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Diss Factsheets
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EC number: 223-773-8 | CAS number: 4066-02-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1991-10-14 - 1991-10-17
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- test procedure in accordance with national standard methods with acceptable restrictions
- Remarks:
- The study was conducted under GLP according to DIN 38 412 part 9 on the registered substance itself. The method is to be considered scientifically reasonable with minor deficiencies in documentation, but the available information allows the conclusion that the test was properly conducted. Also, the test item showed precipitation in the test vessels.
- Qualifier:
- according to guideline
- Guideline:
- other: DIN 38 412 Teil 9, Bestimmung der Hemmwirkung von Wasserinhaltsstoffen auf Grünalgen (Scenendesmus-Zellvermehrungs-Hemmtest) (L9), May 1989
- Deviations:
- no
- GLP compliance:
- yes
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Weighing-in directly
- Controls: inoculum, in culture medium, and dilution water
- Evidence of undissolved material (e.g. precipitate, surface film, etc): precipitate as sediment and particles on surface - Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- TEST ORGANISM
- Common name: green algae
- Strain: Scenedesmus subspicatus CHODAT
- Source (laboratory, culture collection): Pflanzenphysiologisches Institut der Universität, 3400 Göttingen, Germany
- Method of cultivation: In laboratory for water biology (WV-Umweltschutz) in light cabinet (PVP) at 23 ± 2 °C and a photosynthetically effective radiant flux density of 120 Micro-Einstein / m² * s; weekly inoculation of stem cultures
ACCLIMATION
- Any deformed or abnormal cells observed: none noted - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
- Post exposure observation period:
- none
- pH:
- 8.5 - 11.1
- Nominal and measured concentrations:
- 0, 320, 1000 mg/L (nominal)
- Details on test conditions:
- TEST SYSTEM
- Test vessel: Erlenmeyer flask
- Material, size, headspace, fill volume: 300 ml total volume, 100 ml fill volume
- Initial cells density: 10,000 cells/ml
- Control end cells density: 771.000 cells/ml
GROWTH MEDIUM
- Standard medium used: according to chapter 12.1 of DIN 38 412 part 9
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: deionized water, additionally treated with Millipore system
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: Microcellcounter Sysmex F-300 (Digitana), daily
- Chlorophyll measurement: no
TEST CONCENTRATIONS
- Test concentrations: 0, 320, 1000 mg/ml - Reference substance (positive control):
- no
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Details on results:
- - Exponential growth in the control (for algal test): yes
- Observation of abnormalities (for algal test): none noted
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: In all testing solutions unsolved fractions of the test item remained as sediment resp. swam on the surface of the test medium.
- Effect concentrations exceeding solubility of substance in test medium: 320, 1000 mg/L - Validity criteria fulfilled:
- not specified
- Conclusions:
- The study was conducted under GLP according to DIN 38 412 part 9 on the registered substance itself. The method is to be considered scientifically reasonable with minor deficiencies in documentation, but the available information allows the conclusion that the test was in general properly conducted. Hence, the results can be considered as reliable to assess the effects of Phenol, 2,2'-methylenebis-(6-cyclohexyl-4-methyl) to aquatic organisms, here, green algae.
Taking into account the determined effect values after 72h with regard to growth rate and biomass, based on nominal concentrations, i.e. EC10 ≥ 1000 mg/L, and EC50 > 1000 mg/L, the test item does not need to be classified as hazardous to the aquatic environment according to Regulation (EC) No. 1272/2008, neither acute nor long-term.
As noted in the test report, the test item was not completely solved in any of the test solutions. This implies that the actual concentrations are below the nominal ones. Since no concrete numbers are available, a classification as aquatic chronic should be considered. Water solubility was determined as 0.034 mg/L, triggering hence classification as aquatic chronic cat. 4. - Executive summary:
In an 72 hour acute toxicity study, the cultures of Scenedesmus subspicatus CHODAT were exposed to Phenol, 2,2'-methylenebis-(6-cyclohexyl-4-methyl) at nominal concentrations of 0, 320, and 1000 mg/L under static conditions in accordance with the DIN 38 412 part 9. The EC10and EC50 values based on growth rate and biomass were >1000 mg/L (nominal).
There were no compound related phytotoxic effects noted.
This toxicity study is classified as acceptable.
Reference
Description of key information
Toxicity to algae: EC10 > 1000 mg/L, EC50 > 1000 mg/L (nominal) for Scenedesmus subspicatus CHODAT (DIN 38 412 part 9, static)
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 1 000 mg/L
- EC10 or NOEC for freshwater algae:
- 1 000 mg/L
Additional information
The chosen endpoint is the only available study. It was conducted under GLP according to DIN 38 412 part 9 on the registered substance itself. The method is to be considered scientifically reasonable with minor deficiencies in documentation, but the available information allows the conclusion that the test was in general properly conducted. Hence, the results can be considered as reliable to assess the effects of Phenol, 2,2'-methylenebis-(6-cyclohexyl-4-methyl) to aquatic organisms, here, green algae.
The results were, based on nominal concentrations, EC10 >1000 mg/L, and EC50 > 1000 mg/L, which do not trigger classification as hazardous to the aquatic environment according to Regulation (EC) No. 1272/2008, neither acute nor long-term.
Taking into account the determined water solubility of 0.034 mg/L, the safety net classification as acquatic chronic 4 is triggered.
There is no indication that the study and hence the result and conclusions are not reliable, and so no additional data is required, no data gaps were identified.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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