Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 200-539-3 | CAS number: 62-53-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: oral
Administrative data
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Meets generally accepted scientific standards, well documented and acceptable for assessment
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 970
- Report date:
- 1970
Materials and methods
- Principles of method if other than guideline:
- The test substance is applied orally by gavage in graduated doses to seven groups of cats, one dose being used per group.
Subsequently, observations of deathss and clinical effects including the determination of methemoglobin level in the blood are made. - GLP compliance:
- no
- Test type:
- other: acute test
- Limit test:
- no
Test material
- Reference substance name:
- Aniline
- EC Number:
- 200-539-3
- EC Name:
- Aniline
- Cas Number:
- 62-53-3
- Molecular formula:
- C6H7N
- IUPAC Name:
- aniline
- Details on test material:
- - Name of test material (as cited in study report): aniline
- Analytical purity: p. a.
Constituent 1
Test animals
- Species:
- cat
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: BASF AG, Department of Toxicology
- Weight at study initiation: 2.4 kg (male), 1.9 kg (female)
- Diet (e.g. ad libitum): A soft feed made of cooked oat flakes, Latz-Purin and sodium chloride; sometimes the meat of whales; ad libitum
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- other: aqueous emulsion in traganth
- Details on oral exposure:
- VEHICLE
- Concentration in vehicle: 0.05 - 1% aqueous emulsion in traganth - Doses:
- 1.0, 2.6, 5.1, 10.2, 25.6, 51.1 and 102.2 mg/kg
- No. of animals per sex per dose:
- 2 animals per dose (two males, two females or one male and one female); in total 14 animals
- Control animals:
- no
- Details on study design:
- - Duration of observation period following administration: 3 days
- Frequency of observations and weighing: no data
- Necropsy of survivors performed: no
- Other examinations performed: clinical signs, methaemoglobin formation
Results and discussion
Effect levels
- Sex:
- male/female
- Dose descriptor:
- approximate LD50
- Effect level:
- >= 102 mg/kg bw
Any other information on results incl. tables
One out of two male cats died after administration of 102.2 mg/kg bw aniline. Light cyanosis was seen at day 1 after application of 2.6 mg/kg. Doses of 5.1, 10.2 and 25.6 mg/kg bw caused cyanosis in all animals. 51.1 mg/kg caused severe cyanosis with staggering gait, atony and vocalisation. In the high dose animals (102.2 mg/kg bw), severe cyanosis accompanied by accelerated breath and lateral position.
Animal |
Haemoglobin [%] Hours after administration |
||||||||
Dose [mg/kg] |
Pre-test |
2 |
4 |
6 |
24 |
30 |
48 |
72 |
|
102.2 |
1 |
0.5 |
74.1 |
71.2 |
79.9 |
28.2* |
- |
- |
- |
102.2 |
2 |
0.2 |
73.8 |
77.4 |
78.8 |
70.5 |
56.9 |
4.8 |
3.1 |
51.1 |
3 |
0 |
66.1 |
76.0 |
73.0 |
2.5 |
2.0 |
0 |
0 |
51.1 |
4 |
3.6 |
55.6 |
63.2 |
35.1 |
2.0 |
1.2 |
0.4 |
0.4 |
25.6 |
5 |
0 |
60.6 |
71.3 |
70.6 |
1.8 |
1.2 |
0.9 |
0 |
25.6 |
6 |
0 |
62.9 |
65.1 |
61.1 |
2.4 |
1.0 |
1.1 |
0 |
10.2 |
7 |
0 |
66.8 |
64.0 |
49.7 |
0.5 |
0.5 |
0.5 |
1.2 |
10.2 |
8 |
0 |
41.8 |
34.5 |
20.2 |
0.9 |
0.9 |
0.3 |
1.2 |
5.1 |
9 |
0 |
29.0 |
12.8 |
4.5 |
1.0 |
0.9 |
0 |
0.8 |
5.1 |
10 |
0 |
24.6 |
8.1 |
0 |
0.6 |
0 |
0 |
0 |
2.6 |
11 |
0 |
8.1 |
2.6 |
0.3 |
0 |
1.3 |
0.7 |
0.7 |
2.6 |
12 |
0.8 |
10.2 |
3.4 |
0 |
0.6 |
0 |
0 |
0 |
1.0 |
13 |
0.5 |
3.4 |
2.6 |
1.2 |
0.8 |
0 |
1.0 |
0 |
1.0 |
14 |
0.5 |
6.0 |
1.6 |
1.0 |
0.5 |
0 |
0 |
0 |
* Blood was taken after death.
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.