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Short-term toxicity to aquatic invertebrates

Based on the prediction done using the OECD QSAR toolbox version 3.3 with log kow as the primary descriptor and considering the closest read across substances, the toxicity to aquatic invertebrate was predicted 4,4'-[vinylenebis[(3-sulpho-4,1-phenylene)imino(6-chloro-1,3,5-triazine-4,2-diyl)imino]]bis[5-hydroxy-6-[(1-sulpho-2-naphthyl)azo] naphthalene-2,7-disulphonic] acid (CAS: 94022-69-2). EC50 Intoxication value was estimated to be 108.4461 mg/l for Daphnia magna for 48.0 hrs duration. It was concluded that 4,4'-[vinylenebis[(3-sulpho-4,1-phenylene)imino(6-chloro-1,3,5-triazine-4,2-diyl)imino]]bis[5-hydroxy-6-[(1-sulpho-2-naphthyl)azo] naphthalene-2,7-disulphonic] acid (CAS: 94022-69-2) was likely to be not toxic to aquatic invertebrate.

Toxicity to aquatic algae and cyanobacteria

Based on the prediction done using the OECD QSAR toolbox version 3.3 with log kow as the primary descriptor and considering the closest read across substances, the toxicity to aquatic algae was predicted 4,4'-[vinylenebis[(3-sulpho-4,1-phenylene)imino(6-chloro-1,3,5-triazine-4,2-diyl)imino]]bis[5-hydroxy-6-[(1-sulpho-2-naphthyl)azo] naphthalene-2,7-disulphonic] acid (CAS: 94022-69-2). EC50 growth rate value was estimated to be 105.307 mg/l for Pseudokirchneriella subcapitata for 72.0 hrs duration. It was concluded that 4,4'-[vinylenebis[(3-sulpho-4,1-phenylene)imino(6-chloro-1,3,5-triazine-4,2-diyl)imino]]bis[5-hydroxy-6-[(1-sulpho-2-naphthyl)azo] naphthalene-2,7-disulphonic] acid (CAS: 94022-69-2) was likely to be not toxic to aquatic algae.

Thus based on the results of effect concentrations obtained from the predicted data, experimental reports and secondary source for aquatic invertebrate, aquatic algae and cyanobacteria gives the conclusion that test substance

4,4'-[vinylenebis[(3-sulpho-4,1-phenylene)imino(6-chloro-1,3,5-triazine-4,2-diyl)imino]]bis[5-hydroxy-6-[(1-sulpho-2-naphthyl)azo] naphthalene-2,7-disulphonic] acid (CAS: 94022-69-2) is likely to be not toxic to aquatic environment at environmentally relevant concentrations and applying weight of evidence approach it can be considered to be “not classified” as per the CLP classification criteria.

Additional information

Short-term toxicity to aquatic invertebrates

Following studies of target chemical and structurally similar read across includes predicted data and experimental data to conclude the toxicity extent of 4,4'-[vinylenebis[(3-sulpho-4,1-phenylene)imino(6-chloro-1,3,5-triazine-4,2-diyl)imino]]bis[5-hydroxy-6-[(1-sulpho-2-naphthyl)azo] naphthalene-2,7-disulphonic] acid (CAS: 94022-69-2) towards aquatic invertebrate is summarized as follows:

Based on the prediction done using the OECD QSAR toolbox version 3.3 with log kow as the primary descriptor and considering the closest read across substances, the toxicity to aquatic invertebrate was predicted 4,4'-[vinylenebis[(3-sulpho-4,1-phenylene)imino(6-chloro-1,3,5-triazine-4,2-diyl)imino]]bis[5-hydroxy-6-[(1-sulpho-2-naphthyl)azo] naphthalene-2,7-disulphonic] acid (CAS: 94022-69-2). EC50 Intoxication value was estimated to be 108.4461 mg/l for Daphnia magna for 48.0 hrs duration. It was concluded that 4,4'-[vinylenebis[(3-sulpho-4,1-phenylene)imino(6-chloro-1,3,5-triazine-4,2-diyl)imino]]bis[5-hydroxy-6-[(1-sulpho-2-naphthyl)azo] naphthalene-2,7-disulphonic] acid (CAS: 94022-69-2) was likely to be not toxic to aquatic invertebrate.

The above predicted data of target chemical is supported by the experimental study of structurally similar read across trisodium 5-({4-chloro-6-[ethyl(phenyl)amino]-1,3,5-triazin-2-yl}amino)-4-hydroxy-3-[(1-sulfonato-2-naphthyl)diazenyl]naphthalene-2,7-disulfonate (Cas no. 84045-65-8) from the experimental ABITEC Lab report 2017, suggests that the Experimental study for inhibition of the mobility of daphnids was carried out with the trisodium 5-({4-chloro-6-[ethyl(phenyl)amino]-1,3,5-triazin-2-yl}amino)-4-hydroxy-3-[(1-sulfonato-2-naphthyl)diazenyl]naphthalene-2,7-disulfonate according to OECD Guideline 202 (Report no. 174/17, Abitec 2017). The animals used for the test shall be less than 24 h old and should not be of first brood progeny. Test solutions of required concentration were prepared by mixing the stock solution of the test sample with reconstituted test water. The test substance was tested at the concentration of 0,30,45,67,100 and 150 mg/l. The test was performed under static conditions in fresh water system at 20±1°C temperature. Effects on immobilisation were observed for 48 hours. EC50 was calculated using non linear regression by the software Prism 4.0.The median effective concentration (EC50) for the test substance trisodium 5-({4-chloro-6-[ethyl(phenyl)amino]-1,3,5-triazin-2-yl}amino)-4-hydroxy-3-[(1-sulfonato-2-naphthyl)diazenyl]naphthalene-2,7-disulfonate (Cas no. 84045-65-8) in Daphnia magna was determined to be 103.8 mg/L for immobilisation effects.

Based on the value trisodium 5-({4-chloro-6-[ethyl(phenyl)amino]-1,3,5-triazin-2-yl}amino)-4-hydroxy-3-[(1-sulfonato-2-naphthyl)diazenyl]naphthalene-2,7-disulfonate was considered to be non toxic to aquatic invertebrates and thus "not classified" as per the CLP regulation.

Another supporting experimental study for the structurally similar read across suramin (CAS: 145-63-1) from the secondary source 1993, also suggests As in vitro experiment was performed on 8 Gyrodactvlus (worm) were taken from fish and placed in petri dish with added suramin and observed for 30 and 60 min after exposing to the suramin. A control trial was also used were zero mortality observed. After exposure period only 1 worm death was observed.On the basis of this, the LD 50 value for aquatic invertebrates for suramin (145-63-1) was determined to be greater than 200 mg/l as 1 worm was died out of 8 used for experiment. Based on the result , the suramin was considered as "not classified" according to CLP regulations.

Thus based on the effect concentrations which is in the range 103.8 mg/L to200 mg/lgive the conclusion that test substance 4,4'-[vinylenebis[(3-sulpho-4,1-phenylene)imino(6-chloro-1,3,5-triazine-4,2-diyl)imino]]bis[5-hydroxy-6-[(1-sulpho-2-naphthyl)azo] naphthalene-2,7-disulphonic] acid (CAS: 94022-69-2) is likely to be not toxic to aquatic environment at environmentally relevant concentrations and applying weight of evidence approach it can be considered to be “not classified” as per the CLP classification criteria.

Toxicity to aquatic algae and cyanobacteria

Following studies of target chemical and structurally similar read across includes predicted data and experimental data to conclude the toxicity extent of 4,4'-[vinylenebis[(3-sulpho-4,1-phenylene)imino(6-chloro-1,3,5-triazine-4,2-diyl)imino]]bis[5-hydroxy-6-[(1-sulpho-2-naphthyl)azo] naphthalene-2,7-disulphonic] acid (CAS: 94022-69-2) towards aquatic algae is summarized as follows:

Based on the prediction done using the OECD QSAR toolbox version 3.3 with log kow as the primary descriptor and considering the closest read across substances, the toxicity to aquatic algae was predicted 4,4'-[vinylenebis[(3-sulpho-4,1-phenylene)imino(6-chloro-1,3,5-triazine-4,2-diyl)imino]]bis[5-hydroxy-6-[(1-sulpho-2-naphthyl)azo] naphthalene-2,7-disulphonic] acid (CAS: 94022-69-2). EC50 growth rate value was estimated to be 105.307 mg/l for Pseudokirchneriella subcapitata for 72.0 hrs duration. It was concluded that 4,4'-[vinylenebis[(3-sulpho-4,1-phenylene)imino(6-chloro-1,3,5-triazine-4,2-diyl)imino]]bis[5-hydroxy-6-[(1-sulpho-2-naphthyl)azo] naphthalene-2,7-disulphonic] acid (CAS: 94022-69-2) was likely to be not toxic to aquatic algae.

The above predicted data of target chemical is supported by the experimental study of structurally similar read across trisodium 5-({4-chloro-6-[ethyl(phenyl)amino]-1,3,5-triazin-2-yl}amino)-4-hydroxy-3-[(1-sulfonato-2-naphthyl)diazenyl]naphthalene-2,7-disulfonate (Cas no. 84045-65-8) from the experimental ABITEC Lab report 2017, suggests that theFreshwater algal growth inhibition test was carried out on Desmodesmus subspicatus with the substance trisodium 5-({4-chloro-6-[ethyl(phenyl)amino]-1,3,5-triazin-2-yl}amino)-4-hydroxy-3-[(1-sulfonato-2-naphthyl)diazenyl]naphthalene-2,7-disulfonate (CAS no. 84045-65-8) to OECD Guideline 201. The stock solution (220 mg/L) was prepared by dissolving dark brown powder in OECD growth medium.Test solutions  of required concentrations were prepared by mixing the stock solution of the test sample with OECD growth medium and inoculum culture. Conc. of test chemical used for the study was 0, 77, 100, 130, 170 and 220 mg/L, respectively. The test was performed under static conditions in a static fresh water system at a temp. of 23±2°C. Initial cell density of test organism used was 5x10(3) cells/ml. Determination of cell counting involve the use of microscope with counting chamber Cyrus I or electronic particle counter. ErC50 was calculated using non-linear regression by the software Prism 4.0. The median effective concentration (EC50) for the test substance trisodium 5-({4-chloro-6-[ethyl(phenyl)amino]-1,3,5-triazin-2-yl}amino)-4-hydroxy-3-[(1-sulfonato-2-naphthyl)diazenyl]naphthalene-2,7-disulfonate in Desmodesmus subspicatus was determined to be 656.3 mg/L on the basis of effects on growth rate in a 72 hour study. Thus, based on this value, test chemical can be considered as non toxic to aquatic organisms and thus can be 'not classified' in aquatic compartment as per the CLP criteria.

Thus based on the effect concentrations which is in the range 105.307 mg/L to656.3 mg/lgive the conclusion that test substance 4,4'-[vinylenebis[(3-sulpho-4,1-phenylene)imino(6-chloro-1,3,5-triazine-4,2-diyl)imino]]bis[5-hydroxy-6-[(1-sulpho-2-naphthyl)azo] naphthalene-2,7-disulphonic] acid (CAS: 94022-69-2) is likely to be not toxic to aquatic environment at environmentally relevant concentrations and applying weight of evidence approach it can be considered to be “not classified” as per the CLP classification criteria.

Thus based on the results of effect concentrations obtained from the predicted data, experimental reports and secondary source for aquatic invertebrate, aquatic algae and cyanobacteria gives the conclusion that test substance

4,4'-[vinylenebis[(3-sulpho-4,1-phenylene)imino(6-chloro-1,3,5-triazine-4,2-diyl)imino]]bis[5-hydroxy-6-[(1-sulpho-2-naphthyl)azo] naphthalene-2,7-disulphonic] acid (CAS: 94022-69-2)is likely to be not toxic to aquatic environment at environmentally relevant concentrations and applying weight of evidence approach it can be considered to be “not classified” as per the CLP classification criteria