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Toxicity to microorganisms

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Reference
Endpoint:
activated sludge respiration inhibition testing
Type of information:
experimental study
Adequacy of study:
key study
Study period:
November 10th, 1992
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
test procedure in accordance with generally accepted scientific standards and described in sufficient detail
Qualifier:
according to
Guideline:
OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
Qualifier:
according to
Guideline:
EU Method C.11 (Biodegradation: Activated Sludge Respiration Inhibition Test)
GLP compliance:
yes
Details on test solutions:
Test item was directly added to the testing solution. The test material was weighed directly into the vessels.
Test organisms (species):
activated sludge of a predominantly domestic sewage
Details on inoculum:
- Source: Municipal sewage treatment plant Frankfurt / M-Sindlingen.
- Amount used, activated sludge: 1600 mg/l dry weight.
- Pretreatment: according to the iindications described in the guidelines.
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
3 h
Test temperature:
20 ± 2 °C
pH:
7.2 ± 0.2
Nominal and measured concentrations:
Nominal, in the rage of 500 - 1000 mg/l
Reference substance (positive control):
yes
Remarks:
3,5-Dichlorophenol
Duration:
3 h
Dose descriptor:
EC50
Effect conc.:
> 1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Details on results:
EC20 (3h) > 1000 mg/l
EC80 (3h) > 1000 mg/l
Conclusions:
EC50 (3h) > 1000 mg/l (nominal)
Executive summary:

The respiration inhibition potential on activated sludge, the assay was conducted in accordance with OECD guideline 209 and EU Method C.

Test item was directly added to the testing solution. The test material was weighed directly into the vessels. The tested concentrations renged from 500 to 1000 mg/l. 3,5-Dichlorophenol was used as positive control. The EC20, EC50 and EC80 values, after 3 hours of exposure, resulted to be higher than 1000 mg/l.

Conclusion

EC50 (3h) > 1000 mg/l (nominal)

Description of key information

EC50 (3h) > 1000 mg/l (nominal)

Key value for chemical safety assessment

Additional information

The respiration inhibition potential of Fluorescent Brightener 367 on activated sludge, the assay was conducted in accordance with OECD guideline 209 and EU Method C. Test item was directly added to the testing solution. The test material was weighed directly into the vessels. The tested concentrations renged from 500 to 1000 mg/l and the EC20, EC50 and EC80 values, after 3 hours of exposure, resulted to be higher than 1000 mg/l.