Tripropylamine

Acute Toxicity: inhalation

Administrative data

Endpoint:

acute toxicity: inhalation

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1975-06-18 to 1975-08-21

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: no GLP, brief, little information on test method (e.g. no information on acclimatisation, weight of animals, lighting)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1975

Report date:

1975

Materials and methods

Test guideline

Qualifier:

no guideline followed

Principles of method if other than guideline:

Ten male, albino rats were placed in a 70- litre, all glass exposure chamber and exposed to a saturated atmosphere of the test material in air for one hour. The material was administered as an aerosol with particles 3-5 microns in diameter. The rats were subjected to average concentrations of 4.5 mg/L, 7.4 mg/L or 12.8mg/L during the exposure period.

GLP compliance:

not specified

Test type:

standard acute method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

Tripropylamine

EC Number:

203-047-7

EC Name:

Tripropylamine

Cas Number:

102-69-2

Molecular formula:

C9H21N

IUPAC Name:

tripropylamine

Test material form:

other: liquid

Details on test material:

- Name of test material (as cited in study report): Tri-n-propylamine
- Substance type: tertiary amine / aliphatic amine

Test animals

Species:

rat

Strain:

other: albino

Sex:

male

Details on test animals or test system and environmental conditions:

no data available

Administration / exposure

Route of administration:

inhalation: aerosol

Type of inhalation exposure:

whole body

Vehicle:

other: desiccated air

Details on inhalation exposure:

GENERATION OF TEST ATMOSPHERE / CHAMBER DESCRIPTION
- Exposure apparatus: all glass exposure chamber
- Exposure chamber volume: 70 litres
- Method of holding animals in test chamber: free
- Source and rate of air: air was passed through a desicant prior to being passed through the test material, the rate of flow was 36 litres per minute (4.5 mg/L) or 43 litres per minute (7.4mg/L) and 25.9 litres per minute (12.8 mg/L)
- Method of conditioning air: air was passed through a desicant prior to being passed through the test material.
- System of generating particulates/aerosols: the material was administered as an aerosol with particles 3~5 microns in diameter.
- Temperature, humidity, pressure in air chamber: 72°F

TEST ATMOSPHERE
- Brief description of analytical method used: The air was passed through a deslcant prior to being passed through the test material. By differential weighing it was calculated that the rats were subjected to a concentration of 4.5 mg/L, 7.4 mg/L or 12.8 mg/L during the exposure period. These are averages values over the one hour period.
- Samples taken from breathing zone: no data

TEST ATMOSPHERE (if not tabulated)
- MMAD (Mass median aerodynamic diameter) / GSD (Geometric st. dev.): particles 3-5 microns in diameter

Analytical verification of test atmosphere concentrations:

not specified

Duration of exposure:

1 h

Concentrations:

4.5 mg/L, 7.4 mg/L, 12.8 mg/L

No. of animals per sex per dose:

10 male

Control animals:

not specified

Details on study design:

- Duration of observation period following administration: 14 days
- Frequency of observations and weighing: not given
- Necropsy of survivors performed: no data
- Other examinations performed: clinical signs

Results and discussion

Effect levels

Sex:

male

Dose descriptor:

LC50

Effect level:

ca. 4.5 other: mg/L

Exp. duration:

1 h

Mortality:

4.5 mg/L: 6 rats died
7.4 mg/L: all rats died within 40 min
12.8 mg/L: all rats died within 35 min

Clinical signs:

other: 4.5 mg/L: within 30 min all rats exhibited body tremors, by 40 min the rats were inactive; the tremors ceased within 24 hours 7.4 mg/L: within 20 min all the rats exhibited vigorous pawing and then tremors, by 30 min all rats exhibited tremors 12.8 mg/L:

Body weight:

no data

Gross pathology:

no data

Other findings:

no other findings reported

Any other information on results incl. tables

Dose of 4.5 mg/L:

Within 30 minutes all rats exhibited body tremors. By 40 minutes the rats were inactive. Within 60 minutes 2 rats were dead and the remaining rats were still exhibited tremors. Within 15 minutes after exposure 4 additional rats died. Tremors ceased within 24 hours and there were no additional deaths during the 14 day observation period. A total of 6 rats died during the 14 day observation period.

Dose of 7.4 mg/L:

Within 20 minutes the rats exhibited vigorous pawing and then tremors. By 30 minutes all rats exhibited tremors. By 40 minutes all 10 rats were dead.

Dose of 12.8 mg/L:

Initially the rats were more active than normal but soon they exhibited decreased activity. Within 10 minutes they exhibited whole body tremors and after 20 minutes the rats were still twitching. By 35 minutes all 10 rats were dead.

Applicant's summary and conclusion

Interpretation of results:

Toxicity Category III

Remarks:

Migrated information Criteria used for interpretation of results: EU

Conclusions:

The study was performed without following a specific Guideline and still considered to be of the high quality (reliability Klimisch 2). The test material did induce signs of acute inhalation toxicity in rats under the conditions of the test. LC50 of 4.5 mg/L generated using 1 hour exposure corresponds to a 4-hour LC50 of 2.25 mg/L (obtained by dividing by a factor of 2). LC50 of 2.25 mg/L points to Cat. 3 (H331 Toxic if inhaled).

Executive summary:

A study on the acute inhalation toxicity of tri-n-propylamine (aerosol) for rats was conducted. The exposure was conducted via whole-body exposure of groups of 10 male albino rats to an aerosol (doses: 4.5 mg/L, 7.4. mg/L and 12.8 mg/L) for a single 1-hour period. The particles were 3-5 microns in diameter. After exposure the animals were observed for 14 days. At 12.8 mg/L the rats exhibited initially an increased activity than normal but soon they exhibited decreased activity. Within 10 minutes they exhibited whole body tremors and after 20 minutes the rats were still twitching. By 35 minutes all 10 rats were dead. At 7.4 mg/L the rats exhibited within 20 minutes vigorous pawing and then tremors. By 30 minutes all rats exhibited tremors. By 40 minutes all 10 rats were dead. At 4.5 mg/L all rats exhibited body tremors within 30 minutes. By 40 minutes the rats were inactive. Within 60 minutes 2 rats were dead and the remaining rats were still exhibited tremors. Within 15 minutes after exposure 4 additional rats died. Tremors ceased within 24 hours and there were no additional deaths during the 14 day observation period. A total of 6 rats died during the 14 day observation period. No information is given on body weight changes or pathological examinations.The study thus shows that the test substance has a high acute inhalation toxicity. In conclusion, tri-n-propylamine was found to have a LC50 (median lethal dose) of approximately 4.5 mg./L for albino rats and thus would be considered toxic (acute tox. cat. 3).