1-hydroxybenzotriazole

Toxicity to microorganisms

Link to relevant study record(s)

Reference

Endpoint:

toxicity to microorganisms

Type of information:

experimental study

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Experimental data from peer reviewed journal

Justification for type of information:

Experimental data from peer reviewed journal

Qualifier:

according to guideline

Guideline:

other: Refer below principle

Principles of method if other than guideline:

Microtox test: The test exposes luminescent organisms in Microtox Acute Reagent to aqueous samples, and measures the increase or decrease in light output by the test organisms.Reagent contains living luminescent bacteria that have been grown under optimal conditions, harvested,and then lyophilized (freeze-dried). The lyophilized bacteria are rehydrated with Reconstitution Solution to provide a ready-to-use suspension of organisms. The test system measures the light output of the luminescent bacteria after they have been challenged by a sample and compares it to the light output of a control (reagent blank) that contains no sample. A difference in light output (between the sample and the control) is attributed to the effect of the sample on the organisms.

GLP compliance:

not specified

Analytical monitoring:

not specified

Vehicle:

not specified

Test organisms (species):

Photobacterium phosphoreum

Test type:

static

Water media type:

freshwater

Total exposure duration:

30 min

Remarks on exposure duration:

5-30 min. exposure period

Hardness:

No data

Test temperature:

15 degC

pH:

range of 5< pH <9

Dissolved oxygen:

No data

Salinity:

No data

Conductivity:

No data

Nominal and measured concentrations:

No data

Details on test conditions:

No data

Reference substance (positive control):

yes

Key result

Duration:

30 min

Dose descriptor:

EC50

Effect conc.:

86.4 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

other: viability and sensitivity

Validity criteria fulfilled:

not specified

Conclusions:

The median effective concentration (EC50) for the test substance, in microorganism (Photobacterium phosphoreum) in a 5-30 minute study on the basis of viability and sensitivity effect was observed to be 86.4 mg/L.

Executive summary:

The median effective concentration (EC50) for the test substance, in microorganism (Photobacterium phosphoreum) in a 5-30 minute study on the basis of viability and sensitivity effect was observed to be 86.4 mg/L.

Description of key information

Toxicity to microorganism:

Data available for the structurally similar read across chemicals has been reviewed to determine the effect of the test chemical.The studies are as mentioned below:

In first study ,inhibitory effects of test chemical on micro organism species Bacillus spp. was observed using different soil and water samples as inoculum. Test chemical analytically monitorized by thin-layer chromatography. A stock solution (0.276 mg/ml) was prepared in methanol,with an activity of 36 512 dpm/ul). Methanol were used as a vehicle in which test chemical dissolved. Test conducted under the static system for 192 hrs. During experiment the Effect of benzimidazole derivatives on maximum growth of Bacillus strains was observed to be at concentrations 500-1000 mg/l (reported as ug/ml) using tubes shaken in water bath at 30C; Klett-Summerson readings taken at intervals up to 192 h after inoculation. Based on the EC50 value, chemical consider to be nontoxic.

The median effective concentration (EC50) for the test substance, in microorganism (Photobacterium phosphoreum) in a 5-30 minute study on the basis of viability and sensitivity effect was observed to be 86.4 mg/L.

Key value for chemical safety assessment

EC50 for microorganisms:

86.4 mg/L

Additional information

Toxicity to microorganism:

Data available for the structurally similar read across chemicals has been reviewed to determine the effect of the test chemical.The studies are as mentioned below:

In first study ,inhibitory effects of test chemical on micro organism species Bacillus spp. was observed using different soil and water samples as inoculum. Test chemical analytically monitorized by thin-layer chromatography. A stock solution (0.276 mg/ml) was prepared in methanol,with an activity of 36 512 dpm/ul). Methanol were used as a vehicle in which test chemical dissolved. Test conducted under the static system for 192 hrs. During experiment the Effect of benzimidazole derivatives on maximum growth of Bacillus strains was observed to be at concentrations 500-1000 mg/l (reported as ug/ml) using tubes shaken in water bath at 30C; Klett-Summerson readings taken at intervals up to 192 h after inoculation. Based on the EC50 value, chemical consider to be nontoxic.

The median effective concentration (EC50) for the test substance, in microorganism (Photobacterium phosphoreum) in a 5-30 minute study on the basis of viability and sensitivity effect was observed to be 86.4 mg/L.