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EC number: 616-020-3
CAS number: 7381-13-7
log Pow =1.775 ± 0.008
The study was performed using a HPLC with a
C18 column. Seven reference items with different retention times were
used to produce a calibration curve, since retention time on hydrophobie
columns and Pow are correlated .. First, a solution of the reference
items was analysed with HPLC, then a solution of the test item, and
finally again the solution with the reference items. Each analysis was
performed three times, totalling to nine runs. For each reference item,
the capacity factor K was calculated from the retention time of this
urea and the retention time of the respective reference item. A
calibration function (log K versus log Pow, linear fit) was determined
using the literature va lues for Pow of the reference items and the
retention times in the six determinations. The chromatogram of the test
item gave one peak (mean retention time = 4.894min). With the help of
the calibration function log K versus log Pow, the corresponding log Pow
was determined as 1.775 ± 0.008 this value being the mean of three
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