50 was not obtained as the difference between zero and 100% mortality was so slight that an attempt to achieve partial mortality was not made. A concentration of 900 ppm resulted in no deaths while 955 ppm caused complete mortality.
- Mortality:
- Yes but no details.
- Clinical signs:
- other: Eye irritation, fine tremors, prostration, ataxia. Death during exposure was preceded by clonic convulsions. Survivors of high concentrations exhibited hind quarter paralysis.
- Interpretation of results:
- Category 3 based on GHS criteria
- Conclusions:
- Under the test conditions, the estimated inhalation LC50 after a 4-hour exposure period were 1221 and 1194 ppm for male and female rats, respectively and 930 ppm for female mice. Therefore, the test substance is classified in Category 3 for acute toxicity by inhalation.
- Executive summary:
In an acute inhalation toxicity study performed in 1979, six male and six female Fischer 344 rats, and female C57BL/6 mice were exposed to varying concentrations of test material during 4 hours. Male Golden Syrian hamsters were exposed to saturated vapor pressure concentrations during 6 hours.
Gross signs of toxicity during exposure included tremors and ataxia.
Death during exposure was preceded by fine tremors, prostration and clonic convulsions. Survivors of high concentrations exhibited hind quarter paralysis which lasted throughout the 14 -day observation period or to time of death, whichever occured first.
No mortality was observed in hamsters.
All of 6 mice exposed to 1000 ppm died within 4 hours.
LC50 (M rats) = 1221 (1174 -1259) ppm, ca. 6803 (6542 - 7015) mg/m3, ca. 6.803 (6.542 - 7.015) mg/L.
LC50 (F rats) = 1194 (1107 -1287) ppm, ca. 6653 (6168 - 7171) mg/m3, ca. 6.653 (6.168 - 7.171) mg/L.
LC50 (F mice) = 930 ppm (Confidence limits could not be calculated), ca. 5182 mg/m3, 5.182 mg/L.
Under the test conditions, the substance is classified in Category 3 for acute toxicity by inhalation according to the Annex I of the Regulation (EC) No. 1272/2008 (CLP) and to the GHS.
This study is considered as acceptable and satisfies the requirement for acute inhalation toxicity endpoint.
Reference
Endpoint conclusion
- Endpoint conclusion:
- adverse effect observed
- Dose descriptor:
- LC50
- Value:
- 6 653 mg/m³ air
- Quality of whole database:
- The Key study was initially published in 1979 and was considered sufficiently robust to cover this endpoint.
Acute toxicity: via dermal route
Endpoint conclusion
- Endpoint conclusion:
- no study available
Additional information
Oral route:
A key study was identified (1979, rel.2) In an acute oral toxicity study performed in 1979 and reused in several official report or review, five male and five female Fischer 344 rats, five female C57BL/6 mice and five male Golden syrian hamsters were treated with undiluted test material at a dose level of 20 mL/kg bw. Animals were then observed for 14 days.
Several deaths observed in rats, hamsters and mice in which all deaths occurred within 48 hours of dosing with convulsions immediately preceding death.
Necropsy of dead revealed congested lungs and GI tract.
In rats and hamsters, oral LD50 > 20 mL/kg bw, which is equivalent to 18.8 g/kg bw.
In femal mice, oral LD50 = 3.9 mL/kg bw, which is equivalent to 2.8 g/kg bw.
Inhalation route:
In an acute inhalation toxicity study performed in 1979, six male and six female Fischer 344 rats, and female C57BL/6 mice were exposed to varying concentrations of test material during 4 hours. Male Golden Syrian hamsters were exposed to saturated vapor pressure concentrations during 6 hours.
Gross signs of toxicity during exposure included tremors and ataxia. Death during exposure was preceded by fine tremors, prostration and clonic convulsions. Survivors of high concentrations exhibited hind quarter paralysis which lasted throughout the 14 -day observation period or to time of death, whichever occured first. No mortality was observed in hamsters. All of 6 mice exposed to 1000 ppm died within 4 hours.
LC50 (M rats) = 1221 (1174 -1259) ppm, ca. 6803 (6542 - 7015) mg/m3, ca. 6.803 (6.542 - 7.015) mg/L
LC50 (F rats) = 1194 (1107 -1287) ppm, ca. 6653 (6168 - 7171) mg/m3, ca. 6.653 (6.168 - 7.171) mg/L
LC50 (F mice) = 930 ppm (Confidence limits could not be calculated), ca. 5182 mg/m3, ca.5.182 mg/L
Justification for classification or non-classification
Harmonized classification:
The substance has no harmonized classification according to the Regulation (EC) No. 1272/2008.
Self classification:
Acute toxicity via Oral route:
Under the test conditions, the substance is not classified according to the criteria of Annex I to the Regulation (EC) No. 1272/2008 (CLP) but classified in Category 5 according to the GHS as the estimated oral LD50 is between 2000 and 5000 mg/kg bw.
Moreover, based on effects observed in the acute oral toxicity study, and its hydrocarbon structure and low viscosity, the test substance should be classified for aspiration hazard (H304) according to the Annex I of the Regulation (EC) No. 1272/2008 (CLP).
Acute toxicity via Dermal route:
No data was available.
Acute toxicity (Inhalation):
Under the test conditions, the substance is classified in Category 3 (H331) according to the criteria of Annex I to the Regulation (EC) No. 1272/2008 (CLP) and to the GHS as the estimated inhalation LC50 is between 2.0 and 10 mg/L.
Specific target organ toxicity: single exposure (Oral):
The classification criteria according to the Annex I of the Regulation (EC) No. 1272/2008 as specific target organ toxicant (STOT) – single exposure, oral are not met since no reversible or irreversible adverse health effects were observed immediately or delayed after exposure and no effects were observed at the guidance value (oral) for a Category 1 classification (C≤ 300 mg/kg bw) and at the guidance value (oral) for a Category 2 classification (2000 mg/kg bw≥C > 300 mg/kg bw). No classification is required.
The criteria for Transient Organ effects (STOT-SE Category 3) according to Annex I of the Regulation (EC) No. 1272/2008 are met since narcotic effects were observed in the acute oral toxicity study. Therefore the substance is classified for STOT-SE 3 by oral route.
Specific target organ toxicity: single exposure (Dermal):
No data was available.
Specific target organ toxicity: single exposure (Inhalation):
The classification criteria according to the Annex I of the Regulation (EC) No. 1272/2008 as specific target organ toxicant (STOT) – single exposure, inhalation are not met since no reversible or irreversible adverse health effects were observed immediately or delayed after exposure and no effects were observed at the guidance value (inhalation, vapour) for a Category 1 classification (C≤ 10 mg/L) and at the guidance value (inhalation, vapour) for a Category 2 classification (20 mg/L≥C > 10 mg/L). No classification is required.
The criteria for Transient Organ effects (STOT-SE Category 3) according to Annex I of the Regulation (EC) No. 1272/2008 are met since narcotic effects were observed in the acute oral toxicity study. Therefore the substance is classified for STOT-SE 3 by inhalation.
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