3-phenylpropionyl chloride

Genetic toxicity: in vitro

Administrative data

Endpoint:

in vitro gene mutation study in bacteria

Type of information:

experimental study

Adequacy of study:

weight of evidence

Reliability:

4 (not assignable)

Rationale for reliability incl. deficiencies:

secondary literature

Justification for type of information:

Data from secondary literature

Data source

Reference

Reference Type:

secondary source

Title:

Mutagenicity of Chlorinated Aromatic Hydrocarbons containing Oxygen

Author:

Tadamichi Ohkubo, Sumio Gotoz, Osamu Endoz, Tetsuhito Hayashi, Etsuo Watanabe and Hideaki Endo

Year:

1996

Bibliographic source:

JEC 6 (4), 533-540, 1996

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

other: Test guideline 9 of the Department of Labor, Japan

Principles of method if other than guideline:

Mutagenicity Tests of 3 phenylpropionylchloride was performed by using Bacterial Gene mutation assay

GLP compliance:

not specified

Type of assay:

bacterial reverse mutation assay

Test material

Test material information

Constituent 1

Reference substance name:

3-phenylpropionyl chloride

EC Number:

211-443-6

EC Name:

3-phenylpropionyl chloride

Cas Number:

645-45-4

Molecular formula:

C9H9ClO

IUPAC Name:

3-phenylpropanoyl chloride

Test material form:

solid

Details on test material:

- Name of test material (as cited in study report): 3 phenylpropionylchloride- Molecular formula:C9H9ClO- Molecular weight :168.622- Substance type: organic- Physical state: solid -Purity: No data available - Impurities (identity and concentrations): No data avaliable

Specific details on test material used for the study:

- Name of test material (as cited in study report): 3 phenylpropionylchloride- Molecular formula:C9H9ClO- Molecular weight :168.622- Substance type: organic- Physical state: solid -Purity: No data available - Impurities (identity and concentrations): No data avaliable

Method

Target gene:

Histidine and Tryptophan

Species / strain

Species / strain 1

Species / strain / cell type:

S. typhimurium, other: TA98, TA100,TA104

Details on mammalian cell type (if applicable):

no data available

Additional strain / cell type characteristics:

not specified

Species / strain 2

Species / strain / cell type:

E. coli WP2 uvr A pKM 101

Details on mammalian cell type (if applicable):

No data available

Additional strain / cell type characteristics:

not specified

Cytokinesis block (if used):

no data available

Metabolic activation:

with and without

Metabolic activation system:

Enzyme with polychlorinated biphenyl (PCB, Kanechlor-500) and (P-450) induced the Sprague-Dawley male rats S9 mix

Test concentrations with justification for top dose:

0-1000 µg/plate

Vehicle / solvent:

- Vehicle(s)/solvent(s) used: DMSO - Justification for choice of solvent/vehicle: Test substance soluble in DMSO

Controls

Untreated negative controls:

not specified

Negative solvent / vehicle controls:

yes

Remarks:

DMSO

True negative controls:

not specified

Positive controls:

not specified

Details on test system and experimental conditions:

Method of application: Pre incubation DURATIONPreincubation period: at 37°C in a water bath, 20 minutes, 80 times/minExposure duration: at 37°C,48-60 hoursExpression time (cells in growth medium):Selection time (if incubation with a selection agent):No dataFixation time (start of exposure up to fixation or harvest of cells):No dataNUMBER OF REPLICATIONS: No dataDETERMINATION OF CYTOTOXICITY- Method: mitotic index; cloning efficiency; relative total growth;- other: No data

Rationale for test conditions:

No data available

Evaluation criteria:

There is a dose-response relationship between the (dose) and the response (the return rate variability colonies). If the response had more than twice the control (natural variation number of colonies ) the mutagenicity is positive (positive); dose-response relationship if accepted ,but false positive if the response is two times less than 1.5 times greater than the control (pseudopositive),Further , if or when the response dose-response relationship was not observed is less than 1.5 times the control were negative (negative).

Statistics:

No data available

Results and discussion

Test results

Test results 1

Species / strain:

S. typhimurium, other: TA98, TA100,TA104

Metabolic activation:

with and without

Genotoxicity:

positive

Cytotoxicity / choice of top concentrations:

not specified

Vehicle controls validity:

valid

Untreated negative controls validity:

not specified

Positive controls validity:

not specified

Test results 2

Species / strain:

E. coli WP2 uvr A pKM 101

Metabolic activation:

with

Genotoxicity:

positive

Cytotoxicity / choice of top concentrations:

not specified

Vehicle controls validity:

not specified

Untreated negative controls validity:

not specified

Positive controls validity:

not specified

Test results 3

Species / strain:

E. coli WP2 uvr A pKM 101

Metabolic activation:

without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

not specified

Vehicle controls validity:

not specified

Untreated negative controls validity:

not specified

Positive controls validity:

not specified

Additional information on results:

No data available

Remarks on result:

other: positive

Any other information on results incl. tables

Table 1:Mutagenicity of 3 phenylpropionylchloride by pre-incubation method employing S. typhimurium

Salmonella Typhimurium Strains	Revertants/µg without S9 mix	Revertants/µg with S9 mix
TA98	0.1	0.2
TA100	0.3	1.7
TA104	0.6	1.2

 

Table 2:Mutagenicity of 3 phenylpropionylchloride by pre-incubation method employing Escherichia ColiWP2 UVRA/pKM101

Compound	Revertants/µg without S9	Revertants/µg with S9
3 phenylpropionyl chloride	(0.5)	0.6

 

() = pseudopositive

Applicant's summary and conclusion

Conclusions:

Mutagenicity test of 3-phenylpropionyl chloride was carried out in Salmonella Typhimurium TA100,TA98, TA104 with and without metabolic activation (S9mix) produced mutation and when mutagenicity test was carried out in Escherichia Coli WP2 UVRA/pKM101 with and without metabolic activation (S9mix).3-phenylpropionyl chloride was mutagenic with metabolic activation and non-mutagenic without metabolic activation in Escherichia Coli WP2 UVRA/pKM101, therefore it is considered to be Positive with metabolic activation, negative without metabolic activation for gene mutation in vitro. Therefore it is considered to be positive for gene mutation in vitro

Executive summary:

Mutagenicity test of 3-phenylpropionyl chloride was done using pre-incubation method employing Salmonella typhimuriumTA100,TA 98,TA104 and Escherichia Coli WP2 UVRA/pKM101 with and without metabolic activation (S9mix). Enzyme with polychlorinated biphenyl (PCB, kanechlor-500) (p-450) induced Sprague-Dawley male rats (5 weeks old) of the S9 fraction cryopreservation product were prepared from the liver , test sample were diluted in dimethylsulfoxide(DMSO).Culture medium in large incubator (37⁰C ,48-60 hr ), and then the revertant colonies were counted in automatic colony counter.If the response had more than twice the control (natural variation number of colonies) the mutation is positive ; dose –response relationship if accepted , but false positive if the response is two times less than 1.5 times greater than the control (pseudopositive ). Further, if or when the response dose –response relationship was observed is less than 1.5 times the control were negative.

3- phenylpropionyl chloride was mutagenic in Salmonella typhimurium TA100,TA98,TA104 with and without metabolic activation and mutagenic with and non -mutagenic without metabolic activation in Escherichia Coli WP2UVRA/ pKM101 .Therefore it is considered to be positive with metabolic activation ,negative without metabolic activation for gene mutation in vitro.