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Diss Factsheets
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EC number: 484-470-6 | CAS number: 623-40-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material (migrated information):
As noted in the hydrolysis section, oximesilanes quicky hydrolyze to the corresponding oxime and reactive silanols which spontaneously cross link to form high molecular weight silicone polymers. The relevant hydrolysis product is MPKO, the substance that was tested.
Data on selected oximes and oxime silanes are summarized in the attached Table. Although data for each endpoint is not available for all products, the available data show similarities between oxime silanes and their hydrolysis products (oxime). These materials show low toxicity to fish, and daphnia. For algal studies, the toxicity shows a structure activity relationship. The smaller oximes are more toxic to alga, the larger oximes and associated oxime silanes are less toxic. The similarities in response between the oximes and oxime silanes provide additional support of the silicone containing moieties do not contribute to the aquatic toxicity of these materials. Due to the rapid hydrolysis, it is appropriate to use the oxime as surrogate data for the oxime silane. - Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: 0, 5.6, 10, 18, 32, 56, and 100 mg/l at 0 and 72 h.
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The nominal 100 mg l-1 test concentration of methyl propyl ketoxime was prepared in 2000 ml of culture medium. This stock solution was stirred for 30 minutes using a magnetic follower, after which the stock solution was observed to be clear and colourless. - Test organisms (species):
- Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- TEST ORGANISM
- Strain: ATCC 22662
- Source (laboratory, culture collection): laboratory cultures maintained under axenic conditions
- Age of inoculum (at test initiation): 4 days during exponential growth phase
- Culturing media and conditions: same as test
- Any deformed or abnormal cells observed: non observed - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
- Hardness:
- not reported
- Test temperature:
- 18-22 °C
- pH:
- 7.6 - 7.8
- Dissolved oxygen:
- not reported
- Nominal and measured concentrations:
- Nominal test substance concentrations: 5.6, 10, 18, 32, 56 and 100 mg/L
With the exception of the 5.6 mg/l samples, the measured concentrations with 91 - 100% of nomimal - Details on test conditions:
- TEST SYSTEM
- Test vessel: 250 ml glass conical flasks closed with foam bungs filled with 100 ml of test solution and 0.75 ml of inoculum culture
- Initial cells density: 0.5 x 10e4 cells/ml
- Control end cells density: at 72 hours, 81.8 x 10e4 cells/ml
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 6
GROWTH MEDIUM
- Standard medium used: yes
OTHER TEST CONDITIONS
- Sterile test conditions: no
- Adjustment of pH: no
- Photoperiod: continuous
- Light intensity and quality: "cool-white" illumination of 6708 lux (mean)
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: electronic particle counter
- Reference substance (positive control):
- no
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- ca. 54 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 32 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 88 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 32 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Details on results:
- - Exponential growth in the control (for algal test): yes
- Observation of abnormalities (for algal test): At the 32, 56 and 100 mg l-1 test concentrations the cells were observed to be smaller than those in the control test concentration.
- Any stimulation of growth found in any treatment: none observed
- Effect concentrations exceeding solubility of substance in test medium: none observed - Reported statistics and error estimates:
- one way analysis of variance and Duunnett's procedure to identify significant changes from control. Weibull method used to estimate the meane effective concentrations.
- Validity criteria fulfilled:
- yes
- Remarks:
- Please refer to Table 1 at "Any other information on results incl. tables"
- Conclusions:
- The 72 hr ECy50 is 54 mg/l, and the ECr50 is 88 mg/l. The 72 hour no-observed effect concentration was 32 mg/l based on yield and growth rate.
Reference
Table 1: Validity criteria for OECD 201.
Criterion from the guideline |
Outcome |
Validity criterion fulfilled |
The biomass in the control cultures should have increased exponentially by a factor of at least 16 within the 72-hour test period. |
169 |
yes |
The mean coefficient of variation for section-by-section specific growth rates (days 0-1, 1-2 and 2-3, for 72-hour tests) in the control cultures must not exceed 35% |
7.9 % |
yes4 |
The coefficient of variation of average specific growth rates during the whole test period in replicate control cultures must not exceed 7% in tests with Pseudokirchneriella subcapitata and Desmodesmus subspicatus. For other less frequently tested species, the value should not exceed 10%. |
4.0 % |
yes |
Description of key information
ErC50 (72 h): 88 mg/L (nominal, OECD 201)
NOErC (72 h): 32 mg/L (nominal, OECD 201)
Key value for chemical safety assessment
Additional information
The toxicity to algae was tested in a GLP-study following OECD guideline 201. Green algae (Pseudokirchneriella subcapitata) were exposed to nominal test substance concentrations of 5.6, 10, 18, 32, 56 and 100 mg/L. The analytical dose verification at 0 and 72 h was conducted using a HPLC method. With the exception of the 5.6 mg/l samples, the mean measured concentrations in the range of 91 - 100% of nominal were determined. The mean growth rate at the substance concentrations 56 mg/L and 100 mg/L was significantly reduced compared to the control growth rate. Thus, the determined ErC50 (72 h) was 88 mg/L (nominal) and the NOErC (72 h) was 32 mg/L.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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