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EC number: 205-860-2 | CAS number: 156-60-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Basic toxicokinetics
Administrative data
- Endpoint:
- basic toxicokinetics in vitro / ex vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: This study was selected as the key study because the information provided for the hazard endpoint is sufficient for the purpose of classification and labelling and/or risk assessment.
Data source
Reference
- Reference Type:
- publication
- Title:
- Partition Coefficients of Low-Molecular-Weight Volatile Chemicals in Various Liquids and Tissues
- Author:
- Gargas ML, Burgess RJ, Voisard DE, Cason GH and Anderson ME
- Year:
- 1 989
- Bibliographic source:
- Tocicol. Appl. Pharmacol., 98:87-99
Materials and methods
- Objective of study:
- other: determining partition coefficients
- Principles of method if other than guideline:
- Determination of the tissue to air and liquid to air partition coefficients of the test substance in human and rat blood and rat tissues.
- GLP compliance:
- not specified
Test material
- Reference substance name:
- trans-dichloroethylene
- EC Number:
- 205-860-2
- EC Name:
- trans-dichloroethylene
- Cas Number:
- 156-60-5
- Molecular formula:
- C2H2Cl2
- IUPAC Name:
- (1E)-1,2-dichloroethene
- Details on test material:
- - Purity: > 98%
Constituent 1
- Radiolabelling:
- no
Test animals
- Species:
- rat
- Strain:
- other: CDF [F-344] CrlBr
- Sex:
- male
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Charles River Breeding Laboratory, Kinston, NY
- Weight at study initiation: between 200 and 300 g
- Housing: portable laminar air flow enclosure system
- Individual metabolism cages: no
- Diet: commercial food (Purina), ad libitum
- Water: tap water, ad libitum
ENVIRONMENTAL CONDITIONS
- Photoperiod (hrs dark / hrs light): 12/12
Administration / exposure
- Route of administration:
- other: tissues were removed from animals then combined with the test item
- Details on study design:
- The liquids and tissues utilized were: 0.9% NaCl (saline) solution, reagent-grade olive oil heparanised rat, and human blood, and rat liver, muscle and fat tissues. Tissues were prepared as homogenates in saline solution in a 1:3 tissue weight:saline volume ratio (assuming a tissue density of 1.0 g/mL). The saline:air partition coefficient determinations were necessary to account for the amount of partitioning due to saline in the tissue homogenates and the oil:air values were used for comparison with the fat & coefficients. The saline:air and oil:air partition coefficients were also used as independent variables for modeling the blood and tissue:air values in multiple linear regression analyses.
- Details on dosing and sampling:
- Liquid scintillation vials with a septum were used incubate the sample matrix with the test item. The test item was introduced into the vials as a vapour by injecting 1 mL of atmosphere from a gas sampling bag containing an air concentration of between 5000 and 10000 ppm. The concentration of the test item in the vials was 200 to 400 ppm. After heating (37°C) and vortexing at 200 to 300 rpm for 15 minutes, equilibrium in the test vial was reached. The headspace chemical concentrations were sampled then analysed by gas chromatography utilizing a flame ionization detector.
Results and discussion
Toxicokinetic / pharmacokinetic studies
Toxicokinetic parametersopen allclose all
- Toxicokinetic parameters:
- other: Human blood:air = 6.04±0.38
- Toxicokinetic parameters:
- other: Rat blood:air = 9.58±0.94
- Toxicokinetic parameters:
- other: Saline:air =1.41±0.04
- Toxicokinetic parameters:
- other: Olive oil:air = 178±6
- Toxicokinetic parameters:
- other: Rat fat:air = 148±11
- Toxicokinetic parameters:
- other: Rat liver:air = 8.96±0.61
- Toxicokinetic parameters:
- other: Rat muscle:air = 3.52±0.54
Metabolite characterisation studies
- Metabolites identified:
- not measured
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information): other: partition coefficients were determined for rat blood/tissues and human blood
This study and the conclusions which are drawn from it fulfil the quality criteria (validity, reliability, repeatability).
The techniques presented in this study offer a method for determining liquid & and tissue:air partition coefficients for volatile chemicals such as the test item. - Executive summary:
A technique was developed to determine partition coefficients for low-molecular-weight, volatile compounds such as the test substance. The liquid:air and tissue:air partitions were determined in 0.9% saline, in olive oil, and in blood, liver, muscle, and fat tissues from rats. Human blood:air values were also determined. The test substance, as a vapour, was mixed with the matrix then incubated for 15 minutes to allow for equilibration. Samples of the headspace were analysed by gas chromatography and the partition coefficient determined.
Values were:
Human blood:air = 6.04±0.38
Rat blood:air = 9.58±0.94
Saline:air =1.41±0.04
Olive oil:air = 178±6
Rat fat:air = 148±11
Rat liver:air = 8.96±0.61
Rat muscle:air = 3.52±0.54
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