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Diss Factsheets
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EC number: 484-470-6 | CAS number: 623-40-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Details on sampling:
- The concentrations of methyl propyl ketoxime in the test solution were measured at 0 and 96 hours using a high performance liquid chromatography method.
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
Test material weighed, added to water in beaker, stirred vigourously for 10 minutes. (concentration 100 mg/l)
Final solutions were clear and colorless. - Test organisms (species):
- Oncorhynchus mykiss (previous name: Salmo gairdneri)
- Details on test organisms:
- TEST ORGANISM
- Common name:rainbow trout
- Strain: O. mykiss
- Source: Houghton Springs Fish Farm, Winterbourne Houghton, Blandford Forum, Dorset DT11, 0PD
- Length at study initiation : 44 - 52 mm, mean 49 mm
- Weight at study initiation : range 1.10 – 1.96 g, mean 1.55 g
- Feeding during test: none
ACCLIMATION
- Acclimation period: at least 7 days
- Acclimation conditions: same as test
- Type and amount of food: proprietary brand of high protein pelleted fish food
- Feeding frequency:daily
- Health during acclimation : no mortality observed - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 96 h
- Post exposure observation period:
- na
- Hardness:
- 46.3 mg/l CaCO3
- Test temperature:
- 14.5 - 15.2 °C
- pH:
- 7.2 - 7.57
- Dissolved oxygen:
- 73 - 92%
- Salinity:
- NA
- Nominal and measured concentrations:
- nominal 100 mg/l, measured 102 mg/l at 0 hour and 103 mg/l at 96 hours
- Details on test conditions:
- TEST SYSTEM
- Test vessel: glass tanks (external dimensions L×W×H;610×305×310)
- Material, size, headspace, fill volume: working volume of 30 litres
- Aeration: gentle aeration
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water:tap water coarsely filtered to remove particulate material, passed through activated carbon and dechlorinated using sodium thiosulphate. Salts ifrequired to maintain minimum hardness levels. Treated water passed through ultraviolet steriliser and filtered to 10 µm
- Total organic carbon: 2.35 mg/l dissolved organic carbon
- Particulate matter: < 3 mg/l as suspended solids
- Metals: not reported
- Pesticides: < 0.01 ug/l
- Chlorine: < 2 u/l as Cl2 (free and total)
- Alkalinity:26.4 m/l CaCO3
- Ca/mg ratio: not reported
- Conductivity: 213 us/cm
- Culture medium different from test medium:
- Intervals of water quality measurement: daily to quarterly depending on parameter
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Mortality and symptoms of toxcitiy after 3, 24, 48, 72 and 96 hours
OTHER TEST CONDITIONS
- Adjustment of pH:Not needed
- Photoperiod: 16 h light, 8 h darkness
TEST CONCENTRATIONS
- Spacing factor for test concentrations: NA limit test
- Range finding study: yes
- Test concentrations: 10 and 100 mg/l
- Results used to determine the conditions for the definitive study: 1 dead at 10 mg/l, none at 100 mg/l. - Reference substance (positive control):
- not required
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- ca. 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: mortality and signs of toxicity
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Details on results:
- - Behavioural abnormalities: none observed
- Observations on body length and weight: none observed
- Other biological observations: none observed
- Mortality of control: none observed
- Other adverse effects control: none observed
- Abnormal responses: none observed
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: none observed
- Effect concentrations exceeding solubility of substance in test medium: none observed - Results with reference substance (positive control):
- NA
- Reported statistics and error estimates:
- NA
- Sublethal observations / clinical signs:
Table 1: Validity criteria for OECD 203.
Criterion from the guideline
Outcome
Validity criterion fulfilled
The mortality in the control(s) should not exceed 10% (or one fish if less than ten are used) at the end of the test
0%
yes
The dissolved oxygen concentration must have been at least 60 per cent of the air
saturation value throughout the test
68 to 92%
yes
There must be evidence that the concentration of the substance being tested has been satisfactorily maintained, and preferably it should be at least 80% of the
nominal concentration throughout the test. If the deviation from the nominal
concentration is greater than 20%, results should be based on the measured
concentration.
102-103% of nominal
yes
- Validity criteria fulfilled:
- yes
- Remarks:
- Please refer to Table 1 at "Any other information on results incl. tables"
- Conclusions:
- No mortality or signs of toxicity were observed in raindbow trout exposed to 100 mg/l (nominal; 102 mg/l measured) test substance for 96 hours.
Reference
Description of key information
LC50 (96 h) > 100 mg/L (nominal, OECD 203).
Key value for chemical safety assessment
Additional information
The acute toxicity of 2-Pentanone oxime to fish was tested in a GLP guideline study following OECD 203. The study was conducted as a static limit test at a nominal test substance concentration of 100 mg/L. The substance concentration was analytically verified by HPLC. The measured test substance concentrations were 102 mg/L at test start and 103 mg/L at test end after 96 hours. Ten fish of the species Oncorhynchus mykiss were used as test organisms. Mortality or symptoms of toxicity were not observed. The reported LC50 (96 h) is > 100 mg/L (nominal).
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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