Disodium 2,5-dichloro-4-(5-hydroxy-3-methyl-4-(sulphophenylazo)pyrazol-1-yl)benzenesulphonate

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

key study

Study period:

From July 31st to August 02nd, 2001

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

test procedure in accordance with generally accepted scientific standards and described in sufficient detail

Justification for type of information:

Justification for Read Across is reported in the endpoint summary and it is further detailed in the report attached to the IUCLID section 13.

Reason / purpose for cross-reference:

read-across source

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Version / remarks:

part 1

GLP compliance:

not specified

Details on test solutions:

A solution in dilution water was prepared at the nominal concentration of 125 mg/l. pH of the solution was 8.2.

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Daphnia magna, Stratus.
- Age at study initiation: young animals aged 0-2 4 hours were used.
- Food type: Tetra Animin®.
- Frequency of feeding: twice a week.

ACCLIMATION
- Acclimation conditions: daphnids were maintained at 20 ± 1°C and under a photoperiod of 16/8 hours light/dark. 5 organisms per litre.

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 d

Test temperature:

20 ± 1 °C

pH:

Control: 7.9 - 8.6
Test vessels: 8.2 - 8.3

Dissolved oxygen:

Control: 7.8 - 8.8 mg/l
Test vessels: 7.8 - 8.4

Nominal and measured concentrations:

0 and 125 mg/l (nominal)

Details on test conditions:

TEST SYSTEM
- Test vessel: 50 ml beaker according to DIN, HF.
- No. of organisms per vessel: 5.
- No. of vessels per concentration: 4 replicates.
- No. of vessels per control: 4 replicates.
- Biomass loading rate: use of 5 animals each in 20 ml of sample.

TEST MEDIUM
- Preparation of dilution water: M4 Medium.
- Composition of M4 Medium: CaCl2 x 2H2O 177.9 mg/l, MgSO4 x 7H2O 67.4 mg/l, NaCl 17.8 mg/l, Na2SiO3 x 9H20 1000 µg/l, NaNO3 27.4 µg/l, KH2PO4 14.3 µg/l, K2HPO4 18.4 µg/l, Na2EDTA x 2H2O 2500 µg/l, FeSO4 x 7H2O 995.5 µg/l, H3BO3 2859.5 µg/l, MnCl2 x 4H2O 380.5 µg/l, LiCl 310.5 µg/l, RbCl 71.0 µg/l, SrCl2 x 6H2O 152.0 µg/l, NaBr 16.0 µg/l, Na2MoO4 x 2H2O 63.0 µg/l, CuCl2 x 6H2O 16.5 µg/l, ZnCl2 13.0 µg/l, CoCl2 x 6H2O 10.0 µg/l, KI 3.25 µg/l, Na2SeO3 2.19 µg/l, NH4VO3 0.575 µg/l, Thiamin x HCl 75.0 ng/l, Cyanocobalamin 1.0 ng/l, Biotin 0.75 ng/l
- Composition of dilution water: CaCl2 x 2H2O 177.9 mg/l, MgSO4 x 7H2O 67.4 mg/l, NaCl 17.8 mg/l.
- Composition of tap water: Ca+ 31.6 mg/l, Mg2+ 5.5 mg/l, K+ 3.2 mg/l, Na+ 11.2 mg/l.

EFFECT PARAMETERS MEASURED
Immobilization after 48 hours.

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 125 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Test results

Replicate	Conc. (mg/l)	N. of daphnids	Inmmobilization after 24 hrs		Inmmobilization after 48 hrs
			Absolute	Percentage	Absolute	Percentage
Blank 1	0	5	0	5	0	5
Blank 2	0	5	1		1
Blank 3	0	5	0		0
Blank 4	0	5	0		0
Test item 1	125	5	0	0	0	0
Test item 2	125	5	0		0
Test item 3	125	5	0		0
Test item 4	125	5	0		0

Summary of the test conditions

Conc. (mg/l)	pH		O2 (mg/l)
	0 hrs	48 hrs	0 hrs	48 hrs
0	7.9	8.6	8.8	7.8
125	8.2	8.3	8.4	7.8

Validity criteria fulfilled:

yes

Remarks:

ion the control not more than 10 % of the Daphnia have been immobilized and the dissolved oxygen concentration at the end of the test was higher than 60 %

Conclusions:

EC50 (48h) > 125 mg/l (nominal)

Executive summary:

Method

The short term toxicity to aquatic invertebrates was determined using the OECD Guideline 202, part I (Daphnia sp. Acute Immobilisation Test). A solution in dilution water was prepared at the nominal concentration of 125 mg/l. pH of the solution was 8.2.

Results

EC50 (48h) > 125 mg/l (nominal)

Description of key information

EC50 (48h) > 125 mg/l (nominal)

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

125 mg/L