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EC number: 208-621-0 | CAS number: 535-87-5
Endpoint summary
Administrative data
Description of key information
Biodegradation in water
Biodegradability of 3,5-Diaminobenzoic acid is predicted using QSAR toolbox version 3.4 (2017). Test substance undergoes 33.25% degradation by CO2 evolution parameter in 28 days. Thus, based on percentage degradation, the test chemical 3,5-Diaminobenzoic acid was estimated to be not readily biodegradable in water.
Biodegradation in water and sediment
Estimation Programs Interface (EPI Suite, 2017) prediction model was run to predict the half-life in water and sediment for the test compound 3,5-Diaminobenzoic acid (CAS No. 535 -87 -5). If released in to the environment, 35% of the chemical will partition into water according to the Mackay fugacity model level III and the half-life period of 3,5-Diaminobenzoic acid in water is estimated to be 37.5 days (900 hrs). The half-life (37.5 days estimated by EPI suite) indicates that the chemical is not persistent in water and the exposure risk to aquatic animals is moderate to low whereas the half-life period of 3,5-Diaminobenzoic acid in sediment is estimated to be 337.5 days (8100 hrs). However, as the percentage release of test chemical into the sediment is less than 1% (i.e, reported as 0.0835%), indicates that 3,5-Diaminobenzoic acid is not persistent in sediment.
Biodegradation in soil
The half-life period of 3,5-Diaminobenzoic acid (CAS No. 535 -87 -5) in soil was estimated using Level III Fugacity Model by EPI Suite version 4.1 estimation database (EPI suite, 2017). If released into the environment, 64.9% of the chemical will partition into soil according to the Mackay fugacity model level III. The half-life period of 3,5-Diaminobenzoic acid in soil is estimated to be 75 days (1800 hrs). Based on this half-life value of 3,5-Diaminobenzoic acid, it is concluded that the chemical is not persistent in the soil environment and the exposure risk to soil dwelling animals is moderate to low.
Additional information
Biodegradation in water
Various predicted data for the target compound 3,5-Diaminobenzoic acid (CAS No. 535-87-5) and the study for its read across substance were reviewed for the biodegradation end point which are summarized as below:
Biodegradability of 3,5-Diaminobenzoic acid (CAS no. 535-87-5) is predicted using QSAR toolbox version 3.4 (2017). Test substance undergoes 33.25% degradation by CO2 evolution parameter in 28 days. Thus, based on percentage degradation, the test chemical 3,5-Diaminobenzoic acid was estimated to be not readily biodegradable in water.
Estimation Programs Interface Suite (EPI suite, 2017) was run to predict the biodegradation potential of the test compound 3,5-Diaminobenzoic acid (CAS no. 535 -87 -5) in the presence of mixed populations of environmental microorganisms. The biodegradability of the substance was calculated using seven different models such as Linear Model, Non-Linear Model, Ultimate Biodegradation Timeframe, Primary Biodegradation Timeframe, MITI Linear Model, MITI Non-Linear Model and Anaerobic Model (called as Biowin 1-7, respectively) of the BIOWIN v4.10 software. The results indicate that 3,5-Diaminobenzoic acid is expected to be not readily biodegradable.
In a weight of evidence study of read across substance m-phenylenediamine (CAS no. 108 -45 -2) which is having a percentage similarity of 50-60% with the target chemical 3,5-Diaminobenzoic acid (J-CHECK, 2016), biodegradation experiment was conducted for 28 days for evaluating the percentage biodegradability of read across substance m-phenylenediamine (CAS no. 108 -45 -2). Concentration of inoculum i.e, sludge used was 30 mg/l and initial test substance conc. used in the study was 100 mg/l, respectively. The percentage degradation of read across substance was determined 2, 0 and 0% by BOD, TOC removal and HPLC parameter in 28 days. Thus, based on percentage degradation, m-phenylenediamine is considered to be not readily biodegradable in nature.
Another weight of evidence study (HSDB, 2016) of biodegradation was conducted for 30 days for evaluating the percentage biodegradability of read across substance 3,5-Dichloroaniline (CAS no. 626 -43 -7) which is having a percentage similarity of 50-60% with the target chemical 3,5-Diaminobenzoic acid. The study was performed according to OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test). Activated sludge was used as a test inoculum. The percentage degradation of read across substance was determined to be 0% by BOD parameter in 30 days. Thus, based on percentage degradation, 3,5 -Dichloroaniline is considered to be not readily biodegradable in nature.
In an additional weight of evidence study (J-CHECK, 2016), biodegradation was conducted for 28 days for evaluating the percentage biodegradability of read across substance 4-Aminoazobenzene (CAS no. 60 -09 -3). Concentration of inoculum i.e, sludge used was 30 mg/l and initial test substance conc. used in the study was 100 mg/l, respectively. The percentage degradation of read across substance was determined 0% by BOD and HPLC parameter in 28 days. Thus, based on percentage degradation, 4 -Aminoazobenzene is considered to be not readily biodegradable in nature.
On the basis of above results for target and read across substance, it can be concluded that the test substance3,5-Diaminobenzoic acidcan be expected to be not readily biodegradable in nature.
Biodegradation in water and sediment
Estimation Programs Interface (EPI Suite, 2017) prediction model was run to predict the half-life in water and sediment for the test compound 3,5-Diaminobenzoic acid (CAS No. 535 -87 -5). If released in to the environment, 35% of the chemical will partition into water according to the Mackay fugacity model level III and the half-life period of 3,5-Diaminobenzoic acid in water is estimated to be 37.5 days (900 hrs). The half-life (37.5 days estimated by EPI suite) indicates that the chemical is not persistent in water and the exposure risk to aquatic animals is moderate to low whereas the half-life period of 3,5-Diaminobenzoic acid in sediment is estimated to be 337.5 days (8100 hrs). However, as the percentage release of test chemical into the sediment is less than 1% (i.e, reported as 0.0835%), indicates that 3,5-Diaminobenzoic acid is not persistent in sediment.
Biodegradation in soil
The half-life period of 3,5-Diaminobenzoic acid (CAS No. 535 -87 -5) in soil was estimated using Level III Fugacity Model by EPI Suite version 4.1 estimation database (EPI suite, 2017). If released into the environment, 64.9% of the chemical will partition into soil according to the Mackay fugacity model level III. The half-life period of 3,5-Diaminobenzoic acid in soil is estimated to be 75 days (1800 hrs). Based on this half-life value of 3,5-Diaminobenzoic acid, it is concluded that the chemical is not persistent in the soil environment and the exposure risk to soil dwelling animals is moderate to low.
On the basis of available information, the test substance 3,5 -Diaminobenzoic acid can be considered to be not readily biodegradable in nature.
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