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Diss Factsheets
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EC number: 204-934-1 | CAS number: 129-17-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: oral
Administrative data
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Data is from peer reviewed journal
Data source
Reference
- Reference Type:
- publication
- Title:
- Acute (Mouse and Rat) and Short-term (Rat) Toxicity Studies on Blue VRS
- Author:
- D. E. Hall and I. F. Gaunt
- Year:
- 1 967
- Bibliographic source:
- Fd Cosmet. Toxicol Vol. 5, pp. 165-170
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: as below
- Principles of method if other than guideline:
- The acute toxic effect of Blue VRS was evaluated in rats by a single oral dosage
- GLP compliance:
- not specified
- Test type:
- other: No data
- Limit test:
- yes
Test material
- Reference substance name:
- Hydrogen [4-[4-(diethylamino)-2',4'-disulphonatobenzhydrylidene]cyclohexa-2,5-dien-1-ylidene]diethylammonium, sodium salt
- EC Number:
- 204-934-1
- EC Name:
- Hydrogen [4-[4-(diethylamino)-2',4'-disulphonatobenzhydrylidene]cyclohexa-2,5-dien-1-ylidene]diethylammonium, sodium salt
- Cas Number:
- 129-17-9
- Molecular formula:
- C27H32N2O6S2.Na
- IUPAC Name:
- hydrogen [4-[4-(diethylamino)-2',4'-disulphonatobenzhydrylidene]cyclohexa-2,5-dien-1-ylidene]diethylammonium, sodium salt
- Details on test material:
- - Name of test material (as cited in study report):Blue VRS- Molecular formula (if other than submission substance):C27H31N2O6S2.Na- Molecular weight (if other than submission substance):566.672g/mole- Substance type:Organic- Physical state:SolidPurity94.5%- Impurities (identity and concentrations):0.2% subsidiary dyes
Constituent 1
Test animals
- Species:
- rat
- Strain:
- other: SPF Carworth Farm E
- Sex:
- male/female
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- not specified
- Details on oral exposure:
- No data available
- Doses:
- 10000 mg/Kg bw
- No. of animals per sex per dose:
- 5 male, 5 female
- Control animals:
- not specified
- Details on study design:
- Details on study design- Duration of observation period following administration: 7 days- Frequency of observations and weighing: No data available- Necropsy of survivors performed: No data available- Other examinations performed: Clinical sign were observed
- Statistics:
- The LDs0 values with 95% confidence limits calculated according to Litchfield & Wilcoxon (1949).
Results and discussion
- Preliminary study:
- No data avaiable
Effect levels
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- > 10 000 mg/kg bw
- Based on:
- test mat.
- Remarks on result:
- other: No effect on survival and clinical sign
- Mortality:
- No effect on survival of treated male and female rats were observed.
- Clinical signs:
- other: No effect on clinical sign of treated male and female rats were observed.
- Gross pathology:
- No data avaiable
- Other findings:
- Distinct blue coloration of the skin showed that some of the colouring had been absorbed although substantial amounts were excreted in the faeces.
Applicant's summary and conclusion
- Interpretation of results:
- practically nontoxic
- Remarks:
- Migrated informationCriteria used for interpretation of results: EU
- Conclusions:
- LD50 was considered to be > 5000 mg/kg bw when in SPF Carworth Farm E male and female rats were treated with Blue VRS orally by gavage.
- Executive summary:
- In a acute oral toxicity study, SPF Carworth Farm E male and female rats were treated with Blue VRS orally by gavage. No effect on survival and clinical sign were observed in treated rats. Distinct blue coloration of the skin showed that some of the colouring had been absorbed although substantial amounts were excreted in the faeces. Therefore, LD50 was considered to be > 5000 mg/kg bw when SPF Carworth Farm E male and female rats were treated with Blue VRS orally by gavage.
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