Oxacycloheptadec-10-en-2-one

Endpoint summary

Description of key information

Adsorption / desorption: The Adsorption Coefficient of test chemical was determined as per the HPLC method (OECD Guideline-121). The Log Koc value was determined to be 3.330±0.002 at 25°C.

 

Additional information

Adsorption / desorption: 

 

The adsorption coefficient Koc in soil and in sewage sludge of test chemical was determined by the Reverse Phase High Performance Liquid Chromatographic method according to OECD Guideline No. 121 for testing of Chemicals. The sample of test chemical was prepared by accurately weighing 10.8  mg and diluted with acetonitrile up to 10 mL to obtain the concentration of 400 mg/L. The pH of the resulting solution was found to be 6.4. The solutions of the test chemical and reference substances were prepared in acetonitrile each of the reference substances and test chemical were analysed by HPLC. The column was saturated with solvent (mobile phase) in isocratic mode on chromatographic conditions including the HPLC method, HPLC Model number Agilent Technologies,1260 Infinity II was used. Flow Rate was of 1.0ml/min.  Column used was ZORBAX Eclipse Plus C18, 4.6×100 mm, 3.5 µm. Guard columns used was C18. Mobile phase used was Acetonitrile: water with ratio of (55:45). Study was performed at a temperature of 25°C.  Diode Array Detector was used. Wavelength taken for study was 210 nm. Autosampler injector was used and the injector volume was 10 μl. Chem Station Open lab Control Panel was used as a data Acquisition software. After 20 min of column saturation with the mobile phase, sequential analysis of urea(for dead time: t₀), reference substances and test chemical was performed on HPLC. All the HPLC runs were performed in duplicates. Retention time t_R was measured to calculate the capacity factors k' it was then converted in log to obtain log k'. A standard curve between log k versus log K_oc was plotted to obtain the value of log K_oc of the test chemical. Reference substances 2-Nitrobenzamide, 3,5,Dinitrobenzamide, Phenol, Acetanilide, Naphthalene, and Phenanthrene were chosen. Individual solutions of these six reference substances were prepared in acetonitrile with concentrations of 100 mg/L. Formamide (at concentration of 1000 mg/L) was used as an unretained compound for the determination of the “dead time” t₀. Each of the reference substance and test substance were analysed by HPLC at 210 nm. The log K_oc value of all six reference substance was determined to be in the range of 0.9 to 3.4. The Adsorption Coefficient of test chemical was determined as per the HPLC method (OECD Guideline-121). The Log Koc value was determined to be 3.330±0.002 at 25°C. This log Koc value indicates that the test chemical has moderate sorption to soil and sediment and therefore have slow migration potential to ground water.