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Environmental fate & pathways

Bioaccumulation: aquatic / sediment

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Reference
Endpoint:
bioaccumulation in aquatic species: fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2005-09-20 to 2005-12-05
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Reference:
Composition 0
Qualifier:
according to
Guideline:
other: METI Guideline: Concentration Test on Chemical Substances in Fish
Deviations:
no
Qualifier:
according to
Guideline:
OECD Guideline 305 (Bioconcentration: Flow-through Fish Test)
Deviations:
no
GLP compliance:
yes (incl. certificate)
Test material information:
Composition 1
Specific details on test material used for the study:
Batch: S000153

Radiolabelling:
no
Details on sampling:
- Sampling intervals/frequency for test organisms: days 0, 7, 14, 21, 28
- Sampling intervals/frequency for test medium samples: days 0, 7, 14, 21, 28
- Fish: two fish per sampling homogenised, and extracted with acetonitril/n-hexane. Further analysis performed by HPLC and GC.
Vehicle:
yes
Details on preparation of test solutions, spiked fish food or sediment:
According to guideline
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): HCO-40
- Concentration of vehicle in test medium (stock solution and final test solution(s) at different concentrations and in control(s)): 5 g/l
Dose 1: Test item: 0.2 mg/L , HCO-40: 10 g/L
Dose 2: Test item: 0.02 mg/L, HCO-40: 10 g/L
Control: Test item: --, HCO-40: 10 g/L
Test organisms (species):
Cyprinus carpio
Details on test organisms:
Weight: 6.6 g
Length: 8.3 cm
Lot: 050714c
Route of exposure:
aqueous
Test type:
flow-through
Water / sediment media type:
natural water: freshwater
Total exposure / uptake duration:
28 d
Total depuration duration:
0
Test temperature:
24.6 - 25.0 °C
pH:
7.3-7.4
Dissolved oxygen:
saturated
Salinity:
According to guideline
Details on test conditions:
After acclimatisation period, the fish (Cyprinus carpio) were transferred into the test media under flow through conditions. Exposure concentrations were analytically verified at days 7, 14, 21, and 28. The analytical values show that the nominal concentrations of 0.20 and 0.02 mg/L were maintained during the study (0.2mg/L nominal: 0.194 - 0.199 mg/L; 0.02 mg/L nominal: 0.0194-0.0202 mg/L).At days 7, 14, 21, 25 and 28 two fish each were removed from each test group. Body weight was determined. The fish were homogenised and extracted with acetonitrile and n-hexane. The extract was analysed with HPLC.Due to the low bioaccumulation potential, no depuration phase was required.
Nominal and measured concentrations:
nominal concentration 0.5 mg/L:
analytically measured concentrations:
day 4: 0.199 mg/L
day 7: 0.196 mg/L;
day 14: 0.194 mg/L
day 21: 0.198 mg/L
day 28: 0.196 mg/L

nominal concentration 0.05 mg/L
analytically measured concentrations:
day 4: 0.0200 mg/L
day 7: 0.0202 mg/L
day 14: 0.0194 mg/L
day 21: 0.0201 mg/L
day 28: 0.0195 mg/L
Details on estimation of bioconcentration:
according to guideline
Key result
Conc. / dose:
0.2 mg/L
Type:
BCF
Value:
40 dimensionless
Basis:
normalised lipid fraction
Time of plateau:
7 d
Calculation basis:
steady state
Key result
Conc. / dose:
0.02 mg/L
pH:
7
Type:
BCF
Value:
38 dimensionless
Basis:
normalised lipid fraction
Time of plateau:
7 d
Calculation basis:
steady state
Details on kinetic parameters:
steady state was reached within 7 days. Due to the low uptake, no depuration phase was necessary
Metabolites:
none
Details on results:
- Mortality of test organisms: no effects
- Behavioural abnormalities:no effects
- Observations on body length and weight:no effects
- Other biological observations: no effects
- Bound residues forming a plateau: no
- Mortality and/or behavioural abnormalities of control: no
- Loss of test substance during test period: no
- Results with vehicle control: no effects
Validity criteria fulfilled:
yes
Conclusions:
The test material showed no relevant bioaccumulation at concentrations at or above the water solubility. The steady state BCF values were at 39. The test material showed no relevant bioaccumulation (BCF < 2000).

Description of key information

Bioaccumulation was tested in fish  (Cyprinus carpio) exposed for 28 days to the test material at concentrations of 0.5 and 0.05 mg/L in a flow through system. Regular concentration control analysis of the test media showed that the test material concentrations were maintained throughout the exposure period and that the test material was present in dissolved form, thus proving steady state exposure conditions. No signs of toxicity were observed throughout the study. Steady state BCFs of 39 were determined. Due to the low bioaccumulation potential, no depuration phase was required.

The steady state BCF values were at 39 thus the test material showed no relevant bioaccumulation (BCF < 2000).

Key value for chemical safety assessment

BCF (aquatic species):
39 dimensionless

Additional information