Pentan-1-ol

Repeated dose toxicity: inhalation

Administrative data

Endpoint:

sub-chronic toxicity: inhalation

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference

Reference 1

Reference Type:

publication

Title:

Blood and Brain n-Pentanol in Inhalation Exposure

Author:

Savolainen S, Pfaeffli and Elovaara

Year:

1985

Bibliographic source:

Acta pharmacol et toxicol 56: 260-264

Reference 2

Reference Type:

secondary source

Title:

No information

Author:

RIFM

Year:

2009

Bibliographic source:

RIFM database

Materials and methods

Principles of method if other than guideline:

Analysis of tissue distribution of the test substance in rats after inhalation exposure (with body weight changes evaluation)

GLP compliance:

not specified

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

Pentan-1-ol

EC Number:

200-752-1

EC Name:

Pentan-1-ol

Cas Number:

71-41-0

Molecular formula:

C5H12O

IUPAC Name:

pentan-1-ol

Details on test material:

- Name of test material (as cited in study report): n-pentanol
- Analytical purity: analytical grade

Test animals

Species:

rat

Strain:

Wistar

Sex:

male

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Age at study initiation: 3 months
- Weight at study initiation: 254 ± 29 g for test group, 253 ± 22 g in control group

ENVIRONMENTAL CONDITIONS
- Photoperiod (hrs dark / hrs light): 12 / 12

Administration / exposure

Route of administration:

inhalation: vapour

Type of inhalation exposure:

not specified

Vehicle:

other: unchanged (no vehicle)

Remarks on MMAD:

MMAD / GSD: not given

Details on inhalation exposure:

GENERATION OF TEST ATMOSPHERE / CHAMBER DESCRIPTION
- Exposure apparatus: dynamic chamber (1 m3) technique with continous I.R. control

Analytical verification of doses or concentrations:

not specified

Duration of treatment / exposure:

7 - 14 weeks

Frequency of treatment:

5 days per week, 6 hours daily

Doses / concentrations

Doses / concentrations 1

Remarks:

Doses / Concentrations:
100, 300, 600 ppm
Basis:
nominal conc.

Doses / concentrations 2

Remarks:

Doses / Concentrations:
0.36, 1.08, 2.16 mg/l
Basis:
nominal conc.

No. of animals per sex per dose:

10

Control animals:

yes, sham-exposed

Details on study design:

Post-exposure period: none

Examinations

Observations and examinations performed and frequency:

CAGE SIDE OBSERVATIONS: No data

DETAILED CLINICAL OBSERVATIONS: No data

BODY WEIGHT: Yes

FOOD CONSUMPTION: no data

FOOD EFFICIENCY: no data

WATER CONSUMPTION: No data

OPHTHALMOSCOPIC EXAMINATION: No data

HAEMATOLOGY: No data

CLINICAL CHEMISTRY: No data

URINALYSIS: No data

NEUROBEHAVIOURAL EXAMINATION: No data

Sacrifice and pathology:

The tissue distribution of test substance was analysed at necropsy

Other examinations:

- alcohol and aldehyde concentrations in the brain were determined by gas chromatography
- calcium aggregated regated microsomes from liver and kidneys were analyzed for their cytochrome P-450 content
- Cytosolic n-pentanol dehydrogenase activities were also assayed in liver and kidney

Statistics:

The least squares method was used to find out the mathematical relationship of the inhaled and blood n-pentanol concentrations. All results were statistically evaluated with variance analysis.

Results and discussion

Results of examinations

Details on results:

BODY WEIGHT AND WEIGHT GAIN
The weight of the exposed animals did not differ from that of controls at the end of the experiment:
100 ppm: 435 ± 4 g
300 ppm: 397 ± 74 g
600 ppm: 400 ± 34 g
control: 419 ± 29 g

Effect levels

Dose descriptor:

NOAEL

Effect level:

2.16 mg/L air (nominal)

Based on:

test mat.

Sex:

male

Basis for effect level:

other: only body weight data were considered (no changes observed); no additional data provided

Target system / organ toxicity

Critical effects observed:

not specified