Glycerides, C8-18 and C18-unsatd.

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

migrated information: read-across based on grouping of substances (category approach)

Adequacy of study:

weight of evidence

Study period:

From Apr. 11, 1988 to Apr. 15, 1988

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: The study was conducted according to OECD Guideline 203. No analytical verification of test concentrations.

Justification for data waiving:

other:

Cross-reference

Cross-reference 1

Reason / purpose for cross-reference:

reference to same study

Cross-reference 2

Reason / purpose for cross-reference:

reference to other study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1988

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

not specified

Principles of method if other than guideline:

Not applicable

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Glycerides, C16 and C18-unsatd. and C18-unsatd. hydroxy

IUPAC Name:

Glycerides, C16 and C18-unsatd. and C18-unsatd. hydroxy

Details on test material:

- Name of test material (as cited in study report): Castor oil (CAS N° 8001-79-4, EC N° 232-293-8); under the SDA nomenclature, the name of this substance is ‘Glycerides, C16 and C18-unsatd. and C18-unsatd. hydroxy’

Specific details on test material used for the study:

Details on properties of test surrogate or analogue material (migrated information):
Not applicable

Sampling and analysis

Analytical monitoring:

no

Details on sampling:

No data

Test solutions

Vehicle:

no

Details on test solutions:

Stock solutions of the test material were prepared by mechanical dissolving (Ultra-Turrax) the appropriate amount of the test material in the required volume of deionised water. This stock solution was transferred into the test medium of one glass tank. Loading of the test medium with the test sample was done 2 h before the start of the test. Until the transfer of fish into the test solution, the contents of each glass tank were mixed thoroughly using a mechanical stirrer in order to facilitate the dispersing of the test sample.

Test organisms

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name: Zebra-fish
- Strain: Brachydanio rerio (Teleostei, Cyprinidae)
- Source: West Aquarium GmbH + Co. KG, D- 3422 Bad Lauterberg
- Age at study initiation (mean and range, SD): Two months (approx.)
- Length at study initiation (length definition, mean, range and SD): 2.0 ± 1.0 cm


ACCLIMATION
- Acclimation period: 13 d
- Acclimation conditions: The fish was transferred to a tank with a total water volume of about 200 L for adaptation to the environmental conditions during the test. During the adaptation period, the holding of the test fish was performed using a semi-static system in which the reconstituted water was recirculated and filtered continuously.
- Type and amount of food: Tetra Min Hauptfutter (Art.-No. 1004 DN, produced by Tetra-Werke, Dr. rer.nat. Ulrich Baensch GmbH, D- 4520 Melle 1)
- Feeding frequency: Twice daily
- Health during acclimation (any mortality observed): Good health, no mortality observed

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Post exposure observation period:

None

Test conditions

Hardness:

No data

Test temperature:

20.8-21.8

pH:

7.5-7.6

Dissolved oxygen:

>86% (at 96 h)

Salinity:

No data

Nominal and measured concentrations:

100 and 1000 mg/L (nominal)

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Material, size, headspace, fill volume: The glass tanks for the different test batches had the following dimensions: length 300 mm, width 200 mm and height 220 mm.
- Aeration: For the continuous aeration of the test medium in the different glass tanks special aquarium aerators, brand Optima (Art.-No. A 10007, produced by Weltweit GmbH & Co. KG, D- 2081 Holm near Hamburg) were used. Air was bubbled into the test medium through Pasteur pipettes directly connected with the aerator by flexible PVC tubes. The air-stream was regulated with tube-clamps providing for each tank a uniform air-stream of 1 to 3 bubbles per second.
- No. of organisms per vessel: 10


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of reconstituted water: It was prepared according to the Annex of OECD-Guideline 203 in the following way (referred to a total volume of 10.0 L):
117.6 g Calcium chloride dihydrate CaCl2.2 H2O (Art.No. 2382, produced by E. Merck AG, D- 6100 Darmstadt) were dissolved in deionised water; this solution was made up with deionised water to a total volume of 1.0 L.
49·3 g Magnesium sulfate heptahydrate MgSO 4.7 H2O (Art.-No. 5886 produced by E. Merck AG, D-6100 Darmstadt) were dissolved in deionised water; this solution was made up with deionisd water to a total volume of 1.0 L.
25.9 g Sodium hydrogencarbonate O 3 (Art.-No. 6329, produced by E. Merck AG, D-6100 Darmstadt) were dissolved in deionised water; this solution was made up with deionised water to a total volume of 1.0 L.
2.3 g Potassium chloride KCl (Art.-Noo 4936, produced by E. Merck AG, D-6100 Darmstadt) were dissolved in deionised water; this solution was made up with deionised water to a total volume of 1.0 L.
For the preparation of the reconstituted water, 25 mL each of the four afore-mentioned solutions were mixed; this mixture was then made up with deionised water to a total volume of 10.0 L Thus reconstituted water prepared in the described way, met the following requirements:
The sum of the calcium and magnesium ions was 2.5 mmol/L. The molar ratio of calcium to magnesium ions was 4:1. The molar ratio of sodium to potassium ions was 10:1. The total alkalinity or acid capacity was 0.8 mmol/L.
- Conductivity: < 0.1 µS/cm


OTHER TEST CONDITIONS
- Adjustment of pH: Yes, determination of pH was carried out with the measuring device pH 91/Set (Art.-No. 9.774091, produced by WTW, D-8120 Weilheim)


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Mortality rates, abnormal symptoms in the swimming behaviour, in visible physiological functions as well as in the outward appearance of the test fish were observed at 0, 24, 48, 72 and 96 h.

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

Charging the test medium with the test substance led to a flocky dispersion slowly settling up in the course of the test period.

Results with reference substance (positive control):

No data

Reported statistics and error estimates:

No data

Any other information on results incl. tables

Sublethal observations / clinical signs:

None

Applicant's summary and conclusion

Validity criteria fulfilled:

not specified

Conclusions:

Under the test conditions, the 96 h LC0 of castor oil for (Brachydanio rerio) was found to be 1,000 mg/L (based on nominal concentrations).

Executive summary:

A study was conducted to determine the lethal effects of castor oil to the Zebra-fish (Brachydanio rerio) using a static system according to the OECD Guideline 203.

Fish were exposed in separate test batches to different concentration (100 and 1000 mg/L) of the test material in the test medium over a test period of 96 h (each tank with 10 fish). Observations were made at 0, 24, 48, 72 and 96 h for the mortality rates, abnormal symptoms in the swimming behaviour, in visible physiological functions as well as in the outward appearance of the test fish. Fish were considered dead if touching of the caudal peduncle produced no reaction and if no breathing movements could be observed.

Under the test conditions, the 96 h LC0 for (Brachydanio rerio) was found to be 1,000 mg/L (based on nominal concentrations).

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