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Short-term toxicity to fish

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Reference
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
April from 03d to 10th, 1981
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment
Remarks:
actual concentrations were not measured
Qualifier:
equivalent or similar to
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
GLP compliance:
not specified
Analytical monitoring:
no
Details on test solutions:
The test compound was insoluble in water and was suspended by means of Ultra-Turrax for 5 minutes at maximum speed in test water; test item was directly added to the test vessels and evenly distributed with a glass rod.
Test organisms (species):
Leuciscus idus
Details on test organisms:
TEST ORGANISM
- Common name: glodorfe.
- Source: Paul Eggers, 2345 Hohenwestedt.
- Length at study initiation: 5.5 - 6.7 cm
- Weight at study initiation: 1.5 - 2.3 g

ACCLIMATION
- Acclimation period: at least 14 days prior to test initiation.
- Acclimation conditions: fish were taken into dechlorinated tap water, at 20 ° C.
- Type of food during acclimation: Tetra Min (Tetra Werke, Melle).
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h
Test temperature:
20 ± 1 °C
pH:
7.9 - 8.3
Dissolved oxygen:
8.2 - 8.9 mg/l
Nominal and measured concentrations:
Nominal 0, 1, 10, 100 and 500 mg/l (total amount of substance introduced into the vessels).
Details on test conditions:
TEST SYSTEM
- Test vessel: the test basins consisted of glass (40 x 25 x 30 cm) and were filled with 20 liters of test water.
- Aeration: approximately 100 ml of air was passed through the basins per minute; the oxygen content was over 7 mg/l.
- No. of organisms per vessel: 10 vessels.
- Biomass loading rate: 10 fish per 20 litres.

TEST MEDIUM / WATER PARAMETERS
- Source of dilution water: the test water used was deionized tap water (CHRIST-Ministil ion exchange cartridge). The following salts were added to water per liter, according to the formulation of MARKING and DAWSON: NaHCO3 192 mg, CaSO4 x 2H2O 120 mg, MgSO4 120 mg, KCl 8 mg.
- Conductivity: < 5 µS/cm
- Total hardness: 9.5 °, 6.4 d as CaCO3.

OTHER TEST CONDITIONS
- Photoperiod: vessels were illuminated from above in a 12-hour day-night rhythm.
- Light intensity: ca 700 Lux.

EFFECT PARAMETERS MEASURED
During the entire trial period, the fish were observed regularly and the behavior recorded. At the end of the experimental period, the fish were killed in 0.05 % MS 222 (methanesulfonate of the meta-amino-benzoic acid ethyl ester, Sandoz), dissected and examined macroscopically.

MEASUREMENTS OF PARAMETERS
Immediately before addition of the substance and after 2, 24, 48, 72 and 96 hours, the parameters pH, O2 and temperature were measured in each test tank.
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
> 500 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Details on results:
In the course of the 96-hour experimental period, no fish died in all the tested concentration groups.
The behavior of the fish of all experimental groups was normal and corresponded that of the controls.
The section of fish killed, after the end of the experiment, showed no macroscopically visible changes.

APPEARANCE OF THE MEDIUM
In the course of the experiment, the test substance concentrated on the water surface and on the bottom of the vessel.
Conclusions:
LD50 (96h) > 500 mg/l (nominal)
Executive summary:

The toxicity of test item on Leuciscus idus was assessed over 96 hours in a static-system. The test compound was insoluble in water and was suspended by means of Ultra-Turrax for 5 minutes at maximum speed in test water; test item was directly added to the test vessels and evenly distributed with a glass rod. The following tested nominal concentrations were assayed: 0, 1, 10, 100 and 500 mg/l (total amount of substance introduced into the vessels). In the course of the experiment, the test substance concentrated on the water surface and on the bottom of the vessel.

In the course of the 96-hour experimental period, no fish died in all the tested concentration groups. The behavior of the fish of all experimental groups was normal and corresponded that of the controls. The section of fish killed, after the end of the experiment, showed no macroscopically visible changes.

Conclusion

LD50 (96h) > 500 mg/l (nominal)

Description of key information

Not harmful/toxic for short-term exposure to fish (LD50 (96h) > 500 mg/l, nominal).

Key value for chemical safety assessment

Additional information

The toxicity of test item on Leuciscus idus was assessed over 96 hours in a static-system. The test compound was insoluble in water and, in the course of the experiment, the test substance concentrated on the water surface and on the bottom of the vessel. The following tested nominal concentrations were assayed: 0, 1, 10, 100 and 500 mg/l (total amount of substance introduced into the vessels). In the course of the 96-hour experimental period, no fish died in all the tested concentration groups. The behavior of the fish of all experimental groups was normal and corresponded that of the controls. The section of fish killed, after the end of the experiment, showed no macroscopically visible changes.