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EC number: 217-168-8 | CAS number: 1761-71-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Justification for type of information:
- GLP guideline study (ISO 8192) of the structural analogue DM-PACM (CAS 6864-37-5). PACM and DM-PACM are considered read-across analogues based on structural similarity and similar physico-chemical and toxicological properties. The common structural features of the two substances are: a common functional primary amine group; the amines are bound to a cyclic aliphatic organic substituent; there are no elements other than carbon, hydrogen and nitrogen; identical structures except for a methyl group on each cyclohexane, ortho to the amine on DM-PACM similar molecular weights, both under 500 daltons, qualifiyng as “low molecular weight” compounds. For a detailed read across justification see also the attached justification document.
- Reason / purpose for cross-reference:
- read-across source
- Duration:
- 30 min
- Dose descriptor:
- other: EC20
- Effect conc.:
- 160 mg/L
- Nominal / measured:
- nominal
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Duration:
- 30 min
- Dose descriptor:
- other: Highest test concentration below a respiration inhibition of 20%
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Validity criteria fulfilled:
- yes
- Conclusions:
- Read across to target substance DMDC: EC20 of 160 mg/l was determined
- Executive summary:
The analogue chemical DMDC shows comparable physico-chemical behavior, ecotoxicity and toxicity to that of the target chemical PACM. Available data on aquatic toxicity indicate that toxicity is generally slightly lower for PACM than for its analogue DM-PACM. Therefore, read across to ecotoxicity data from DMDC is justified(for detailled justification see field "justification for type of information")and can be used in a worst-case approach.
A short-term respiration inhibition study according to ISO 8192 with domestic activated sludge was conducted. After 30 minutes an EC20 of 160 mg/l was determined.
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Study period:
- 1987-08-20
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study without detailed documentation
- Qualifier:
- according to guideline
- Guideline:
- other: German Industrial Standard DIN 38412, Part 27 (draft): ROBRA- Test; Test on inhibition of oxygen consumption
- GLP compliance:
- no
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Stock solution (10000 mg/L, pH 11.8): stirred for 20 h.
- Evidence of undissolved material (e.g. precipitate, surface film, etc): no - Test organisms (species):
- Pseudomonas putida
- Details on inoculum:
- - Laboratory culture: yes
- Initial biomass concentration: extinction (E: 436 nm/1 cm; dilution of 1:100) = 0.320
- Age of bacterial suspension: 22 h - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 30 min
- Test temperature:
- 25 °C
- pH:
- 7.2 (lowest test conc.) to 11.8 (highest test conc. and stock solution)
- Nominal and measured concentrations:
- nominal: 0 (control), 19.5, 39, 78, 156, 312, 625, 1250, 2500, 5000, and 10000 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel:
- Material, size, headspace, fill volume: glass vessels with flattened bottom and silicon-foam caps, 10 mL fill volume
- No. of organisms per vessel: 1 mL of bacterial pre-culture (10 TE/F)
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 2
- TEST MEDIUM: AK-Medium according to DIN 38412, Part 8 (draft)
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Fluorometric determination of growth as extinction at 436 nm after 17 h
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2 - Duration:
- 30 min
- Dose descriptor:
- EC10
- Effect conc.:
- 80 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Remarks on result:
- other: based on not pH-adjusted test solutions
- Duration:
- 30 min
- Dose descriptor:
- EC50
- Effect conc.:
- ca. 156 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Remarks on result:
- other: not reported, but taken from presented data.
- Conclusions:
- Based on the nominal concentrations of the test item, EC10 in the Pseudomonas cell multiplication inhibition test (ROBRA) was 80 mg/L and the EC50 was 156 mg/L.
- Executive summary:
The toxicity of the test item was examined according to German Industrial Standard DIN 38412, Part 27 (draft): ROBRA- Test; Test on inhibition of oxygen consumption
Psaudomonas putida was exposed with ten nominal test concentrations (Blank, 19.5, 39, 78, 156, 312, 625, 1250, 2500, 5000, 10000 mg/L) of the test item and the reduction of oxygen consumption was determined.
Based on the nominal concentrations of the test item, EC10 in the Pseudomonas cell multiplication inhibition test (ROBRA) was 80 mg/L and the EC50 was 156 mg/L.
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study with acceptable restrictions
- Remarks:
- e.g. activated sludge from industrial source used instead of municipal wwtp
- Qualifier:
- according to guideline
- Guideline:
- ISO 8192 (Water quality - Test for inhibition of oxygen consumption by activated sludge for carbonaceous and ammonium oxidation)
- Version / remarks:
- part determination of respiration inhibition of activated sludge
- Deviations:
- not specified
- GLP compliance:
- no
- Analytical monitoring:
- no
- Vehicle:
- no
- Test organisms (species):
- activated sludge, industrial
- Details on inoculum:
- - Laboratory culture: Activated sludge from the industrial wastewater treatment plant of BASF, production site Ludwigshafen, Germany
- Test type:
- static
- Water media type:
- freshwater
- Remarks:
- inocolum: activated sludge from industrial plant
- Limit test:
- no
- Total exposure duration:
- 180 min
- Test temperature:
- ambient
- Nominal and measured concentrations:
- nominal: 0 (control), 100, 500, 1000 mg/L
Stock solution 3000 mg/L - Details on test conditions:
- syntheticsewage consisting of peptone, meat extract,urea, sodium chloride, 7 mg; calcium chloride, magnesium sulphate deionised water
- Reference substance (positive control):
- no
- Duration:
- 30 min
- Dose descriptor:
- other: EC20
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Duration:
- 180 min
- Dose descriptor:
- EC50
- Effect conc.:
- ca. 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Details on results:
- No inhibition after 30 min-exposure even at the highest concentration (1000 mg/L)
- Conclusions:
- Based on the nominal concentrations of the test item, 30 min EC20 in the Test for inhibition of oxygen consumption by activated sludge according to ISO 8192 was >1000 mg/L and the 180 min EC50 was approximately 100 mg/L.
- Executive summary:
The toxicity of the test item was examined by determination of respiration inhibition of activated sludge according to ISO 8192.
Activated sludge was exposed with 3 nominal test concentrations (Blank, 100, 500, 1000 mg/L) of the test item and the reduction of oxygen consumption was determined.
Based on the nominal concentrations of the test item, EC20 was 80 mg/L and the EC50 was >1000 mg/L and the 180 min EC50 was approximately 100 mg/L.
Referenceopen allclose all
Results on activated sludge respiration over 30 min
Parameters |
Nominal concentration [mg/L] |
||||
10 |
50 |
100 |
500 |
1000 |
|
DOC concentration of the test item [mg/L] |
7 |
37 |
74 |
570 |
740 |
Respiration speed [mg/L*h] |
25 |
23 |
21 |
18 |
18 |
Respiration inhibition [%] compared to blank value* |
0 |
8 |
16 |
28 |
28 |
*significant effect > 20% inhibition compared to blank value
Respiration
Test substance conc. (mg/L) |
respiration rate: replicate 1 (mg/mL*min) |
respiration rate: replicate 2 (mg/mL*min) |
respiration rate: mean value (mg/mL*min) |
% of Control |
pH |
C |
2.3 |
2.10 |
2.200 |
100 |
|
19.5 |
>C |
>C |
>100 |
>100 |
7.2 |
39 |
>C |
>C |
>100 |
>100 |
7.2 |
78 |
2.15 |
2.25 |
2.200 |
100 |
7.2 |
156 |
1.15 |
1.15 |
1.150 |
52.3 |
7.9 |
312 |
0 |
0 |
0 |
0 |
10.1 |
625 |
0 |
0 |
0 |
0 |
10.5 |
1250 |
0 |
0 |
0 |
0 |
10.7 |
2500 |
0 |
0 |
0 |
0 |
11.1 |
5000 |
0 |
0 |
0 |
0 |
11.3 |
10000 |
0 |
0 |
0 |
0 |
11.8 |
C = Blank
Duration of incubation: 30 min
Test concentration |
Oxygen concentration (mg/L) |
Respiration rate |
Respiration change |
||
(mg/L; nominal) |
start |
end |
difference |
[mg O2/L/h] |
[%] |
0 (blank) |
4.61 |
2.65 |
1.96 |
29.4 |
0.130 |
100 |
4.95 |
3.32 |
1.64 |
24.5 |
-16.5 |
500 |
5.33 |
3.60 |
1.73 |
26.0 |
-11.5 |
1000 |
4.53 |
2.82 |
1.71 |
25.6 |
-12.8 |
Duration of incubation: 180 min
Test concentration |
Oxygen concentration (mg/L) |
Respiration rate |
Respiration change |
||
(mg/L; nominal) |
start |
end |
difference |
[mg O2/L/h] |
[%] |
0 (blank) |
5.39 |
3.80 |
1.59 |
23.8 |
0.040 |
100 |
6.40 |
5.46 |
0.943 |
14.1 |
-40.6 |
500 |
6.75 |
6.30 |
0.454 |
6.81 |
-71.4 |
1000 |
6.76 |
5.76 |
1.00 |
15.0 |
-36.9 |
Description of key information
- Read across from a short-term respiration inhibition study according to ISO 8192 with the structural analogue DMDC and domestic activated sludge was used as key study. After 30 minutes an EC20 of 160 mg/l was determined.
The read across result is supported by two experimental studies with PACM:
- Pseudomonas putida 30 -min respiration rate EC10 was 80 mg/L.
- Respiration inhibition study with activated sludge from industrial WWTP, the 30 -min EC20 was > 1000 mg/L.
Key value for chemical safety assessment
Additional information
Read across to structural analogue substance:
The analogue chemical DMDC shows comparable physico-chemical behaviour, ecotoxicity and toxicity to that of the target chemical PACM. Available data on aquatic toxicity indicate that toxicity is generally slightly lower for PACM than for its analogue DMDC. Therefore, read across to ecotoxicity data from DMDC is justified (for detailed justification see field "justification for type of information" in the study endpoint record for the target chemical PACM) and can be used in a worst-case approach.
A short-term respiration inhibition study according to ISO 8192 with DMDC and domestic activated sludge was conducted. After 30 minutes an EC20 of 160 mg/l was determined.
The read across result from DMDC revealing low toxicity towards microorganisms is supported by two experimental studies with PACM:
In a study with Pseudomonas putida a 30 -min EC10 was determined to be 80 mg/L based on the respiration rate (BASF AG, 1987; report no. 01/87/0792).
In a short-term respiration inhibition study with activated sludge from a WWTP treating industrial wastewater, the 30 -min EC20 was determined to be > 1000 mg/L (BASF AG, 1986; report no. 01.114/86).
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