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Diss Factsheets
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EC number: 249-320-4 | CAS number: 28940-11-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to microorganisms, other
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Study period:
- August 11 to September 12, 2005
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Remarks:
- This study was performed according to OECD Guideline 301F with GLP statement. All validity criteria were fulfilled.
- Reason / purpose for cross-reference:
- reference to same study
- Qualifier:
- according to guideline
- Guideline:
- other: OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- Swiss GLP compliance programme (inspected on March 31-April 01, 2005 / signed on June 2005)
- Specific details on test material used for the study:
- - Physical state: White crystals
- Analytical monitoring:
- no
- Vehicle:
- no
- Test organisms (species):
- activated sludge, domestic
- Details on inoculum:
- Fresh activated sludge from a biological waste water treatment plant treating predominantly domestic sewage (City of Geneva, Peney-Dessous) was used.
The sludge is collected in the morning, washed three times in the mineral medium (by centrifuging at 1000 g for 10 minutes, discarding the supernatant and resuspending in mineral medium) and kept
aerobic until being used on the same day. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 32 d
- Remarks on exposure duration:
- Exposure duration of the biodegradation study
- Hardness:
- No data
- Test temperature:
- 22°C
- pH:
- The pH is measured and if necessary adjusted to 7.4 ± 0.2 with phosphoric acid or potassium hydroxide
- Dissolved oxygen:
- No data
- Salinity:
- Not applicable
- Conductivity:
- No data
- Nominal and measured concentrations:
- 100 mg/L
- Details on test conditions:
- The toxicity of the test substance for the inoculum was checked in the biodegradation study. A pair of flasks of the volumetric respirometer was filled with mineral medium + test substance (100 mg/I) + reference substance (Benzoic acid, sodium salt 100 mg/l) + inoculum and their respirations were recorded according to the OECD Guideline 301F. If they are lower than those of the flasks containing mineral medium + reference substance ( 100 mg/l) + inoculum, the test substance can be assumed to be inhibitory to the inoculum used.
- Reference substance (positive control):
- yes
- Remarks:
- benzoic acid, sodium salt 100 mg/L
- Key result
- Duration:
- 32 d
- Dose descriptor:
- NOEC
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: O2 consumption
- Details on results:
- The curves obtained with the reference substance alone and with the test substance + reference substance showed no toxic effect of the test substance to the micro-organisms at the test concentration (100 mg/L).
- Results with reference substance (positive control):
- See table in "Any other information on results incl. tables".
- Reported statistics and error estimates:
- None
- Validity criteria fulfilled:
- not applicable
- Conclusions:
- Under the test conditions, test substance should be regarded as not toxic to microorganisms up to 100 mg/L.
- Executive summary:
The toxicity effect of the test substance to microorganisms has been determined in the Manometric Respirometry Test according to the OECD Guideline No. 301F, EU Method C.4 -D with GLP compliance, in a toxicity control. A nominal concentration of test substance (100 mg/L) was introduced in the system. The results obtained with the reference substance alone (benzoic acid, sodium salt) and with the test substance + reference substance show no toxic effect of the test substance to the micro-organisms at the test concentration (100 mg/L).
In conclusion, test substance should be regarded as not toxic to microorganisms up to 100 mg/L, under the test conditions.
Reference
Table 6.1.7/1: Biodegradability of the reference substance and test substance toxicity control
|
Days |
5 |
7 |
14 |
21 |
28 |
32 |
BOD sludge |
1st flask 2nd flask Mean |
7.0 4.0 5.5 |
11.0 8.0 9.5 |
17.0 17.0 17.0 |
25.0 26.0 25.5 |
28.0 29.0 28.5 |
28.0 29.0 28.5 |
BOD reference substance |
1st flask 2nd flask Mean |
127.0 135.0 131.0 |
143.0 150.0 146.5 |
161.0 168.0 164.5 |
173.0 179.0 176.0 |
181.0 187.0 184.0 |
181.0 187.0 184.0 |
% biodegradation reference substance |
1st flask 2nd flask Mean |
73 78 75 |
80 84 82 |
86 91 89 |
89 92 90 |
92 95 93 |
92 95 93 |
BOD refer. + test substance (toxicity control) |
1st flask 2nd flask Mean |
148.0 162.7 155.3 |
157.4 172.7 165.0 |
168.7 186.8 177.8 |
176.0 197.8 186.9 |
182.1 205.8 193.9 |
183.1 206.8 194.9 |
BOD: Biological Oxygen Demand, mg O2/L, adjusted to nominal concentrations.
As BOD values of the toxicity control are not lower than those of the flasks containing mineral medium + reference substance ( 100 mg/l) + inoculum (BOD reference substance), the test substance can be assumed to be not toxic to the inoculum used.
Description of key information
OECD Guideline 301F, EU Method C.4-D, GLP, Weight of evidence, validity 1:
Not toxic to microorganisms up to 100 mg/L
Key value for chemical safety assessment
Additional information
No activated sludge respiration inhibition test is available on the registered substance. However, a toxicity control was performed in the biodegradation study (Givaudan, 2005) performed on the registered substance and can be used to fulfil this endpoint.
The toxicity effect of the test substance to microorganisms has been determined in the Manometric Respirometry Test according to the OECD Guideline No. 301F, EU Method C.4 -D with GLP compliance, in a toxicity control. A nominal concentration of test substance (100 mg/L) was introduced in the system. The results obtained with the reference substance alone (benzoic acid, sodium salt) and with the test substance + reference substance shows no toxic effect of the test substance to the micro-organisms at the test concentration (100 mg/L).
In conclusion, test substance should be regarded as not toxic to microorganisms up to 100 mg/L, under the test conditions.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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