Registration Dossier
Registration Dossier
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EC number: 432-500-3 | CAS number: -
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 30 September to 9 December 1999
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP study according to international guidelines.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 000
- Report date:
- 2000
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.4-C (Determination of the "Ready" Biodegradability - Carbon Dioxide Evolution Test)
- GLP compliance:
- yes
Test material
Reference
- Name:
- Unnamed
- Type:
- Constituent
- Test material form:
- liquid: viscous
- Details on test material:
- Batch: T4649
Expiry date: 22 April 2001
Storage at 10 to 30 dC
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- Collected from one of the return lines at Burley Menston domestic sewage treatment works (Yorkshire water). On arrival in the lab, the sample was aerates and suspended solid concentration was determined by filtering with subsequent drying.
- Duration of test (contact time):
- 28 d
Initial test substance concentration
- Initial conc.:
- 15 mg/L
- Based on:
- other: Organic carbon
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- CO2 evolution
Reference substance
- Reference substance:
- benzoic acid, sodium salt
Results and discussion
% Degradationopen allclose all
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 68
- Sampling time:
- 28 d
- Remarks on result:
- other: Replicate 1
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 78
- Sampling time:
- 28 d
- Remarks on result:
- other: Replicate 2
- Details on results:
- Points of degradation plot (test substance): .5 % degradation after 2 d 3.5 % degradation after 8 d 8.5 % degradation after 15 d 26.5 % degradation after 20 d 44.5 % degradation after 23 d 59.5 % degradation after 26 d 71.5 % degradation after 28 d
BOD5 / COD results
- Results with reference substance:
- Points of degradation plot (reference substance):
40.5 % degradation after 2 d
63 % degradation after 8 d
70 % degradation after 15 d
73 % degradation after 20 d
75.5 % degradation after 23 d
78.5 % degradation after 26 d
83.5 % degradation after 28 d
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- readily biodegradable
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