3-nitro-o-xylene

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Test procedure in accordance with national standard methods, without detailed documentation, but basic data given

Data source

Reference

Reference 1

Reference Type:

study report

Title:

Unnamed

Year:

1986

Reference 2

Reference Type:

publication

Title:

A QSAR study of the toxicity of nitrobenzene derivatives towards Daphnia magna, Chlorella pyrenoidosa and Photobacterium phosphoreum

Author:

Deneer JW, van Leeuwen CJ, Seinen W, Maas-Diepeveen JL, Hermens JLM

Year:

1989

Bibliographic source:

Aquatic Toxicology 15: 83-98

Materials and methods

Principles of method if other than guideline:

other: NEN 6501: Determination of acute toxicity with Daphnia magna (1980) with slight modifications (Van Leeuwen et al. 1985)

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

3-nitro-o-xylene

EC Number:

201-474-3

EC Name:

3-nitro-o-xylene

Cas Number:

83-41-0

Molecular formula:

C8H9NO2

IUPAC Name:

1,2-dimethyl-3-nitrobenzene

Test material form:

liquid

Details on test material:

3-Nitro-o-xylene
pure (not specified)

Sampling and analysis

Analytical monitoring:

no

Test organisms

Test organisms (species):

Daphnia magna

Study design

Test type:

static

Water media type:

freshwater

Total exposure duration:

48 h

Test conditions

Hardness:

200 mg/l CaCO3

Test temperature:

20 ± 0.5 °C

pH:

8.4 ± 0.1

Dissolved oxygen:

medium was saturated with air prior to use

Details on test conditions:

- During the tests daphnids were fed with Chlorella pyrenoidosa, which at the start of the experiments were present at a concentration of 1.0E+8 cells/L
- test was carried out in a room that was illuminated 12 h/day
- a synthetic test medium was used (NPR 6503,1980)

Results and discussion

Effect concentrations

Duration:

48 h

Dose descriptor:

IC50

Effect conc.:

4.2 mg/L

Nominal / measured:

not specified

Conc. based on:

not specified

Basis for effect:

mobility

Remarks on result:

other: 95% CL: (3.2-5.6)

Reported statistics and error estimates:

Maas-Diepeveen and van Leeuwen (1986) calculated LC-50 48-h values and their 95 % confidence intervals according to Litchfield and Wilcoxon (1949).