Ricinoleyl monomaleate triglyceride

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

February 9 to March 4, 2000

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP study according to international guidelines. Analytical support was limited to measuring concentrations in the stock solutions.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2000

Report date:

2000

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

Test guideline 2

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

yes

Test material

Reference

Name:

Unnamed

Type:

Constituent

Details on test material:

Batch: T4649
Expiry date: 22 April 2001
Storage at 10 to 30 dC

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

The analytical method was suitable only for measurement of actual concentrations in samples taken from the stock solutions in acetone. Samples were also taken from the test media at the start and the end of the test. Analysis of the test concentration samples was attempted but resulted in most cases in non-dectable values. Therefore, final evaluation of the results was based on nominal concentrations.

Test solutions

Vehicle:

yes

Details on test solutions:

Identity and concentration of auxiliary solvent for dispersal: acetone 0.1 ml/l. Stock solutions in acetone contained 60 or 120 g/L. These were used to prepare the final test solutions.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

Less than 24 h old at the start of the tests.

Study design

Test type:

semi-static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Test conditions

Hardness:

213 - 237 mg CaCO3/L

Test temperature:

19.5 - 20.2

pH:

7.5 - 8.6

Dissolved oxygen:

70 - 93 %

Nominal and measured concentrations:

Nominal: 6 mg/L - Stock: 60 g/L - Actual: 57 - 60 g/L
Nominal: 12 mg/L - Stock: 120 g/L - Actual: 116 - 121 g/L

Reference substance (positive control):

not specified

Results and discussion

Effect concentrations

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

ca. 12 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: The concentration exceeded the solubility limit of the substance in water.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The test substance has proven to be non-toxic to invertebrates at levels at and above its solubility in water.