2-methylbut-3-yn-2-ol

Skin irritation / corrosion

Administrative data

Endpoint:

skin irritation: in vivo

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Comparable to guideline study with acceptable restrictions

Cross-reference

Reason / purpose for cross-reference:

reference to same study

Data source

Reference

Reference 1

Reference Type:

other company data

Title:

Unnamed

Year:

1966

Report date:

1966

Reference 2

Reference Type:

secondary source

Title:

Unnamed

Year:

2000

Reference 3

Reference Type:

secondary source

Title:

SIDS Initial Assessment Report - 2-Methylbut-3-yn-ol

Author:

BASF AG

Year:

2002

Bibliographic source:

OECD SIDS

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 404 (Acute Dermal Irritation / Corrosion)

Principles of method if other than guideline:

Internal BASF method was used, which was in large part equivalent to methods described in OECD Guideline 404

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

2-methylbut-3-yn-2-ol

EC Number:

204-070-5

EC Name:

2-methylbut-3-yn-2-ol

Cas Number:

115-19-5

Molecular formula:

C5H8O

IUPAC Name:

2-methylbut-3-yn-2-ol

Details on test material:

- Analytical purity: 98.5%

Test animals

Species:

rabbit

Strain:

Vienna White

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Weight at study initiation: 2.9, 2.78 kg

Test system

Type of coverage:

occlusive

Preparation of test site:

other: clipped

Vehicle:

unchanged (no vehicle)

Controls:

other: other side of same animal, treated with saline

Amount / concentration applied:

TEST MATERIAL
- Amount(s) applied (volume or weight with unit): 0.2 ml
- Concentration (if solution): 100%

Duration of treatment / exposure:

1 min, 5 min, 15 min, 20 h

Observation period:

5 days

Number of animals:

2

Details on study design:

TEST SITE
- Area of exposure: 2.5 cm x 2.5 cm of back skin
- Type of wrap if used: cotton pad (seize: 2.5 cm x 2.5 cm) saturated with the undiluted test substance (ca. 0.5 ml)


REMOVAL OF TEST SUBSTANCE
- Washing (if done): 50% Lutrol (polyethylenglycol) dilution in water
- Time after start of exposure: 1, 5, 15 min and 20 h


SCORING SYSTEM:
Erythema and edema were scored according the below described system:
Although the results in the report were originally not given as Draize scores the data can be transferred into the Draize scoring system (in brackets). (+) = none - negligible effect (0)
+ = slight effect (1)
++ = moderate effect (2)
+++ = severe effect (>= 3)
N = necrosis

Results and discussion

In vivo

Results

Results 1

Irritation parameter:

erythema score

Basis:

mean

Time point:

other: 24 - 72 h

Score:

0.3

Max. score:

4

Reversibility:

fully reversible

Remarks on result:

other: after 20 h application

Results 2

Irritation parameter:

edema score

Basis:

mean

Time point:

other: 24 - 72 h

Score:

0

Max. score:

4

Reversibility:

fully reversible

Remarks on result:

other: after 20 h application

Any other information on results incl. tables

Irritation scores acc. Draize::

Readings	Animal	Exposure period: 1 min			Exposure period: 5 min			Exposure period: 15 min			Exposure period: 20 h
		Erythema	Edema	Additional findings	Erythema	Edema	Additional findings	Erythema	Edema	Additional findings	Erythema	Edema	Additional findings
1h	1	0	0		0	0		0	0		0	0
	2	0	0		0	0		0	0		0	0
24 h	1	0	0		0	0		0	0		2	0	overlapping  test site
	2	0	0		0	0		0	0		0	0
48 h	1	0	0		0	0		0	0		0	0
	2	0	0		0	0		0	0		0	0
72 h	1	0	0		0	0		0	0		0	0
	2	0	0		0	0		0	0		0	0
96 h	1	0	0		0	0		0	0		0	0
	2	0	0		0	0		0	0		0	0
Mean 24 - 72 h	1	0.0	0		0.0	0		0.0	0		0.7	0
	2	0.0	0		0.0	0		0.0	0		0.0	0
Mean		0	0		0	0		0	0		0.3	0

Applicant's summary and conclusion

Interpretation of results:

not irritating

Remarks:

Migrated information Criteria used for interpretation of results: EU